Ischemic brain cell-derived conditioned medium protects astrocytes against ischemia through GDNF/ERK/NF-kB signaling pathway

Chu, Lan-Feng; Wang, Wei-Ti; Ghanta, Vithal K.; Lin, Chin-Ho; Chiang, Y. F.; Hsueh, Chi-Mei

doi:10.1016/j.brainres.2008.08.087

Cited by 39 publications

(27 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our relatively pure cultures of primary astrocytes, durations of OGD from 30 to 120 min did not result in significant apoptosis or loss of astrocytes. Other researchers utilized 4-24 h of OGD in experiments examining the effects of OGD on astrocytes (Papadopoulos et al, 1997;Giffard et al, 2004;Chu et al, 2008;Danilov and Fiskum, 2008;Danilov et al, 2009). However, these findings do not mean that astrocytes are unaffected by shorter durations of OGD.…”

Section: Astrocytes Are More Resistant To Ogdmentioning

confidence: 99%

“…A co-culture technique was used to show that the addition of astrocytes or astrocyte conditioned medium during the recovery period protected neurons from OGD induced cell death (Griffin et al, 2005). Chu et al (2008) showed that conditioned media from ischemic neuronal cultures protected astrocytes from subsequent ischemia in a GDNF-dependent fashion. Using the co-culture technique and preparing cultures from conditional knockout mice, Vangeison et al (2008) showed that HIF1␣ loss of function in astrocytes led to greater neuronal protection against hypoxia, but loss of HIF1␣ in neurons resulted in lower neuronal viability against hypoxia.…”

Section: Astrocytes Are More Resistant To Ogdmentioning

confidence: 99%

See 1 more Smart Citation

Primary culture of cellular subtypes from postnatal mouse for in vitro studies of oxygen glucose deprivation

Jones

Novak

Elliott

2011

Journal of Neuroscience Methods

View full text Add to dashboard Cite

Section: Astrocytes Are More Resistant To Ogdmentioning

confidence: 99%

Section: Astrocytes Are More Resistant To Ogdmentioning

confidence: 99%

Primary culture of cellular subtypes from postnatal mouse for in vitro studies of oxygen glucose deprivation

Jones

Novak

Elliott

2011

Journal of Neuroscience Methods

View full text Add to dashboard Cite

“…Serum/glucose deprivation (SGD) has served as an excellent in vitro model for the understanding of the molecular mechanisms of neuronal damage during brain ischemia and for the development of neuroprotective drugs against ischemia-induced brain injury (Chu et al 2008;Hillion et al 2005). A well-defined cell system for in vitro studies of SGD-evoked neuronal injury can be provided by the rat pheochromocytoma (PC12) cell line (Woronowicz et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Nigella sativa Extract and Thymoquinone on Serum/Glucose Deprivation-Induced PC12 Cells Death

et al. 2010

View full text Add to dashboard Cite

The serum/glucose deprivation (SGD)-induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. Nigella sativa L. (family Ranunculaceae) and its active component thymoquinone (TQ) has been known as a source of antioxidants. In the present study, the protective effects of N. sativa and TQ on cell viability and reactive oxygen species (ROS) production in cultured PC12 cells were investigated under SGD conditions. PC12 cells were cultured in DMEM medium containing 10% (v/v) fetal bovine serum, 100 units/ml penicillin, and 100 microg/ml streptomycin. Cells were seeded overnight and then deprived of serum/glucose for 6 and 18 h. Cells were pretreated with different concentrations of N. sativa extract (15.62-250 microg/ml) and TQ (1.17-150 microM) for 2 h. Cell viability was quantitated by MTT assay. Intracellular ROS production was measured by flow cytometry using 2',7'-dichlorofluorescin diacetate (DCF-DA) as a probe. SGD induced significant cells toxicity after 6, 18, or 24 h (P < 0.001). Pretreatment with N. sativa (15.62-250 microg/ml) and TQ (1.17-37.5 microM) reduced SGD-induced cytotoxicity in PC12 cells after 6 and 18 h. A significant increase in intracellular ROS production was seen following SGD (P < 0.001). N. sativa (250 microg/ml, P < 0.01) and TQ (2.34, 4.68, 9.37 microM, P < 0.01) pretreatment reversed the increased ROS production following ischemic insult. The experimental results suggest that N. sativa extract and TQ protects the PC12 cells against SGD-induced cytotoxicity via antioxidant mechanisms. Our findings might raise the possibility of potential therapeutic application of N. sativa extract and TQ for managing cerebral ischemic and neurodegenerative disorders.

show abstract

“…There are pieces of evidence suggesting a protective effect in cells by NF-κB activation via ERK [118,119]. Upon cell stimulation NF-κB is translocated into the nucleus [120], where it promotes the expression of several antiapoptotic genes such as inhibitors of apoptosis proteins (IAPs) [121] and bcl-2 family members [122].…”

Section: Extracellular Signal-regulated Protein Kinases (Erk1/2)mentioning

confidence: 99%

MAP Kinases and Prostate Cancer

Rodríguez-Berriguete¹,

Fraile²,

Galvis³

et al. 2011

Prostate Cancer - From Bench to Bedside

View full text Add to dashboard Cite

The three major mitogen-activated protein kinases (MAPKs) p38, JNK, and ERK are signal transducers involved in a broad range of cell functions including survival, apoptosis, and cell differentiation. Whereas JNK and p38 have been generally linked to cell death and tumor suppression, ERK plays a prominent role in cell survival and tumor promotion, in response to a broad range of stimuli such as cytokines, growth factors, ultraviolet radiation, hypoxia, or pharmacological compounds. However, there is a growing body of evidence supporting that JNK and p38 also contribute to the development of a number of malignances. In this paper we focus on the involvement of the MAPK pathways in prostate cancer, including the less-known ERK5 pathway, as pro-or antitumor mediators, through their effects on apoptosis, survival, metastatic potential, and androgen-independent growth.

show abstract

Ischemic brain cell-derived conditioned medium protects astrocytes against ischemia through GDNF/ERK/NF-kB signaling pathway

Cited by 39 publications

References 36 publications

Primary culture of cellular subtypes from postnatal mouse for in vitro studies of oxygen glucose deprivation

Primary culture of cellular subtypes from postnatal mouse for in vitro studies of oxygen glucose deprivation

Protective Effect of Nigella sativa Extract and Thymoquinone on Serum/Glucose Deprivation-Induced PC12 Cells Death

MAP Kinases and Prostate Cancer

Contact Info

Product

Resources

About