Ischemic stroke associated with the infusion of DMSO-cryopreserved auto-PBSCs

Hematopoietic cell infusion‐related adverse events in pediatric/small recipients in a prospective/multicenter study

“…2,3 In addition, HCI itself can lead to adverse events (AEs), such as flushing, nausea and vomiting, malodor, hypotension, hypertension, allergic reactions, arrhythmia, encephalopathy, and respiratory distress. [4][5][6][7][8][9][10] AEs associated with blood transfusion, which is usually component-based therapy, have been widely and routinely surveyed. 11 In contrast, there has been less research into HCI-related AEs (HCI-AEs) in HSCT, even though HSC products contain cells of diverse lineage, maturity, and viability; plasma with various antigens, cytokines, and antibodies; dimethyl sulfoxide (DMSO) and hydroxyethyl starch (HES) in cases of cryopreserved products.…”

“…Kazuhiko Ikeda , 1,2 Hitoshi Ohto, 1,2 Minami Yamada-Fujiwara, 1,3 Yoshiki Okuyama, 1,4 Shin-ichiro Fujiwara , 1,5 Kazuo Muroi, 1,6 Takehiko Mori, 7 Kinuyo Kasama, 8 Heiwa Kanamori, 1,9 Tohru Iseki, 1,10 Tokiko Nagamura-Inoue, 1,11 Kazuaki Kameda, 12 Junya Kanda, 13 Kazuhiro Nagai, 14 Nobuharu Fujii, 15 Takashi Ashida, 16 Asao Hirose, 17 Tsutomu Takahashi, 18 Keiji Minakawa, 2 and Ryuji Tanosaki 1 BACKGROUND: Hematopoietic cell infusion-related adverse events (HCI-AEs) in hematopoietic stem cell transplantations (HSCTs) have been largely attributed to toxicity of dimethyl sulfoxide (DMSO) for cryopreservation, but HSC products also contain various cells and plasma components. Our recent prospective study of 1125 HSCT recipients revealed the highest overall HCI-AE rate in bone marrow transplantation (BMT) using fresh/noncryopreserved products, although products of peripheral blood stem cell transplantation and cord blood transplantation (CBT) are generally cryopreserved with DMSO containing smaller plasma volumes.…”

Passive immune basophil activation test for the identification of allergic episodes from various adverse events elicited by haematopoietic cell transplantation: A pilot study

“…To confirm that IgE from own basophils was replaced with that of independent individuals, we measured surface IgE expression during each step using flow cytometry (FCM). As shown in Some HC-product infusion-related allergic-AEs can be caused by DMSO and dextran used during the cryopreservation processes of HC-products [4,[6][7][8]15]. The HC sources of cases A10 and A11 were fresh BM and cryopreserved CB, respectively, and the latter contained DMSO and dextran.…”

“…Myelo-and immunesuppression result in bacterial, fungal, and viral infections. In addition, infusion of HC-product, including both cellular and plasma components, by itself can lead to adverse events (AEs) such as nausea/ vomiting, flushing, allergic reactions, hypotension, hypertension, arrhythmia, and respiratory distress [3][4][5][6][7][8][9][10][11]. Although it is important to diagnose allergic diseases by clinical symptoms even in HC infusionrelated AEs, that is not always easy.…”

Dimethyl Sulfoxide Leads to Decreased Osteogenic Differentiation of Stem Cells Derived from Gingiva via Runx2 and Collagen I Expression

“…Infusion of DMSO may lead to side effects, including nausea, vomiting, fever, anaphylaxis, and headache. 18 In this regard, it has been reported that manual single wash reduces the frequency of adverse effects, but since the procedure is time-consuming, this is only recommended for patients with high risk of DMSO toxicity. 19 Moreover, a previous method involves the use of an automatic wash method for removing dimethyl sulfoxide in autologous hematopoietic stem cell transplantation, which is reported to decrease the adverse effects related to infusion.…”