IS292: a novel insertion element from Agrobacterium

Ponsonnet, Cécile; Normand, Philippe; Pilate, Gilles; Nesme, Xavier

doi:10.1099/13500872-141-4-853

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…As is the case with relatively homologous elements found in A. xylinum, S. meliloti and A. tumefaciens (Coucheron 1993;Laberge et al 1995;Ponsonnet et al 1995), ISZm1068 was found to be present in single copy in the genomes of different Z. mobilis strains (Fig. 4), a fact that possibly indicates a lack of mobility inside the native organism.…”

Section: Discussionmentioning

confidence: 88%

“…Two types of PCR primers were designed making use of (a) homologies between the transposase coding regions, and (b) conserved IR sequences of ISs that belong to the genera of Acetobacter (Coucheron 1993;Iversen et al 1994), Agrobacterium (Machida et al 1984;Bonnard et al 1989;De Meirsman et al 1990;Paulus et al 1991;Wabiko 1992;Ponsonnet et al 1995), Sinorhizobium (Dusha et al 1987;Soto et al 1992;Selbitschka et al 1995) and Pseudomonas (Ferrante and Lessie 1991). Primers that finally resulted in the amplification of single fragments from the Z. mobilis strain CP4 genome were GT2 alone or in combination with either GT11 or GT12.…”

Section: Resultsmentioning

confidence: 99%

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IS Zm1068: an IS 5 -like insertion element from Zymomonas mobilis

Galeros

Pappas

Beletsiotis

et al. 2001

Archives of Microbiology

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A new insertion sequence, designated ISZm1068, was isolated from Zymomonas mobilis strain CP4. This element consists of 1,068 bp and contains one major ORF which shows similarities both at the nucleotide and at the amino acid sequence level with the corresponding ORFs encoding the transposases of many IS5 family elements, in particular the IS1031 group. Moreover, the Z. mobilis ORF shares the conserved N2, N3 and C1 signature motifs of the IS4 and IS5 families. Six out of seven Z. mobilis wild-type strains were shown by hybridisation to contain a single copy of the ISZm1068 element. Nucleotide sequences of the insertion elements from these strains exhibited extremely high levels of identity, varying from 94.25 to 99.25%. ISZm1068 was shown to be active in Escherichia coli cells and led to plasmid replicon fusions within the host cell. Sequence analysis of rare cointegration and resolution derivatives suggests that ISZm1068 has putative imperfect inverted repeats at its extremities of 18 bp (IR-right) and 14 bp (IR-left), and that a 3-bp (5'-TCA-3') target sequence is duplicated upon insertion.

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Section: Discussionmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

IS Zm1068: an IS 5 -like insertion element from Zymomonas mobilis

Galeros

Pappas

Beletsiotis

et al. 2001

Archives of Microbiology

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“…1 d) contains additional essential Vir operons from pTiBo542 including virA, virJ, virB, virG, virC, virD and virE , associated with plant transformation (reviewed in (Nester 2014 )). A 3.2 kb fragment including the IS292-like insertion element (Ponsonnet et al 1995 ) between virA and virJ and an 8.1 kb fragment between virJ and virB1 were deleted from the vir gene cluster in plasmid pPHP79761 (Fig. 2 ).…”

Section: Resultsmentioning

confidence: 99%

An improved ternary vector system for Agrobacterium-mediated rapid maize transformation

et al. 2018

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Key messageA simple and versatile ternary vector system that utilizes improved accessory plasmids for rapid maize transformation is described. This system facilitates high-throughput vector construction and plant transformation.AbstractThe super binary plasmid pSB1 is a mainstay of maize transformation. However, the large size of the base vector makes it challenging to clone, the process of co-integration is cumbersome and inefficient, and some Agrobacterium strains are known to give rise to spontaneous mutants resistant to tetracycline. These limitations present substantial barriers to high throughput vector construction. Here we describe a smaller, simpler and versatile ternary vector system for maize transformation that utilizes improved accessory plasmids requiring no co-integration step. In addition, the newly described accessory plasmids have restored virulence genes found to be defective in pSB1, as well as added virulence genes. Testing of different configurations of the accessory plasmids in combination with T-DNA binary vector as ternary vectors nearly doubles both the raw transformation frequency and the number of transformation events of usable quality in difficult-to-transform maize inbreds. The newly described ternary vectors enabled the development of a rapid maize transformation method for elite inbreds. This vector system facilitated screening different origins of replication on the accessory plasmid and T-DNA vector, and four combinations were identified that have high (86–103%) raw transformation frequency in an elite maize inbred.Electronic supplementary materialThe online version of this article (10.1007/s11103-018-0732-y) contains supplementary material, which is available to authorized users.

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“…C58) and CFBP 2177, respectively. pTi292: pTi2177 with the insertion element IS292 (Ponsonnet et al, 1995). Tumor indicates total numbers of isolates for each conjugated chromosome and Ti-plasmid patterns obtained in four different plots in Orléans and Peyrat-le-Château.…”

Section: Detection In Complex Environmentsmentioning

confidence: 99%