Ischaemic conditioning strategies reduce ischaemia/reperfusion-induced organ injury

Pacsoo, C.; Mathew, Hans; Ma, Daqing

doi:10.1093/bja/aeu302

Cited by 35 publications

(23 citation statements)

References 161 publications

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“…In recent years, the use of baicalin sildenafil [19], resveratrol [20] and other drugs can alleviate IIRI to some extent. It is of great significance to explore the new pathogenesis of IIRI and appropriate intervention methods [21].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia–reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway

Jiang

Fan

et al. 2020

Journal of Pediatric Surgery

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Aim: To investigate the effects of hydrogen-rich saline (HRS) on intestinal epithelial tight junction (TJ) barrier in rats with intestinal ischemia-reperfusion injury (IIRI). Materials and methods: Thirty-two healthy male Sprague-Dawley (SD) rats were randomly divided into four groups (n = 8 each): Sham group, I/R group, HRS group and 4-PBA group. After 45 min of ischemia and 6 h of reperfusion, the rats were sacrificed to collect serum and ileum for detection. Hematoxylin and eosin (H&E) staining was used to observe the morphology of small intestine. The serum expression levels of intestinal fatty acid binding protein (IFABP), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were determined by enzyme linked immunosorbent assay (ELISA). Imunohistochemistry, immunofluorescence and Western blot were used to detect key proteins in intestinal epithelial TJs, ERS, and ERS-induced apoptosis, including occludin, zonula occludens-1 (ZO-1), glucose-regulated protein 78 (GRP78), X-box binding protein-1 (XBP1), C/EBP-homologous protein (CHOP) and caspase-3. Data was presented as mean ± SEM and compared using one-way ANOVA. A p-value b 0.05 was considered significant. Results: Compared with rats in the I/R group, the Chiu score of ileum damage in the HRS group and 4-PBA group were lower. The levels of serum IFABP, TNF-α, and IL-1β were statistically significant among the groups. Increased expression of TJ proteins occludin and ZO-1 by reducing various parameters of ERS and ERS-induced apoptosis evidenced by down-regulation of the protein levels of GRP78, XBP1, CHOP and caspase-3 were shown in the HRS and 4-PBA groups. Conclusion: HRS had potential protective effects on intestinal barrier in IIRI rats. This study suggested that inhibition of excessive ERS and ERS-induced apoptosis by HRS may reduce intestinal epithelial cells damage and maintain the integrity of intestinal epithelial TJ barrier in rats with IIRI.

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Section: Discussionmentioning

confidence: 99%

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia–reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway

Jiang

Fan

et al. 2020

Journal of Pediatric Surgery

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“…The mechanism of injury in many of the above situations is ischaemia reperfusion injury (IRI). [51][52][53] This is as a result of the switch of the tissue from aerobic to anaerobic respiration. Tissues are able to fully recover from short periods of ischaemia.…”

Section: Mechanisms Of Injurymentioning

confidence: 99%

Vascular surgery-related organ injury and protective strategies: update and future prospects

Yang

Fung

Pacsoo

et al. 2016

British Journal of Anaesthesia

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Whilst there has been a reduction in the prevalence of peripheral vascular disease worldwide, a significant proportion of the world's growing population is still affected by disease of the aorta, carotid, iliac and lower limb arteries. These if left untreated can result in severe morbidity and mortality. However vascular surgery, the main definitive treatment for such conditions, is associated with subsequent injury to vital organs including the kidneys, heart, brain, intestines and lungs, with a consequent increase in both morbidity and mortality. The current thinking is that the underlying mechanism of injury is direct organ ischaemia and ischaemia induced formation of free radicals, cytokine release and mitochondrial failure. Various methods to alleviate such injuries have been investigated including pre- and postconditioning strategies, pharmacological therapies including volatile anaesthetic and alpha2 adrenoceptor agonist drugs and more recently remote conditioning strategies. Although these interventions have demonstrated some reduction in the biomarkers for organ injury, attempts to translate these benefits into clinical practice have not been successful in terms of morbidity, mortality or length of hospital stay. For this reason, further research is needed in this area to facilitate the translation of the potential interventional benefits from bench to bedside.

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“…Reperfüzyonun sözü edilen olumsuz etkisi, kan akışının yeniden düzenlenmesiyle indüklenen inflamatuar tepkilerin bir sonucudur ve "İskemi/Reperfüzyon (İ/R) zedelenmesi" olarak adlandırılmaktadır. 1 Kısa süreli iskemi sonrası reperfüzyon dokuda tam iyileşme sağlayabilirken, uzun süreli iskemi sonucu oluşan doku zedelenmesine ek olarak, reperfüzyon durumu daha da kötüleştirebilir.…”

Section: İskemi̇/reperfüzyon Zedelenmesi̇unclassified

Myocardial and Renal Ischemia/Reperfusion Injury and Experimental Models: Review

Şenol¹,

Tunçtan²

2016

Turkiye Klinikleri J Pharm Sci

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Ö ÖZ ZE ET T Doku iskemisi, miyokard infarktüsü ve inme gibi klinik durumlarda ya da damar cerrahisi ve organ transplantasyonunda komplikasyon olarak ortaya çıkabilen bir durumdur. Reperfüzyon, iskemiye maruz kalan dokunun yeniden kanlanarak oksijenlenmesidir. İskemi/reperfüzyon (İ/R) zedelenmesi, kan akışının yeniden düzenlenmesiyle gelişen inflamatuar tepkilerin bir sonucudur. Erken evrede glikoliz yoluyla laktat üretimi sonucu hücre içi pH değeri düşer. Hücreleri nekroza karşı koruyan bu düşüş, reperfüzyon sıra-sında normal pH değerine yükseldiğinde iskemik hücrelerin ölümünü hızlandırır. Orta evre ise oksidatif stres ile belirgindir. Oksidatif stres belli bir eşiği aşarsa hücresel işlev bozukluğu, geri döndürülemez zedelenme ve hücre ölümü gerçekleşir. Geç evrede, inflamatuar hücreler ve adezyon molekülleri baskındır. Birçok inflamatuar molekül tarafından uyarılan nötrofil etkinliği İ/R süreci boyunca görülür. Miyokardiyal İ/R zedelenmesi miyokardiyal sersemleme (stunning), reperfüzyon aritmileri, miyositlerde nekroz ile koroner endotelyal ve mikrovasküler işlev bozukluğu gibi istenmeyen durumların ortaya çıkmasına yol açabilir. Deneysel olarak çalışılan konular miyokardiyal sersemleme, hibernasyon, reperfüzyon aritmileri ve ön koşul-lamadır. Akut renal yetmezlik (ARY) insanlarda yüksek mortalite ile ilişkili majör bir böbrek hastalığıdır. Kalp debisinin azalması, renal vasküler oklüzyon veya obstrüksiyon ile renal transplantasyon gibi durumların neden olduğu iskemi, ARY gelişmesine neden olabilir. Deneysel olarak çift taraflı (bilateral) ve tek taraflı (unilateral) renal İ/R zedelenmesi oluşturulabilmektedir. Çift taraflı iskemik ARY modeli insandaki patolojik duruma çok daha uygun olmasından dolayı yaygın olarak kullanılmaktadır. Bu çalışmada, ilaçla-rın miyokardiyal veya renal İ/R zedelenmesine bağlı olarak gelişen fizyopatolojik değişiklikler üzerindeki etkilerinin araştırılması amacıyla kullanılan deneysel modeller üzerinde durulmuştur.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : İskemi; reperfüzyon hasarı; oksidatif stres; inflamasyon; miyokardiyal reperfüzyon hasarı; akut böbrek hasarı A AB BS S T TR RA AC CT T Tissue ischemia is a condition that can occur with clinical conditions like myocardial infarction and stroke or as a complication of vascular surgery and organ transplantation. Reperfusion is reoxygenation of tissue exposed to ischemia by restoration of blood flow. Ischemia/reperfusion (I/R) injury is a consequence of inflammatory reaction induced by modulating blood flow. Intracellular pH falls as a result of lactate production through glycolysis at early stage. The decrease protecting cells against necrosis accelerates ischemic cell death at normal pH value during reperfusion. Intermediate phase is characterized by oxidative stress. If oxidative stress exceeds a certain threshold, cellular dysfunction, irreversible injury, and cell death occurs. At the late stage, inflammatory cells and adhesion molecules are dominant. Neutrophil activity stimulated by many inflammatory molecules ...

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Ischaemic conditioning strategies reduce ischaemia/reperfusion-induced organ injury

Cited by 35 publications

References 161 publications

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia–reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia–reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway

Vascular surgery-related organ injury and protective strategies: update and future prospects

Myocardial and Renal Ischemia/Reperfusion Injury and Experimental Models: Review

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