The real nature of the phenomenon of woman's Spirituality is the main contemporary challenge for empirical research. The literature needs many more examples of the cognitive genesis of worldviews, Spirituality and Religiousness. The first aim of this article is to present the central tenet of the Threefold Nature of Spirituality model which theoretically explains the nature of Spirituality and the theoretical relationship between beliefs (worldviews), Spirituality and Religiousness (B-S-R model). The second aim is the empirical verification of this relationship through the application of an analysis of mediation. The 308 participants were women aged 18-50 years (M = 25.25, SD = 9.42). The results obtained indicate that is a good mediator between an individual's worldview and Religiousness. Presented analysis of mediation allows us to describe the basic functioning mechanism of the spiritual sphere and the relationship between the three elements: worldview, Spirituality and Religiousness.