Iscoms Containing PurifiedQuillajaSaponins Upregulate both Th1-like and Th2-like Immune Responses

Sjölander, Anders; Land, Belinda van‘t; Bengtsson, Karin

doi:10.1006/cimm.1997.1088

Cited by 101 publications

(50 citation statements)

References 36 publications

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“…Spleen cells from mice receiving influenza-ISCOMs produced higher levels of IL-2 and IFN-␥ after restimulation than cells primed with influenza antigen in Freund's complete adjuvant [82]. Administration of OVA in ISCOMs resulted in an antigenspecific up-regulation of the secretion of IL-2 and IFN-␥ compared to OVA without adjuvant, or in aluminium hydroxide [89]. The results suggest that ISCOMs are potent inducers of murine Th1 cells, an assumption that is supported by the observation that ISCOMs up-regulate the production of IgG2a antibodies and efficiently activate CTL (see below).…”

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 95%

“…Production of IL-2 was first observed in a study by Fossum et al [86] and has been demonstrated after immunization with several antigens in ISCOMs, including influenza antigen [77,80,82,86,87], OVA [88,89], HSV type 1 glycoproteins [90], and EBV gp340 [91]. Immunization with ISCOMs containing these antigens [77,80,82,[87][88][89][90][91], or antigens from the parasites L. major [28,92,93] [76].…”

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

“…Immunization with OVA-ISCOMs or PSA-2-ISCOMs induced T cells producing significant amounts of IL-5 [28,88], whereas administration of antigens in ISCOMs has been reported to both increase [89,90,92] and decrease the production of IL-10 [77,91]. The results from several studies suggest that activation of IL-4-producing T cells by ISCOMs is low or absent, when assessed by measuring the cytokine secretion into cell culture supernatants after antigen stimulation in vitro [77,80,82,88,91,92].…”

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

“…However, when the ELISPOT technique was used to analyze T cell responses to PSA-2-ISCOMs, high numbers of cells secreting IL-4 were detected despite the fact that the corresponding culture supernatants only contained trace amounts of IL-4 [28]. Moreover, OVA-ISCOMs activated T cells to produce levels of IL-4 after restimulation comparable to those from cells primed with OVA in aluminium hydroxide, an adjuvant with a very strong capacity to induce Th2-like immune responses [89]. It thus appears that ISCOMs, at least with certain antigens, have the ability to induce T cells to secrete IL-4 but that analysis of cytokine secretion in cell culture supernatants may not reflect the response in vivo.…”

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

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ISCOMs: an adjuvant with multiple functions

Sjölander

Cox

Barr

1998

Journal of Leukocyte Biology

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158

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Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 95%

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

Section: Modulation Of T Cell Responses By Iscomsmentioning

confidence: 99%

See 2 more Smart Citations

ISCOMs: an adjuvant with multiple functions

Sjölander

Cox

Barr

1998

Journal of Leukocyte Biology

Self Cite

158

View full text Add to dashboard Cite

“…ISCOM adjuvant also induces the synthesis of proinflammatory, Th1 and Th2 cytokines (e.g. TNF , IL-6, IFN and IL-4), which modulate T cell immune responses [29,30]. Upregulation of MHC class II and co-stimulatory molecules in APC has also been demonstrated in mice [13,26].…”

Section: Introductionmentioning

confidence: 99%

Protection, systemic IFNγ, and antibody responses induced by an ISCOM-based vaccine against a recent equine influenza virus in its natural host

Paillot

Grimmett²,

Elton

et al. 2008

Vet. Res.

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-In the horse, conventional inactivated or subunit vaccines against equine influenza virus (EIV) induce a short-lived antibody-based immunity to infection. Alternative strategies of vaccination have been subsequently developed to mimic the long-term protection induced by natural infection with the virus. One of these approaches is the use of immune-stimulating complex (ISCOM)-based vaccines. ISCOM vaccines induce a strong antibody response and protection against influenza in horses, humans, and a mouse model. Cell-mediated immunity (CMI) has been demonstrated in humans and mice after ISCOM vaccination, but rarely investigated in the horse. The aim of this study was to evaluate EIV-specific immune responses after intra-muscular vaccination with an ISCOM-EIV vaccine (EQUIP F) containing both equine influenza H7N7 (A/eq/Newmarket/77) and H3N8 (A/eq/Borlänge/91 and A/eq/Kentucky/98) strains. The antibody response was measured by single radial haemolysis (SRH) assay using different H3N8 EIV strains. Stimulation of type-1 immunity was evaluated with a recently developed method that measures EIV-specific IFN synthesis by peripheral blood lymphocytes (PBL). The protective efficacy of this ISCOM-based vaccine against challenge infection with a recent equine influenza (H3N8; A/eq/South Africa/4/03) strain was also evaluated. Vaccinated ponies developed elevated levels of EIV-specific SRH antibody and increased percentage of EIV-specific IFN + PBL, whereas these responses were only detected after challenge infection in unvaccinated control ponies. Vaccinates showed minimal signs of disease and did not shed virus when challenged shortly after the second immunisation. In conclusion, evidence of type-1 immunity induced by an ISCOM-based vaccine is described for the first time in horses. equine influenza virus / vaccine / ISCOM / equine IFN gamma / immunity

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Immunological Adjuvant Effect of a Water‐Soluble Polysaccharide, CPP, from the Roots of Codonopsis pilosula on the Immune Responses to Ovalbumin in Mice

Sun

2009

Chemistry & Biodiversity

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In this study, one water-soluble polysaccharide, CPP, was purified from the root of Codonopsis pilosula. The immunomodulatory effect and the adjuvant potential of CPP on the cellular and humoral immune response of ICR mice against ovalbumin (OVA) were investigated. CPP was shown not to be lethal in vivo for mice in doses ranging from 0.5 to 4 mg. ICR Mice were immunized subcutaneously with 0.1 mg of OVA alone or with 0.1 mg of OVA dissolved in saline-containing aluminum hydroxide gel (Alum) (0.2 mg), QuilA (0.01 and 0.02 mg) or CPP (0.5, 1 or 2 mg) on days 1 and 15. Two weeks later (day 28), concanavalin A (ConA)-, lipopolysaccharide (LPS)-, and OVA-stimulated splenocyte proliferation, and OVA-specific serum antibodies were measured. CPP significantly enhanced the ConA-, LPS-, or OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 1 mg (P<0.05 or P<0.01). The OVA-specific IgG, IgG1, and IgG2b antibody levels in serum were also significantly enhanced by CPP compared with OVA control group (P<0.05 or P<0.01). The results suggest that CPP could be a safe efficacious adjuvant for use in vaccines against both pathogens and cancer.

show abstract

Iscoms Containing PurifiedQuillajaSaponins Upregulate both Th1-like and Th2-like Immune Responses

Cited by 101 publications

References 36 publications

ISCOMs: an adjuvant with multiple functions

ISCOMs: an adjuvant with multiple functions

Protection, systemic IFNγ, and antibody responses induced by an ISCOM-based vaccine against a recent equine influenza virus in its natural host

Immunological Adjuvant Effect of a Water‐Soluble Polysaccharide, CPP, from the Roots of Codonopsis pilosula on the Immune Responses to Ovalbumin in Mice

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