Is  Tuber melanosporum  colonizing the roots of herbaceous, non-ectomycorrhizal plants?

Schneider‐Maunoury, Laure; Leclercq, Sébastien; Clément, Cannelle; Covès, Hervé; Lambourdière, Josie; Sauve, Mathieu; Richard, Franck; Selosse, Marc‐André; Taschen, Elisa

doi:10.1016/j.funeco.2017.10.004

Cited by 42 publications

(58 citation statements)

References 62 publications

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“…An increasing number of molecular studies on root‐associated fungal communities are indicating that the taxonomic and functional diversity of these communities are extremely varied (Menkis et al , 2005; Vasiliauskas et al , 2007; Toju et al , 2013; Kohout et al , 2018; Schneider‐Maunoury et al , 2018). Surprisingly, species of Mycena , a genus known to be important in C and N cycling from plant litter and wood (Boberg et al , 2008; Osono, 2020), are often amongst the most abundant taxa recovered from the roots of a range of plant species (Bougoure et al , 2007; Blaalid et al , 2012; Botnen et al , 2014; Lorberau et al , 2017), and several studies report orchid mycorrhizal associations by Mycena species (Martos et al , 2009; Ogura‐Tsujita et al , 2009; Zhang et al , 2012).…”

Section: Discussionmentioning

confidence: 99%

In vitro evidence of root colonization suggests ecological versatility in the genus Mycena

et al. 2020

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Summary The root‐associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root‐associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. In all, 17 Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were investigated using 14C and 32P to show potential transfer between symbionts. All Mycena species associated closely with fine roots, showing hyphal penetration into the roots, which in some cases were intracellular. Seven species formed mantle‐like structures around root tips, but none formed a Hartig net. Mycena pura and Mycena galopus both enhanced seedling growth, with M. pura showing significant transfer of 32P to the seedlings. Our results support the view that several Mycena species can associate closely with plant roots and some may potentially occupy a transitional state between saprotrophy and biotrophy.

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Section: Discussionmentioning

confidence: 99%

In vitro evidence of root colonization suggests ecological versatility in the genus Mycena

et al. 2020

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“…melanosporum ) but never on T . borchii (Rubini et al ., ; Riccioni et al ., ; Murat et al ., ; Molinier et al ., , ; Taschen, et al ., ; De la Varga et al ., ; Schneider‐Maunory et al ., ). In this study, T .…”

Section: Discussionunclassified

Ascoma genotyping and mating type analyses of mycorrhizas and soil mycelia of Tuber borchii in a truffle orchard established by mycelial inoculated plants

Leonardi

Murat

Puliga

et al. 2019

Environmental Microbiology

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Summary Tuber borchii (the Bianchetto truffle) is a heterothallic Ascomycete living in symbiotic association with trees and shrubs. Maternal and paternal genotype dynamics have already been studied for the black truffles Tuber melanosporum and Tuber aestivum but not yet for T. borchii. In this study, we analysed maternal and paternal genotypes in the first truffle orchard realized with plants inoculated with five different T. borchii mycelia. Our aims were to test the persistence of the inoculated mycelia, if maternal and/or paternal genotypes correspond to inoculated mycelia and to assess the hermaphroditism of T. borchii. The mating type of each isolate as well as those of mycorrhizas, ascomata and extraradical soil mycelia was determined. Moreover, simple sequence repeat (SSR) profiles of maternal and paternal genotypes were assessed in 18 fruiting bodies to investigate the sexual behaviour of this truffle. The maternal genotypes of the fruiting bodies corresponded to those of the inoculated mycelia with only two exceptions. This confirmed that the inoculated mycelia persisted 9 years after plantation. As regards paternal partner, only two had the same genotype as those of the inoculated mycelia, suggesting hermaphroditism. Most of the new paternal genotypes originated from a recombination of those of inoculated mycelia.

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“…Interestingly, the presence of truffles on herbaceous plants was hidden to researchers for a long time because the primers used to specifically detect arbuscular mycorrhizal fungi (Glomeromycota, which associate with such plants) do not detect Ascomycota. There is even the possibility that additional ectomycorrhizal species colonize the roots of nonectomycorrhizal plants (Schneider‐Maunoury et al ., ). When observing nature, we often see what we are specifically looking for: this may be one of the reasons why our understanding of fungal niches is long‐standing, but not accurate.…”

Section: Do Fungal Ecological Niches Reflect More Than Mycologist's Smentioning

confidence: 97%

“…() evidenced biotrophic colonization in 17% of species, while three species induced formation of some characteristic features of ectomycorrhizas (see the Commentary by Baldrian & Kohout, ). Truffle species of the ectomycorrhizal genus Tuber (truffles) were shown to also colonize the roots of herbaceous plants where they likely live as endophytes (Gryndler et al ., ; Schneider‐Maunoury et al ., ). Although the latter interaction is still poorly understood, it may account for a specific feature of truffles’ habitat, the brûlé.…”

Section: Do Fungal Ecological Niches Reflect More Than Mycologist's Smentioning

confidence: 99%

“…Beyond ‘the’ niche we attribute to one fungal species lie populations of individuals: dual niches, within a species, may not pertain to any individual. Some individuals may, for genetic and/or environmental reasons, be more prone to use one or another aspect of a niche, while other individuals may use the whole dual niche, as demonstrated in endophytic and ectomycorrhizal truffles (Schneider‐Maunoury et al ., ). Understanding the intraspecific dimension of niche variation is promising for a micro‐evolutionary approach, but remains a difficult, overlooked issue (Johnson et al ., ).…”

Section: Final Considerationsmentioning

confidence: 99%

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Time to re‐think fungal ecology? Fungal ecological niches are often prejudged

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Is Tuber melanosporum colonizing the roots of herbaceous, non-ectomycorrhizal plants?

Cited by 42 publications

References 62 publications

In vitro evidence of root colonization suggests ecological versatility in the genus Mycena

In vitro evidence of root colonization suggests ecological versatility in the genus Mycena

Ascoma genotyping and mating type analyses of mycorrhizas and soil mycelia of Tuber borchii in a truffle orchard established by mycelial inoculated plants

Time to re‐think fungal ecology? Fungal ecological niches are often prejudged

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