Isoenzymatic variability of cassava accessions from different regions in Brazil

Cabral, Betânia Lúcia Rocha; Souza, Joelson Araújo de; Ando, Akihiko; Veasey, Elizabeth Ann; Cardoso, E. M. R.

doi:10.1590/s0103-90162002000300017

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…A similar result (64.4% variation within households) was obtained by Veasey et al (2007) when studying morphological traits of the same accessions. This same pattern of a greater portion of variation being due to differences between accessions within households has also been reported for cassava (Sambatti et al, 2000), as well as within groups or regions (Faraldo et al, 2000;Cabral et al, 2002). Other studies with sweet potatoes using AMOVA have also shown higher diversity within rather than between regions, which could, on a much smaller scale, be compared to the higher diversity found within rather than between households and communities.…”

Section: Genetic Structure and Analysis Of Molecular Variancesupporting

confidence: 74%

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

et al. 2008

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We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses showed a Jaccard's index from 0.3 to 1.0, indicating high genetic and intravarietal diversity. Accessions from all 19 communities were not spatially structured (r = 0.15, p < 0.054), with AMOVA indicating that most of the variability (58.2%) was distributed within households and only 18.1% of the variability was distributed between households within communities. The outcrossing mating system of sweet potato, and anthropic factors such as selection of different varieties and their maintenance within household small plots and home gardens, as well as an extensive exchange system between agriculturists, may all be contributing to these results.

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Section: Genetic Structure and Analysis Of Molecular Variancesupporting

confidence: 74%

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

et al. 2008

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“…This result was also shown within cassava populations in Costa Rica (Zaldivar et al 2004) and Africa (Raji et al 2009), within regions or cluster groups of cassava (Mühlen et al 2000;Cabral et al 2002;Lokko et al 2006;Siqueira et al 2009), as well as within households with sweet potato (Veasey et al 2008). Peroni et al (2007) obtained values indicating that despite the higher diversity of the sweet varieties of cassava, in comparison with the bitter ones, the divergence among the two groups was low, however, significant, with most genetic diversity concentrated within groups.…”

Section: Genetic Structuresupporting

confidence: 52%

Microsatellite Polymorphisms in Cassava Landraces from the Cerrado Biome, Mato Grosso do Sul, Brazil

Siqueira

Pinheiro²,

Borges³

et al. 2010

Biochem Genet

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Using nine microsatellite loci, we investigated genetic structure and diversity in 83 Brazilian cassava accessions, including several landraces, in the Cerrado biome in Mato Grosso do Sul, Brazil. All nine loci were polymorphic, averaging 6.00 alleles per locus. Treating each of seven municipalities as a cassava group or population, they averaged 3.5 alleles per locus, with 97% polymorphic loci, high values for observed heterozygosity (0.32) and gene diversity (0.56). Total genetic variability was high (0.668), and most of this genetic variability was concentrated within municipalities (0.577). Cluster and structure analyses divided accessions into two major clusters or populations (K = 2). Also, a significant genetic versus geographic correlation was found (r = 0.4567; P < 0.0260). Migratory routes in the Cerrado are considered main contributors to the region's high cassava diversity and spatial genetic structure, amplifying interactions between traditional farmers and the evolutionary dynamics of this crop.

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“…In order to understand the important role of traditional farmers in maintaining and even amplifying genetic diversity in cassava landraces in Brazil and other countries, various studies have been undertaken with isozyme markers ( Sambatti et al , 2000 ; Faraldo et al , 2000 ; Cabral et al , 2002 ; Resende et al , 2004 ), randomly amplified polymorphic DNA (RAPD) markers ( Colombo et al , 1998 ; Carvalho and Schaal, 2001 ; Zacarias et al , 2004 ), and microsatellites or simple sequence repeats (SSR) ( Mühlen et al , 2000 ; Carvalho and Schaal, 2001 ; Peroni, 2007 ), the latter being an appropriate marker for the detection of genetic polymorphisms, widely used to characterize genetic diversity in traditional crops ( Mühlen et al , 2000 ; Fregene et al , 2003 ; Elias et al , 2004 ; Veasey et al , 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of cassava ( Manihot esculenta ) landraces in Brazil assessed with simple sequence repeats

Siqueira¹,

Queiroz-Silva²,

Bressan³

et al. 2009

Genet. Mol. Biol.

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Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava (Manihot esculenta) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves (HS = 0.52). Cluster analysis and principal component based scatter plotting showed greater similarity among landraces from São Paulo, Mato Grosso do Sul and Amazonas, whereas those from Minas Gerais were clustered into a sub-group within this group. The plants from Mato Grosso, mostly collected in the municipality of General Carneiro, provided the highest differentiation. The migration of human populations is one among the possible reasons for this closer resemblance or greater disparity among plants from the various regions.

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Isoenzymatic variability of cassava accessions from different regions in Brazil

Cited by 14 publications

References 10 publications

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

Microsatellite Polymorphisms in Cassava Landraces from the Cerrado Biome, Mato Grosso do Sul, Brazil

Genetic characterization of cassava ( Manihot esculenta ) landraces in Brazil assessed with simple sequence repeats

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