ISCEV extended protocol for derivation and analysis of the strong flash rod-isolated ERG a-wave

Brigell, Mitchell; Jeffrey, Brett G.; Mahroo, Omar A.; Tzekov, Radouil

doi:10.1007/s10633-019-09740-4

Cited by 19 publications

(14 citation statements)

References 24 publications

(88 reference statements)

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“…Users should consult manufacturers or make appropriate measurements to establish that the correct corresponding scotopic luminance values are achieved when LEDs are used as a light source. Chromatic (non-white) flashes and backgrounds are not part of the ISCEV Standard ERG, but are used in several ISCEV extended ERG protocols [ 4 – 6 , 9 , 10 ].…”

Section: Technologymentioning

confidence: 99%

“…In addition to the ISCEV Standard ERG, extended protocols may be indicated to enhance patient management, or for detailed characterization such as functional phenotyping or clinical trials. ISCEV extended ERG protocols currently include those for the photopic negative response [ 4 ], the dark-adapted red flash ERG [ 5 ], the photopic On–Off ERG [ 6 ], stimulus–response series for light-adapted full-field ERG [ 7 ] and for the dark-adapted full-field ERG b-wave [ 8 ], the S-cone ERG [ 9 ], and for the derivation and analysis of the strong flash rod-isolated ERG a-wave [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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ISCEV Standard for full-field clinical electroretinography (2022 update)

et al. 2022

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The full-field electroretinogram (ERG) is a mass electrophysiological response to diffuse flashes of light and is used widely to assess generalized retinal function. This document, from the International Society for Clinical Electrophysiology of Vision (ISCEV), presents an updated and revised ISCEV Standard for clinical ERG testing. Minimum protocols for basic ERG stimuli, recording methods and reporting are specified, to promote consistency of methods for diagnosis, monitoring and inter-laboratory comparisons, while also responding to evolving clinical practices and technology. The main changes in this updated ISCEV Standard for clinical ERGs include specifying that ERGs may meet the Standard without mydriasis, providing stimuli adequately compensate for non-dilated pupils. There is more detail about analysis of dark-adapted oscillatory potentials (OPs) and the document format has been updated and supplementary content reduced. There is a more detailed review of the origins of the major ERG components. Several tests previously tabulated as additional ERG protocols are now cited as published ISCEV extended protocols. A non-standard abbreviated ERG protocol is described, for use when patient age, compliance or other circumstances preclude ISCEV Standard ERG testing.

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Section: Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ISCEV Standard for full-field clinical electroretinography (2022 update)

et al. 2022

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“…We calculated parameters for the rod-driven a-wave after subtracting photopically matched ERG waveforms (51). We t the following equation described by Birch & Hood to the leading edge of the rod awave (52,53):…”

Section: Rod-driven A-wave Modelmentioning

confidence: 99%

Characterization of Scotopic and Mesopic Rod Signaling Pathways in Dogs Using the On-Off Electroretinogram

Pasmanter

Petersen‐Jones

2021

Preprint

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Background: The On-Off, or long flash, full field electroretinogram (ERG) separates retinal responses to flash onset and offset. Depending on degree of dark-adaptation and stimulus strength the On and Off ERG can be shaped by rod and cone photoreceptors and postreceptoral cells, including ON and OFF bipolar cells. Interspecies differences have been shown, with predominantly positive Off-response in humans and other primates and a negative Off-response in rodents and dogs. However, the rod signaling pathways that contribute to these differential responses have not been characterized. In this study, we designed a long flash protocol in the dog that varied in background luminance and stimulus strength allowing for some rod components to be present to better characterize how rod pathways vary from scotopic to mesopic conditions.Results: With low background light the rod a-wave remains while the b-wave is significantly reduced resulting in a predominantly negative waveform in mesopic conditions. Through modeling and subtraction of the rod-driven response, we show that rod bipolar cells saturate with dimmer backgrounds than rod photoreceptors, resulting in rod hyperpolarization contributing to a large underlying negativity with mesopic backgrounds. Conclusions: Reduction in rod bipolar cell responses in mesopic conditions prior to suppression of rod photoreceptor responses may reflect the changes in signaling pathway of rod-driven responses needed to extend the range of lighting conditions over which the retina functions.

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“…ISCEV publishes and maintains other standards for clinical electrophysiological testing: specifically for the full-field ERG [2], pattern ERG [4], electrooculogram [5] and visual evoked potential [6] as well as technical and calibration guidelines for clinical electrodiagnostic testing [7] and extended protocols [8][9][10][11][12][13][14][15]. The ISCEV web site should be consulted for current updates (www.ISCEV.org/standards).…”

Section: Introductionmentioning

confidence: 99%

ISCEV standard for clinical multifocal electroretinography (mfERG) (2021 update)

et al. 2021

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The multifocal electroretinogram (mfERG) is an electrophysiological test that allows the function of multiple discrete areas of the retina to be tested simultaneously. This document, from the International Society for Clinical Electrophysiology of Vision (ISCEV), presents an updated and revised ISCEV standard for clinical mfERG and defines minimum protocols for basic clinical mfERG recording and reporting so that responses can be recognized and compared from different laboratories worldwide. The major changes compared with the previous mfERG standard relate to the minimum length of m-sequences used for recording, reporting of results and a change in document format, to be more consistent with other ISCEV standards.

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ISCEV extended protocol for derivation and analysis of the strong flash rod-isolated ERG a-wave

Cited by 19 publications

References 24 publications

ISCEV Standard for full-field clinical electroretinography (2022 update)

ISCEV Standard for full-field clinical electroretinography (2022 update)

Characterization of Scotopic and Mesopic Rod Signaling Pathways in Dogs Using the On-Off Electroretinogram

ISCEV standard for clinical multifocal electroretinography (mfERG) (2021 update)

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