Multimodal Freight Transport (MFT) has been introduced as a solution for reducing the external costs of freight transport while achieving cost improvements. Despite the MFT benefits, its share has been low in practice, and transport by trucks remains the most preferred transport mode. A few works have recently investigated this issue by discussing various barriers to MFT. However, little conceptual work comprehensively examines the barriers that organizations may face during MFT applications. To address this gap, this paper has reviewed 104 studies and identified 31 barriers and possible strategies for overcoming them. To clarify the nature of these barriers, we developed a conceptual barrier framework that positions the identified barriers within the overall MFT chain. This framework categorizes the barriers into six categories: MFT terminal, MFT network, management, regulations and subsidies, delivery characteristics, and interoperability. The findings provide decision-makers and practitioners with theoretical and practical insights into the barriers to transition toward MFT and will assist them in implementing MFT successfully.