Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation

Liessi, Nara; Maragliano, Luca; Castagnola, Valentina; Bramini, Mattia; Benfenati, Fabio; Armirotti, Andrea

doi:10.1021/acs.analchem.0c03134

Cited by 13 publications

(15 citation statements)

References 55 publications

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“…Particularly, the authors demonstrated that isobaric labeling of proteins in the PC with subsequent analysis of MS/MS spectra of tryptic peptides represent an easy and high-throughput way to study the orientation of the proteins in the original corona. 109…”

Section: Proteomics In Nanotechnologymentioning

confidence: 99%

Nanoparticle protein corona evolution: from biological impact to biomarker discovery

2022

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Section: Proteomics In Nanotechnologymentioning

confidence: 99%

Nanoparticle protein corona evolution: from biological impact to biomarker discovery

2022

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“…Although Tf in the protein corona of CRT-MMB4@MDZ is not superior than that in the protein corona of MMB4@MDZ, CRT-MMB4@MDZ exhibited better brain-targeting effect. In recent years, researches about the spatial organization of protein coronas have come out and the randomly oriented corona proteins have been reported (Kelly et al, 2015 ; Liessi et al, 2021 ). It is reasonable to speculate that the Tf molecules bind on MMB4@MDZ with random orientation, resulted in only part of the Tf molecules orienting their TfR-binding sites to the outer-surface.…”

Section: Resultsmentioning

confidence: 99%

Brain-targeting delivery of MMB4 DMS using carrier-free nanomedicine CRT-MMB4@MDZ

Du¹,

Gao²,

Zhang³

et al. 2021

Drug Delivery

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Brain-targeting delivery of 1,1′-methylenebis[4-[(hydroxyimino)methyl]-pyridinium] dimethanesulfonate (MMB4 DMS) is limited by its hydrophilic property and chemical instability. In order to solve this problem, herein, we develop a facile protocol through combining antisolvent precipitation and emulsion-solvent evaporation method to synthesize midazolam (MDZ) coated MMB4 DMS (MMB4@MDZ) nanoparticles. The as-prepared MMB4@MDZ had a MMB4 DMS nanocrystal (MMB4-NC) core and a MDZ shell. The MDZ shell prevented the MMB4-NC core from contacting the aqueous environment, and thus, guaranteed the chemical stability of MMB4 DMS. Most charmingly, the iron mimic cyclic peptide CRTIGPSVC (CRT) was modified on MMB4@MDZ surfaces to produce CRT-MMB4@MDZ which was endowed with ability to absorb transferrin (Tf)-abundant corona. Taking advantages of the Tf-abundant corona, CRT-MMB4@MDZ achieved transferrin receptor (TfR)-mediated brain-targeting delivery. With the fascinating chemical stability and brain-targeting delivery effect, CRT-MMB4@MDZ showed great clinical transform prospect as a brand-new nanomedicine. Of particular importance, this work promised not only a core–shell carrier-free nanomedicine platform for effective delivery of unstable water-soluble drug, but also a protein corona-manipulating strategy for targeting delivery.

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“…In addition, protein corona analysis data after incubation of GBMs with human plasma can be used in enrichment analysis to identify alterations in biological processes and molecular functions. [ 184 ]…”

Section: Computational Approaches For Sbd Of Gbmsmentioning

confidence: 99%

Surface Functionalization of Graphene‐Based Materials: Biological Behavior, Toxicology, and Safe‐By‐Design Aspects

et al. 2021

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drug delivery, bioimaging probes, antibacterial agents, and tissue engineering. [2] The advantage of GBMs over other nanomaterials (NMs) in biomedical application is their high specific surface area which allows high density functionalization and drug loading on both sides of the planar structure. [3] The π-conjugated system offers high capacity for GBMs to interact with various organic compounds with aromatic structures through π-π stacking during fabrication of graphene composites or for immobilization of biomolecules such as nucleic acids, peptides, antibodies, or other therapeutic substances. [4] The same properties may also raise concerns regarding the potential risk that GBMs may cause to human health through binding of key signaling molecules for example. GBMs may interact with biomolecules, changing the structure of protein or DNA or induce oxidative damage, and so on. [5] Therefore, prior to the widespread use of GBMs, it is imperative to evaluate their potential risk to environmental and human health and to establish a proactive approach for the safe design of these materials.In a recent review article, Fadeel et al. comprehensively examined the literature on the effects of GBMs on human health and the environment and gave an overview of the state-of-the-art of safety assessment of GBMs, highlighting the importance of The increasing exploitation of graphene-based materials (GBMs) is driven by their unique properties and structures, which ignite the imagination of scientists and engineers. At the same time, the very properties that make them so useful for applications lead to growing concerns regarding their potential impacts on human health and the environment. Since GBMs are inert to reaction, various attempts of surface functionalization are made to make them reactive. Herein, surface functionalization of GBMs, including those intentionally designed for specific applications, as well as those unintentionally acquired (e.g., protein corona formation) from the environment and biota, are reviewed through the lenses of nanotoxicity and design of safe materials (safe-by-design). Uptake and toxicity of functionalized GBMs and the underlying mechanisms are discussed and linked with the surface functionalization. Computational tools that can predict the interaction of GBMs behavior with their toxicity are discussed. A concise framing of current knowledge and key features of GBMs to be controlled for safe and sustainable applications are provided for the community.

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Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation

Cited by 13 publications

References 55 publications

Nanoparticle protein corona evolution: from biological impact to biomarker discovery

Nanoparticle protein corona evolution: from biological impact to biomarker discovery

Brain-targeting delivery of MMB4 DMS using carrier-free nanomedicine CRT-MMB4@MDZ

Surface Functionalization of Graphene‐Based Materials: Biological Behavior, Toxicology, and Safe‐By‐Design Aspects

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