Isoenzyme characterisation of Trichomonas vaginalis.

Soliman, M A; Ackers, J. P.; Catterall, R. D.

doi:10.1136/sti.58.4.250

Cited by 11 publications

(9 citation statements)

References 15 publications

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“…Humoral and local antibody responses have indicated strain differences and 2-8 serotypes of T.vaginalis have been demonstrated (Soliman et al 1982), but specific immunogenic differences among strains have not been well characterised. MAbs were also used to classify strains on the basis of surface antigen profile, but this has not met with success (Alderete et al 1987), probably owing to the ability of the parasite to undergo protein and epitope phenotypic variation.…”

Section: Discussionmentioning

confidence: 97%

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kaur

Gupta

et al. 2001

Parasitol Res

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Trichomoniasis caused by Trichomonas vaginalis may lead to either a complete absence of symptoms or to severe inflammatory manifestations in infected women. Studies of the role of immune responses in the pathogenesis and varied symptomatology of this disease are lacking. Mice may prove useful as an experimental model for intravaginal trichomoniasis in developing an understanding of the role of local immune responses in the pathogenesis and varied symptomatology of this disease. The present study reports the levels of anti-Trichomonas IgA antibodies in serum and vaginal washes, and T-cell subtype and cytokine profile in vaginal cervical tissues of mice infected intravaginally with T. vaginalis isolates from 15 symptomatic and 15 asymptomatic women. It also correlates the responses with symptomatology of the patients. Successful intravaginal infection was established by inoculating T. vaginalis in BALB/c mice preinoculated with Lactobacillus acidophilus and pretreated with oestradiol. A significant increase in specific IgA antibody levels was detected with enzyme-linked immunosorbent assay in vaginal secretions and serum samples collected on the 7th post-infection day from animals infected with isolates from asymptomatic women when compared with mice infected with isolates from symptomatic women. T-cell subset analysis showed significant differences, with increased CD4+ T-cell count in animals infected with isolates from asymptomatic women compared with animals infected using isolates from symptomatic women. No difference in CD8+ T cells was observed between the two groups. Cytokine profile revealed significantly higher (P < 0.001) production of gamma-IFN and IL-2 in mice infected with asymptomatic isolates compared with animals infected with symptomatic isolates, using T. vaginalis crude antigen extract and nonspecific mitogen (ConA) as stimulants for vaginal cervical lymphocytes. However, no difference in IL-4 levels was observed in the two groups of animals. In contrast, significant increase in tumour necrosis factor (TNF-alpha) levels was observed in animals infected with asymptomatic isolates compared with those infected with isolates from symptomatic women and controls, thereby indicating that TNF-alpha may play an important role in the inflammatory response to trichomoniasis. The study further suggests that specific IgA antibodies might help to protect asymptomatic individuals from severe infection and T-lymphocytes may play an important function in the eradication of the parasite. The cytokine profile indicated the involvement of Th-1 like responses in mice infected with asymptomatic isolates, compared with those infected with symptomatic isolates.

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Section: Discussionmentioning

confidence: 97%

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kaur

Gupta

et al. 2001

Parasitol Res

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“…14,[27][28][29] Isoenzyme characterization based on the electrophoretic patterns of four enzymes, classified 32 Trichomonas isolates into five groups, 28 and 63 isolates into 15 groups. 29 The reaction-profiles of 88 isolates to a battery of monoclonal antibodies identified 11 distinct patterns; some groups contained isolates from five disparate geographic sites and one geographic site contained representatives of all 11 groups.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing of Trichomonas vaginalis isolates by HSP70 restriction fragment length polymorphism.

Stiles

Shah²,

Li³

et al. 2000

Am J Trop Med Hyg

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Abstract. Subtyping isolates of Trichomonas vaginalis is an essential tool for understanding the epidemiology of this common sexually-transmitted disease. Restriction fragment length polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA was used in the molecular typing of Trichomonas isolates. Analysis of five American Type Culture Collection (ATCC) reference strains and 31 Jackson, Mississippi, isolates from six male and 21 female patients, revealed 10 distinct RFLP pattern subtypes of Trichomonas. The subtypes were temporally stable and cosmopolitan. The RFLP profiles seen in Maryland, Ohio, Massachusetts, and New York ATCC strains were identical to those of some Mississippi isolates, even though the samples were isolated 10-35 years apart. There was no correlation between metronidazole resistance and RFLP subtype with resistant isolates from eight patients distributed among six different subtypes.

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“…Both parasite and host factors seem to play a role in leading to symptomatic infection. The isoenzyme analysis (Soliman et al 1982;Vohra et al 1991), antigenic structure of the parasite (Alderete 1983) and restriction fragment length polymorphism analysis (Sapru et al 1994) have not been able to clearly differentiate isolates from symptomatic and asymptomatic women. However, other multiple factors such as adhesive ability, cell detaching factors, poreforming proteins and soluble factors like haemolysins and extracellular proteinases are recognised virulence markers (Fiori et al 1999).…”

Section: Introductionmentioning

confidence: 99%

In vitro haemolytic and cytotoxic activity of soluble extract antigen of T. vaginalis isolates from symptomatic and asymptomatic women

et al. 2008

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Human Trichomonas vaginalis infection ranges from asymptomatic to mild, moderate or severe clinical manifestations. The reasons for diverse symptomatology have been found to vary in several attributes and both parasite and host factors appear to play a role in the pathogenesis. The present study reports the in vitro haemolytic and cytotoxic activity of crude soluble extract (CSE) antigen of T. vaginalis isolates from 15 symptomatic and 15 asymptomatic women. The haemolytic activity following the interaction of CSE antigen with human erythrocytes and cytotoxic activity by adding CSE antigen to normal human vaginal epithelial cells was significantly higher with the use of CSE antigen of isolates from symptomatic women as compared to those from asymptomatic women. Furthermore, cytotoxic effect was found to be pH dependent. The study demonstrates, for the first time, the significant effect of parasite antigen of isolates from symptomatic women as compared to those from asymptomatic women in inducing haemolytic and cytotoxic effect and supports the earlier report that contact-independent mechanism(s) may be playing a role in establishing symptomatic trichomoniasis.

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Isoenzyme characterisation of Trichomonas vaginalis.

Cited by 11 publications

References 15 publications

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Molecular typing of Trichomonas vaginalis isolates by HSP70 restriction fragment length polymorphism.

In vitro haemolytic and cytotoxic activity of soluble extract antigen of T. vaginalis isolates from symptomatic and asymptomatic women

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