Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil

Sağsöz, Nevin; Kısa, Üçler; Apan, Alparslan

doi:10.1093/humrep/17.11.2972

Cited by 72 publications

(53 citation statements)

References 38 publications

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“…In addition, I/R injuries are related to the accumulation of activated neutrophils that release ROS, such as superoxide free radical, hydrogen peroxide, hydroxyl free radical, and reactive nitrogen species [53,54]. If these free radicals are not neutralized by endogenous or exogenous antioxidant molecules, lipid peroxidation and inactivation of antioxidant enzymes will occur at the cell membrane, which, in turn, lead to damage of the cell membrane protein and capillary endothelium [19,25,27]. Medical therapies such as anti-inflammatory and antioxidant free radical scavengers directed towards the mediators responsible for ischemic damage should be used for preventing tissue damage and irreversible changes [23,55].…”

Section: Discussionmentioning

confidence: 99%

“…It is encountered as several degrees of tissue damage. The total damage that the tissue incurs is the sum of that caused by both ischemia and reperfusion [16,17,18,19]; as a result, the prevention of reperfusion injury increases the success of all kinds of treatments [16,17]. I/R injury results in an excessive production of reactive oxygen species (ROS) associated with a number of factors, including ROS and cytokines in tissues such as brain, kidney, heart, muscle, and ovaries [20,21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Nigella Sativa Reduces Tissue Damage in Rat Ovaries Subjected to Torsion and Detorsion: Oxidative Stress, Proinflammatory Response and Histopathological Evaluation

Bayır¹,

Karagöz

Karakuş³

et al. 2012

Gynecol Obstet Invest

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Background: To evaluate biochemically and histopathologically the effects of Nigella sativa (NS) in experimental ischemia and ischemia/reperfusion (I/R) injury in rat ovaries. Methods: Thirty-six female rats were divided into 6 groups: group I = sham operation; group II = 500 mg/kg NS + sham operation; group III = bilateral ovarian ischemia; group IV = 500 mg/kg NS + ischemia; group V = 3-hour period of ischemia + 3-hour reperfusion, and group VI: 3-hour period of ischemia + 500 mg/kg NS 2.5 h after the induction of ischemia + 3-hour reperfusion. At the end of ischemia, the bilateral vascular clips were removed, and 3-hour reperfusion was continued. IL-1β, IL-6, and TNF-α cytokine levels in serum, and superoxide dismutase (SOD), myeloperoxidase (MPO), glutathione (GSH), and malondialdehyde (MDA) levels were determined. Results: I/R increased the MDA level and MPO activity while significantly decreasing the SOD activity and GSH level when compared to the sham. The 500-mg/kg dose of NS before I/R reversed the trend in MDA levels, MPO activity, SOD activity, and GSH levels. Ischemia and I/R increased the serum levels of IL-1β, IL-6, and TNF-α, while the administration of NS decreased the serum levels of these cytokines. Conclusions: The administration of NS is effective in reversing tissue damage induced by ischemia and/or I/R in ovaries.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nigella Sativa Reduces Tissue Damage in Rat Ovaries Subjected to Torsion and Detorsion: Oxidative Stress, Proinflammatory Response and Histopathological Evaluation

Bayır¹,

Karagöz

Karakuş³

et al. 2012

Gynecol Obstet Invest

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“…Histological assessment was conducted under a light photomicroscope (Nikon DS-Fi2) by the same pathologist who was blinded to the study protocol. The scoring system used for histopathological evaluation of ovarian tissues was previously described by Sağsöz et al [14]. Interstitial edema, congestion (vascular dilatation), hemorrhage and loss of tissue cohesion (separation of parenchymal cells along with normal ovarian cortex and follicles) were scored from 0 to 3 (0 = no pathological finding, 1 = pathology in <33% of the section area, 2 = pathology in 33-66% and 3 = pathology in >66%) and the sum of the 4 parameters (0-12) used as the final tissue damage score.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Platelet Rich Plasma on Experimental Ischemia/Reperfusion Injury in Rat Ovary

Bakacak

Bostancı

İnanç³

et al. 2015

Gynecol Obstet Invest

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Background/Aims: Ovarian torsion is a common cause of local ischemic damage, reduced follicular activity and infertility. Platelet-rich plasma (PRP) contains growth factors with demonstrated cytoprotective properties; so we evaluated PRP efficacy in a rat ischemia/reperfusion (I/R) model. Methods: Sixty adult female Sprague-Dawley albino rats were randomly assigned to 6 groups of 8 animals each: Sham, Ischemia, I/R, Sham + PRP, I + PRP and I/R + PRP; and the remaining 12 used to prepare PRP. Ischemia groups were subjected to bilateral adnexal torsion for 3 h, while I/R and I/R + PRP groups received subsequent detorsion for 3 h. Intraperitoneal PRP was administered 30 min prior to ischemia (Ischemia + PRP) or reperfusion (I/R + PRP). Results: Total oxidant status (TOS), oxidative stress index (OSI) and total ovarian histopathological scores were higher in Ischemia and I/R groups than in the Sham group (p < 0.05). PRP decreased mean TOS, OSI and histopathological scores in I + PRP and I/R + PRP groups compared to the corresponding Ischemia and I/R groups (p < 0.001). There was a strong correlation between total histopathological score and OSI (r = 0.877, p < 0.001). Peritoneal vascular endothelial growth factor was significantly higher in PRP-treated groups than corresponding untreated groups (p < 0.05). Conclusion: PRP is effective for the prevention of ischemia and reperfusion damage in rat ovary.

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“…Few chemicals and drugs such as melatonin, CAPE, vitamin C, mannitol, verapamil, and pentoxiphylline have been studied to prevent the ischemia-reperfusion (IR) injury in ovarian torsion and detorsion in experimental models [4][5][6]. These products were found to be effective in preventing ovarian damage.…”

Section: Introductionmentioning

confidence: 99%

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

et al. 2007

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We aimed to detect the protective effect of trapidil in ischemia-reperfusion (IR) injury due to ovarian torsion and detorsion. Thirty-two pubertal New Zealand albino rabbits were used. Adnexal torsion was created by rotating the left adnexa including the tubal and ovarian vessels in a 360 degrees clockwise direction. Adnexal detorsion was done by untwisting the adnexa. In the IR group, left oopherectomy was performed after 3 h of adnexal torsion and 3 h of adnexal detorsion. In the study group, a 3-h adnexal torsion was performed and trapidil was administered intraperitoneally as a single dose of 40 mg/kg, 1 h before detorsion. The left oopherectomy was performed after a 3-h adnexal detorsion. In the sham group, sham operation was performed followed by left oopherectomy. In the control group, normal ovarian tissue was evaluated. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels of ovarian tissue were determined for each group. The values of SOD and GSH-Px activities in the IR group were significantly decreased (P < 0.05). In addition, the MDA level was significantly higher in the IR group (P < 0.01). The trapidil-administered group showed significant increase in the levels of GSH-Px (P < 0.05), catalase (P < 0.05), SOD (P < 0.05), and decreased MDA levels (P < 0.05) compared to those in the IR group. The study has shown that trapidil treatment prevents ischemia induced oxidative damage in the ovarian tissues of rabbits.

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Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil

Abstract: Vitamin C and mannitol were found to be effective in reducing ischaemia-reperfusion injury of the ovary during its early stages, but verapamil was ineffective.

Cited by 72 publications

References 38 publications

<b><i>Nigella Sativa</i></b> Reduces Tissue Damage in Rat Ovaries Subjected to Torsion and Detorsion: Oxidative Stress, Proinflammatory Response and Histopathological Evaluation

<b><i>Nigella Sativa</i></b> Reduces Tissue Damage in Rat Ovaries Subjected to Torsion and Detorsion: Oxidative Stress, Proinflammatory Response and Histopathological Evaluation

Protective Effect of Platelet Rich Plasma on Experimental Ischemia/Reperfusion Injury in Rat Ovary

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

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