Isocitratlyase vonCandida guilliermondii, Stamm H17. III. Regelung dutch Intermediate des Alkanabbaus und allgemeines Regulationsmodell

Hildebrandt, Wulf; Weide, H.

doi:10.1002/jobm.3630140105

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…But in C. tropicalis, ICL is also inhibited by oxaloacetate, but to a lower degree (Ki = 5.0 mM) [48]. ICL is inhibited by the excess of its substrate and products (ICA, glyoxylate and succinate) [33,48,[53][54][55][56].…”

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

“…Pyruvate is also a strong inhibitor of ICL, Ki for ICL from C. guilliermondii being equal to 0.8 mM [53], and that from C. tropicalis, 5.0 mM [48]. ICL is also inhibited by TCA cycle intermediates: citrate (≥5 mM for C. tropicalis [48] and 10 mM for C. guilliermondii [53]); α-ketoglutarate (≥5 mM for C. tropicalis [48]); fumarate (9 mM for C. guilliermondii [53]) and malate 9 mM for C. guilliermondii [53]). The ICL of C. guilliermondii is strongly inhibited by 0.1 mM oxaloacetate (Ki = 0.05 mM) [53].…”

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

“…The data available in the literature for yeasts show that phosphoenolpyruvate is a noncompetitive inhibitor of ICL, the inhibitor constant Ki being different for different yeasts. For example, Ki for S. cerevisiae is equal to 1-4 mM [49], for C. guilliermondii, 1.6 mM [53], for C. tropicalis, 5 mM [48]. Pyruvate is also a strong inhibitor of ICL, Ki for ICL from C. guilliermondii being equal to 0.8 mM [53], and that from C. tropicalis, 5.0 mM [48].…”

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

“…For example, Ki for S. cerevisiae is equal to 1-4 mM [49], for C. guilliermondii, 1.6 mM [53], for C. tropicalis, 5 mM [48]. Pyruvate is also a strong inhibitor of ICL, Ki for ICL from C. guilliermondii being equal to 0.8 mM [53], and that from C. tropicalis, 5.0 mM [48]. ICL is also inhibited by TCA cycle intermediates: citrate (≥5 mM for C. tropicalis [48] and 10 mM for C. guilliermondii [53]); α-ketoglutarate (≥5 mM for C. tropicalis [48]); fumarate (9 mM for C. guilliermondii [53]) and malate 9 mM for C. guilliermondii [53]).…”

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

“…ICL is also inhibited by TCA cycle intermediates: citrate (≥5 mM for C. tropicalis [48] and 10 mM for C. guilliermondii [53]); α-ketoglutarate (≥5 mM for C. tropicalis [48]); fumarate (9 mM for C. guilliermondii [53]) and malate 9 mM for C. guilliermondii [53]). The ICL of C. guilliermondii is strongly inhibited by 0.1 mM oxaloacetate (Ki = 0.05 mM) [53]. But in C. tropicalis, ICL is also inhibited by oxaloacetate, but to a lower degree (Ki = 5.0 mM) [48].…”

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

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Effects of Medium Components on Isocitric Acid Production by Yarrowia lipolytica Yeast

et al. 2020

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The microbiological production of isocitric acid (ICA) is more preferable for its application in medicine and food, because the resulting product contains only the natural isomer—threo-DS. The aim of the present work was to study ICA production by yeast using sunflower oil as carbon source. 30 taxonomically different yeast strains were assessed for their capability for ICA production, and Y. lipolytica VKM Y-2373 was selected as a promising producer. It was found that ICA production required: the limitation of Y. lipolytica growth by nitrogen, phosphorus, sulfur or magnesium, and an addition of iron, activating aconitate hydratase, a key enzyme of isocitrate synthesis. Another regulatory approach capable to shift acid formation to a predominant ICA synthesis is the use of inhibitors (itaconic and oxalic acids), which blocks the conversion of isocitrate at the level of isocitrate lyase. It is recommended to cultivate Y. lipolytica VKM Y-2373 under nitrogen deficiency conditions with addition of 1.5 mg/L iron and 30 mM itaconic acid. Such optimized nutrition medium provides 70.6 g/L ICA with a ratio between ICA and citric acid (CA) equal 4:1, a mass yield (YICA) of 1.25 g/g and volume productivity (QICA) of 1.19 g/L·h.

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Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

Section: Inhibition Of Isocitrate Lyasementioning

confidence: 99%

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Effects of Medium Components on Isocitric Acid Production by Yarrowia lipolytica Yeast

et al. 2020

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Untersuchungen zur Regulation der Enzyme des Glyoxylatzyklus bei Saccharomycopsis lipolytica I. Einfluß der C‐Quelle auf die Aktivität von Isocitratlyase und Malatsynthase

Hönes

1983

J of Basic Microbiology

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Comperative studies on the'activities of isocitrate lyase (ICL) and malate synthase (MS) were carried out with Saccharornycopsis lipolytica incubating the yeast on media with different carbon sources. When cells were incubated in minimal medium with glucose, the activities of both enzymes were very low. I n contrast, in minimal medium with acetate enhanced enzyme activities could be demonstrated. It is probably that the synthesis of ICL is repressed in presence of glucose. Furthermore the activity of ICZ was inhibited by tricarboxylic acid cycle intermediates like succinic acid and oxalacetic acid. It was concluded that the syntheses of enzymes are derepressed. When cells of Sm. Zipolytica were incubated in minimal medium with acetate, a high enzyme activit y is evident. Synthesis of ICL on acetate was inhibited by cycloheximid and actinomycin D. The results were discussed comparing them with data obtained from other organisms.

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Characterization of isocitrate lyase from the yeast Yarrowia lipolytica

Hönes¹,

Simon²,

Weber³

1991

J. Basic Microbiol.

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The active form of isocitrate lyase (ICL) from the yeast Yarrowia lipolytica was eluted as single peak from ion exchange on DEAE-cellulose. The enzyme had a specific activity of 7.4 U/mg. Its molecular mass was estimated to be approximately 200 to 210 kDa by gel filtration chomatography on Sephadex G 200. In SDS-polyacrylamide gel electrophoresis the enzyme was characterized by an unique protein band of about 50 kDa, thus indicating that ICL is a tetramer of identical subunits. The optimum pH was 6.0 in 50mM phosphate buffer at 30°C. The K,,, value of the 35-fold purified ICL for threo-D,-isocitrate in 50 mM phosphate buffer at pH 6.0 was 0.3 mM. In regulation studies ICL was found to be noncompetitive inhibited by succinate and oxaloacetate.Antibodies against ICL were raised in rabbits. The specificity of the anti-ICL-antibodies was estimated by OUCHTERLONY tests and by a competitive ELISA on microtiter plates.

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Isocitratlyase vonCandida guilliermondii, Stamm H17. III. Regelung dutch Intermediate des Alkanabbaus und allgemeines Regulationsmodell

Cited by 6 publications

References 11 publications

Effects of Medium Components on Isocitric Acid Production by Yarrowia lipolytica Yeast

Effects of Medium Components on Isocitric Acid Production by Yarrowia lipolytica Yeast

Untersuchungen zur Regulation der Enzyme des Glyoxylatzyklus bei Saccharomycopsis lipolytica I. Einfluß der C‐Quelle auf die Aktivität von Isocitratlyase und Malatsynthase

Characterization of isocitrate lyase from the yeast Yarrowia lipolytica

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