Isobolographic analysis of interactions – a pre-clinical perspective

Abstract

Introduction. Isobolographic analysis is the preferred method of assessment of pharmacodynamic interactions occurring among drugs administered in mixture in both pre-clinical and clinical studies. Despite its mathematical complexity, rigorous preliminary conditions and various prerequisites to be met, it assesses the pharmacodynamic interactions, classifying them as additive, antagonistic, synergistic or indifferent in nature. These interactions are usually plotted in the Cartesian system of coordinates forming isobolograms. The strength (power) of interactions is calculated and presented as an interaction index. Conclusion.This report provides basic information on the isobolographic analysis used experimentally in preclinical conditions indicating the underestimation of this valuable method in pharmacology and toxicology.