Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway

Abstract

Isochlorogenic acid A, also called 3,5-dicaffeoylquinic acid (3,5-diCQA), is a widespread phenolic compound in the plant. Recent studies have shown that it has antioxidant and anti-inflammatory activity. In addition, oxidative stress and inflammation induced by solar ultraviolet radiation is a very significant reason for skin depigmentation. Therefore, in this study, we evaluated the effect of 3,5-diCQA on B16 cells and explored its molecular mechanism. Results showed that 3,5-diCQA upregulated intracellular melanin production in a time- and dose-dependent manner. Tyrosinase (TYR) activity was also increased after treatment with 3,5-diCQA in a dose-dependent manner. Expressions of TYR, TYR-related protein1, TYR-related protein2, and microphthalmia-associated transcription factor were upregulated in a dose-dependent manner after 48 h of treatment with 3,5-diCQA. Results also showed that 3,5-diCQA promoted the phosphorylation of Akt at Thr308 and glycogen synthase kinase-3β at Ser 9. Moreover, 3,5-diCQA increased the content of β-catenin in cell cytoplasm and nucleus by reducing the content of phosphorylated β-catenin (p-β-catenin). All these results suggest that 3,5-diCQA may mediate the acceleration of melanin synthesis by the β-catenin signal pathway.

Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd

“…Our study group have focused on identifying novel compounds in Kaliziri, and have isolated 1,5-diCQA using high performance liquid chromatography (HPLC) and preparative HLPC, which may be the active ingredient ( 38 , 39 ). Previous studies suggested that methyl 3,5-dicaffeoylquinate and 3,5-dicaffeoylquinic acid, two different structural analogues of 1,5-diCQA, induce melanin synthesis ( 40 , 41 ). Therefore, we hypothesized that 1,5-diCQA may have effects on melanin synthesis.…”

“…1,5-DiCQA is a structural analogs of isochlorogenic acid A, which stimulates melanin synthesis via the Wnt signaling pathway ( 41 ). Therefore, the present study examined the effect of 1,5-diCQA on the Wnt signal pathway.…”

Hydrogen Sulfide Promotes Cell Proliferation and Melanin Synthesis in Primary Human Epidermal Melanocytes

“…e HEMs were treated with NaHS at the indicated concentration for 24 h and then incubated in normal medium for another 24 mentation diseases such as albinism and vitiligo. Several previous studies have shown some substances have a regulatory effect on melanin synthesis [21][22][23]. Compared with these agents, the therapeutic advantage of H 2 S is that it is a kind of small molecular weight, water-and lipid-soluble intrinsic gasotransmitter that can freely penetrate into the cell membrane to exert a biological effect.…”

Quantitative Determination of Marker Compounds and Fingerprint Analysis of the Seeds of Vernonia anthelmintica

“…According to the best of our knowledge, there are no previous reports on the development of a fingerprint study of V. anthelmintica herb profiles to distinguish their geographical origins in various V. anthelmintica herb-producing countries. In the previous studies about this plant, we found that three dicaffeoyl quinic acids (CQAs) play an important role in the treatment of vitiligo [ 23 , 24 ], the 3 CQAs are an isomer and hence there is also the possibility of interchanging each other, and they are high in V. anthelmintica . Therefore, the quantitative determination of the 3 CQAs as marker compounds was studied in this article, and chromatographic fingerprinting was established by combination with a basic conventional analytical, highly precise, accurate, and novel pattern recognition method (HPLC-DAD) for quality control of V. anthelmintica herbs from different countries of origin.…”