Ischemically Damaged Heart After Preservation by the Cavitary Two-layer Method as a Possible Donor in Rat Heart Transplantation

Kamoda, Yasuhisa; Fujino, Yasuhiro; Tanioka, Yasuki; Sakai, Tetsuya; Kuroda, Yoshikazu

doi:10.1016/j.healun.2007.07.037

Cited by 6 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HSPs seem to play a general role in the response of the arterial wall to stress injuries and may serve, in their function as endogenous ligands, as inducers of arteriosclerosis and native atherosclerosis by interacting with TLRs [18,19]. In our previous study, rat hearts subjected to warm ischemia showed severe I/R injury, and CTL suppressed this damage, associated with the underexpression of HSPs [13]. The present study also showed that warm ischemia caused the overexpression of HSP 60 and 70, and that CTL prevented this.…”

Section: Discussionmentioning

confidence: 91%

“…The CTL method was described previously [10,13]. Each heart graft was filled with approximately 3 mL of ice-cold UW in the lumen and completely immersed in PFC using a fine net compressor.…”

Section: Cavitary Two-layer Cold Storage Methodsmentioning

confidence: 99%

“…Expression of the HSPs was calculated as the number of stained positive cells per view under a 4003 magnification microscope. Five fields from each section were selected randomly [13,17].…”

Section: Histopathologic Studiesmentioning

confidence: 99%

“…The cavitary two-layer method (CTL), a modification of the original two-layer method, is very promising as a cold preservation technique for the heart and small intestine in animal models [10][11][12] The CTL method reportedly protected against I/R injury and suppressed HSP 60 and 70 expression [13].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prevention of Intimal Hyperplasia Employing the Cavitary Two-Layer Method in Allogeneic Rat Heart Transplantation

Fujino

Kamoda

Tanioka

et al. 2012

Journal of Surgical Research

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 91%

Section: Cavitary Two-layer Cold Storage Methodsmentioning

confidence: 99%

“…Expression of the HSPs was calculated as the number of stained positive cells per view under a 4003 magnification microscope. Five fields from each section were selected randomly [13,17].…”

Section: Histopathologic Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevention of Intimal Hyperplasia Employing the Cavitary Two-Layer Method in Allogeneic Rat Heart Transplantation

Fujino

Kamoda

Tanioka

et al. 2012

Journal of Surgical Research

Self Cite

View full text Add to dashboard Cite

“…It is well known that the initiation and progression of apoptosis need abundant ATP levels. It has been reported that the ability to re-synthesize ATP rapidly after ischemia is important in the recovery phase after heart transplantation [46]. Providing oxygen and substrate for ATP generation should extend cardiac graft survival [17].…”

Section: Discussionmentioning

confidence: 99%

Pyruvate kinase isoenzyme M2 expression correlates with survival of cardiomyocytes after allogeneic rat heterotopic heart transplantation

Shi

Yang

et al. 2015

Pathology - Research and Practice

View full text Add to dashboard Cite

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Hatayama

Inubushi

Naito

et al. 2016

Sci Rep

View full text Add to dashboard Cite

We recently succeeded in resuscitating an extracted rat heart following 24–48 hours of preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). This study aimed to examine the function of rat hearts transplanted after being preserved in the high-pressure CO and O2 gas mixture. The hearts of donor rats were preserved in a chamber filled with CO and O2 under high pressure for 24 h (CO24h) or 48 h at 4 °C. For the positive control (PC) group, hearts immediately extracted from donor rats were used for transplantation. The preserved hearts were transplanted into recipient rats by heterotopic cervical heart transplantation. CO toxicity does not affect the grafts or the recipients. Light microscopy and [18F]-fluorodeoxyglucose positron emission tomography revealed that there were no significant differences in the size of the myocardial infarction or apoptosis of myocardial cells in post-transplant hearts between the PC and CO24h groups. Furthermore, at 100 days after the transplantation, the heart rate, weight and histological staining of the post-transplanted hearts did not differ significantly between the PC and CO24h groups. These results indicate that the function of rat hearts is well preserved after 24 hours of high-pressure preservation in a CO and O2 gas mixture. Therefore, high-pressure preservation in a gas mixture can be a useful method for organ preservation.

show abstract

Ischemically Damaged Heart After Preservation by the Cavitary Two-layer Method as a Possible Donor in Rat Heart Transplantation

Cited by 6 publications

References 21 publications

Prevention of Intimal Hyperplasia Employing the Cavitary Two-Layer Method in Allogeneic Rat Heart Transplantation

Prevention of Intimal Hyperplasia Employing the Cavitary Two-Layer Method in Allogeneic Rat Heart Transplantation

Pyruvate kinase isoenzyme M2 expression correlates with survival of cardiomyocytes after allogeneic rat heterotopic heart transplantation

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Contact Info

Product

Resources

About