IS4 is found between eleven or twelve base pair duplications

Habermann, P.; Klaer, R.; Kühn, S.; Starlinger, Peter

doi:10.1007/bf00397237

Cited by 45 publications

(3 citation statements)

References 24 publications

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“…It has been described previously that the length of target duplication can vary for some IS elements (IS 1 : Iida and Hiestand‐Nauer, 1986; IS 4 : Mayaux et al ., 1984, Habermann et al ., 1979; IS 21 : Reimmann et al ., 1989; IS 186 : Sengstag et al ., 1986). On the contrary, a 2 bp duplication of the target sequence has been found so far in all examined IS 30 insertions (Caspers et al ., 1984; Olasz et al ., 1993; and this work).…”

Section: Resultsmentioning

confidence: 99%

Target specificity of insertion element IS30

Olasz

Kiss

König

et al. 1998

Molecular Microbiology

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SummaryThe Escherichia coli resident mobile element IS30 has pronounced target specificity. Upon transposition, the element frequently inserts exactly into the same position of a preferred target sequence. Insertion sites in phages, plasmids and in the genome of E. coli are characterized by an exceptionally long palindromic consensus sequence that provides strong specificity for IS30 insertions, despite a relatively high level of degeneracy. This 24-bp-long region alone determines the attractiveness of the target DNA and the exact position of IS30 insertion. The divergence of a target site from the consensus and the occurrence of 'nonpermitted' bases in certain positions influence the target activity. Differences in attractiveness are emphasized if two targets are present in the same replicon, as was demonstrated by quantitative analysis. In a system of competitive targets, the oligonucleotide sequence representing the consensus of genomic IS30 insertion sites proved to be the most efficient target. Having compared the known insertion sites, we suppose that IS30-like target specificity, which may represent an alternative strategy in target selection among mobile elements, is characteristic of the insertion sequences IS3, IS6 and IS21, too.

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Section: Resultsmentioning

confidence: 99%

Target specificity of insertion element IS30

Olasz

Kiss

König

et al. 1998

Molecular Microbiology

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“…There does not seem to be any correlation between the length of duplication and excision frequency. The element IS4, which generates an 11 bp or 12 bp repeat (Haberman et al, 1979), excises precisely at a frequency of 1 per lo9 cells (Pfeifer, cited by Starlinger, 1980), whereas ISl, which generates a 9 bp repeat, reverts at a frequency as high as 10e6 per cell plated (Jordan et aZ., 1968).…”

Section: Discussionmentioning

confidence: 99%

Structural and functional studies of insertion element IS200

Lam

Roth

1986

Journal of Molecular Biology

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The nucleotide sequence of the insertion element IS200 has been determined partially, including the junctions between the element and the host chromosome at the insertion site. At most, two bases (A-A) are found repeated at the junctions and could be duplications of host sequences generated by the insertion of the element. No obvious sequence repeats, either direct or inverted, have been detected between the sequences just within the two ends of the element. The element is an extremely strong block to host transcription across the insertion site. A sequence similar to known transcription termination signals was found just within the element near the right end. Removal of less than 50 base-pairs at the right end of the element abolishes the transcription block. The putative terminator sequence is located within this 50 base-pair region. Genetic studies suggest that the element contains a promoter located more than 93 base-pairs from its left end. The proposed promoter and terminator are in proper orientation to form an internal transcription unit.

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“…Therefore, the 1-bp alteration in the IS variant is not responsible for the generation of an 8-bp repeat. Among the IS elements so far studied in E. coli K-12, IS4 is the only other element known to generate target duplications of variable length (41)(42)(43). IS4 shows stringent target specificity and generates 11-, 12-, and 13-bp target duplications.…”

mentioning

confidence: 99%

Transposable element IS1 intrinsically generates target duplications of variable length.

Iida¹,

Hiestand-Nauer²,

Arber³

1985

Proc. Natl. Acad. Sci. U.S.A.

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Target duplication during transposition is one of the characteristics of mobile genetic elements. IS], a resident insertion element of Escherichia coli K-12, was known to generate a 9-base-pair target duplication, while an IS] variant, characterized by a nucleotide substitution in one of its terminal inverted repeats, was reported to duplicate 8 base pairs of its target during cointegration. We have constructed a series of transposons flanked by copies of either the normal or the variant IS]. The analysis of their transposition products revealed that transposons with normal termini as well as those with variant termini can intrinsically generate either 9-or 8-base-pair target duplications. We also observed that a normal IS) from the host chromosome generated an 8-basepair repeat. The possible relevance of the observation for the understanding of transposition processes and models to explain the variable length of target duplications are discussed.

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IS4 is found between eleven or twelve base pair duplications

Cited by 45 publications

References 24 publications

Target specificity of insertion element IS30

Target specificity of insertion element IS30

Structural and functional studies of insertion element IS200

Transposable element IS1 intrinsically generates target duplications of variable length.

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