Isoelectric focusing of catalase from acatalasemic mouse and human blood, and cultured human skin fibroblasts

Ogata, Masana; Satoh, Y.

doi:10.1002/elps.1150090305

Cited by 14 publications

(3 citation statements)

References 17 publications

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“…This catalase is rapidly degraded and has a different electrophoretic mobility [4, 51. The Japanese type of this syndrome indicated no substantial difference between normal and acatalasemic erythrocytes in the charge of catalase protein or molecular size [6,71. A guanine to adenine substitution at the fifth position of intron four (a splice site mutation) seems to be responsible for the defective catalase synthesis in the Japanese type of acatalasemia.…”

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confidence: 99%

Genetic heterogeneity in acatalasemia

Góth

Páy

1996

Electrophoresis

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203 bp long products containing exon 4 and its junctions from the catalase gene were generated by polymerase chain reaction (PCR). These products were analyzed by single strand conformational polymorphism (SSCP), hetero-duplex formation and nucleotide sequencing. No polymorphism was detected when the Hungarian acatalasemic sisters, their family members and normocatalasemic controls were analyzed. Sequence analyses did not show the G to A point mutation at position 5 of intron 4. This splicing mutation characterizes the Japanese-type of acatalasemia.

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confidence: 99%

Genetic heterogeneity in acatalasemia

Góth

Páy

1996

Electrophoresis

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“…The residual catalase of the Japanese type does not differ from the normal enzyme with respect to isoelectric point, heat stabil ity and molecular weight [4,5]. On the other hand, in Swiss-type acatalasemia the resid ual catalase activity exhibits a higher mobil-We presented a preliminary report on 2 acatalasémie sisters in Hungary [13].…”

Section: Introductionmentioning

confidence: 99%

“…Catalase was detected [4] by immersing the gel plate in 1 % H2C>2 for 5 s, followed by transfer into a vessel containing 50 ml of a 1 % KI solution, adjusted to pH 3.0 with 7% acetic acid. The staining of starch proceeded slowly until all but the area of catalase activity was stained.…”

Section: Isoelectric Focusing Of Catalasementioning

confidence: 99%

Characterization of Acatalasemia Detected in Two Hungarian Sisters

Góth¹

1992

Enzyme

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Acatalasemia was detected in 2 sisters of a Hungarian family. The pedigree of the family showed hypocatalasemia in the children of the patients and in 1 of their brothers, while the other members of the family had normal blood catalase activity. The biochemical characterization (catalase activity, electrophoretic migration, isoelectric point and enzyme stability) of the blood as well as tissue catalase of the acatalasémie patients yielded a catalase form which did not differ from normal.

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Characterization of human residual catalase of an acatalasemic patient by isoelectric focusing and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis followed by electrophoretic blotting and immunodetection

1989

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Isoelectric points and subunit sizes of catalases in human blood and human cultured skin fibroblasts from acatalasemic and normal subjects were analyzed by isoelectric focusing in agarose gel and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively, followed by electroblotting to polyvinylidene difluoride membranes for immunodetection. The results indicated that the isoelectric point of residual catalase in the C fraction prepared from acatalasemic erythrocytes was identical with that of catalase prepared from normal erythrocytes. The residual catalase in homogenates of acatalasemic cultured skin fibroblasts also reacted with anticatalase rabbit serum and had an isoelectric point identical with that of normal catalase. Subunit sizes of normal and acatalasemic catalases in the C fractions of erythrocytes were also found to be identical on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by electroblotting and immunoenzymatic amplification. The results indicated no substantial difference in molecular size and charge of catalase proteins between normal and acatalasemic erythrocytes and fibroblasts.

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Isoelectric focusing of catalase from acatalasemic mouse and human blood, and cultured human skin fibroblasts

Cited by 14 publications

References 17 publications

Genetic heterogeneity in acatalasemia

Genetic heterogeneity in acatalasemia

Characterization of Acatalasemia Detected in Two Hungarian Sisters

Characterization of human residual catalase of an acatalasemic patient by isoelectric focusing and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis followed by electrophoretic blotting and immunodetection

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