Isoenzymes of malic dehydrogenase, glutamic oxaloacetic transaminase and lactic dehydrogenase in serum in diseases of the liver

Schmidt, E.; Schmidt, W; Otto, Paulo Alberto

doi:10.1016/0009-8981(67)90067-8

Cited by 67 publications

(15 citation statements)

References 8 publications

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“…2 partate aminotransferases (EC 2.6.1.1), (2)(3)(4)(5)(6), which differ in their amino acid composition and in their immunochemical and kinetics properties (7)(8)(9). As early äs 1967, Schmidt et al (10) started to consider the possible clinical usefulness of measuring of these isoenzymes. Until recently, measurement of these isoenzymes was not widely applied for clinical purposes, chiefly because the methods (electrophoresis (2-4), column chromatography (10, 11)) were tedious, slow, imprecise and inaccurate, and not adaptable for routine use.…”

Section: Introductiohmentioning

confidence: 99%

Clinical and Diagnostic Significance of Aspartate Aminotransferase Isoenzymes in Sera of Patients with Liver Diseases

Panteghini¹,

Malchiodi²,

Calarco³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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Summary: Serum aspartate aminotransferase isoenzymes were measured in 123 hospital patients with various liver diseases, using a new and simple immunochemical method.Our findings show the usefulness of this determination in estimating the severity of hepatic damage, especially if accompanied by the measurement of other mitochondrial enzymes/During hepatic damage, the cytoplasmic isoenzyme is found in greater quantities than the mitochondrial isoenzyme, but the level of the latter increases to a greater extent in acute liver diseases. Moreover, the values of mitochondrial aspartate aminotransferase activity and the ratio of mitochondrial to total aspartate aminotransferase in alcoholic hepatitis are higher than expected if so-called hepatic enzymes commonly measured in serum are considered. These results indicate that there is significant mitochondrial damage in alcoholic hepatitis. Klinische und diagnostische Bedeutung von

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Section: Introductiohmentioning

confidence: 99%

Clinical and Diagnostic Significance of Aspartate Aminotransferase Isoenzymes in Sera of Patients with Liver Diseases

Panteghini¹,

Malchiodi²,

Calarco³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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“…After myocardial infarction, liver damage and several in vitro injury conditions, mitochondrial isoenzymes are not released to the same extent äs their cytosolic isoenzymes (43,63,(79)(80)(81)(82).…”

Section: Ultra-turrax) and Fractionated Extraction (Bücher)mentioning

confidence: 99%

Catalytic Enzyme Activity Concentration in Tissues of Man, Dog, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology, III. Communication

Lindena¹,

Sommerfeld²,

Höpfel³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary:The catalytic aetivity of up to fifteen enzymes was investigated in the liver, heart, skeletal muscle, kidney (medulla, cortex), brain, hing, duodenum, spieen and pancreas from man and animals. Human specimens were obtained from autopsies and immediately post-mortem from dogs, rabbits, guinea pigs, rats and mice. The differences between our results and previous reports of considerably lower activities for structural enzymes (e. g. creatine kinase) and for enzymes partly of mitochondrial origin (e. g. glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase), is attributed to our use of a detergent extraction technique. The superiority of the detergent technique with regard to enzyme yield is exemplified by a comparison of various methods of extraction in rat liver, heart and skeletal muscle. Use of standardized assays allows a qualitative inter-species comparison of results. The influence of autolysis on catalytic aetivity of human autopsies is considered of minor importance. Katalytische Enzymaktivitätskonzentration in Geweben von

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“…The effects of sarcotoxin have been not fully elucidated as yet (Schmidt 1967). Regarding the fact that liver sinusoids possess no basal membranes, its presence in liver parenchyma may enhance the enzyme transport from cells into the blood.…”

Section: Discussionmentioning

confidence: 99%

Catalytic Activity of Selected Serum Enzymes in Experimental Sarcocystosis in Calves

Nevole¹,

Malota²,

Koudela³

1986

Acta Vet. Brno

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Isoenzymes of malic dehydrogenase, glutamic oxaloacetic transaminase and lactic dehydrogenase in serum in diseases of the liver

Cited by 67 publications

References 8 publications

Clinical and Diagnostic Significance of Aspartate Aminotransferase Isoenzymes in Sera of Patients with Liver Diseases

Clinical and Diagnostic Significance of Aspartate Aminotransferase Isoenzymes in Sera of Patients with Liver Diseases

Catalytic Enzyme Activity Concentration in Tissues of Man, Dog, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology, III. Communication

Catalytic Activity of Selected Serum Enzymes in Experimental Sarcocystosis in Calves

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