Isoflavone supplementation reduces DNA oxidative damage and increases O-β-N-acetyl-d-glucosaminidase activity in healthy women

Erba, Daniela; Casiraghi, Maria Cristina; Conesa, Cristina Martínez; Goi, G.; Massaccesi, Luca

doi:10.1016/j.nutres.2012.03.007

Cited by 27 publications

(14 citation statements)

References 49 publications

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“…Thus, the production of ROS is inhibited, and there is a consequent inhibition of cell death. In another study, reduction in DNA oxidative damage and increase in O-b-N-acetyl-D-glucosaminidase enzymatic activity were observed in healthy women whose diets were supplemented with isoflavones (Erba et al, 2012). Ding & Liu (2011) suggested that genistein reduces the levels of oxidative stress and increases antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase, possibly via the MAPK (ERK1/2) pathway.…”

Section: Discussionmentioning

confidence: 97%

Soy isoflavones have antimutagenic activity on DNA damage induced by the antileishmanial Glucantime (meglumine antimoniate)

Cantanhêde

Almeida

Soares

et al. 2014

Drug and Chemical Toxicology

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Isoflavones are phytoestrogens reported to be potent antioxidant agents. In contrast, the antileishmanial meglumine antimoniate has mutagenic activities. This study evaluated the ability of soy isoflavones to reduce DNA damage induced by meglumine antimoniate. Antimutagenic effects (by micronucleus test) were tested using Swiss mice divided into seven groups treated with meglumine antimoniate (425 mg/kg bw pentavalent antimony); cyclophosphamide (50 mg/kg bw); water (negative control); single isoflavones dose (1.6 mg/kg bw), and three groups received one dose of isoflavones via gavage (0.4 mg/kg bw, 0.8 mg/kg bw or 1.6 mg/kg bw) plus meglumine antimoniate via intraperitoneal, simultaneously. To evaluate antigenotoxicity (by Comet assay), each group with 10 animals received the above-mentioned control doses; single dose of isoflavones 0.8 mg/kg bw, and three groups received isoflavones (0.8 mg/kg bw) by gavage along with intraperitoneal meglumine antimoniate, which were treated with isoflavones 24 h before or after receiving meglumine antimoniate (pre-treatment and post-treatment, respectively) or simultaneously. Cells were harvested 24 h after the treatment, and the data were evaluated by ANOVA followed by Tukey's test (p < 0.05). The data from the simultaneous treatment by micronucleus test revealed that isoflavones (0.4 and 0.8 mg/kg) were able to reverse the mutagenic effect of Glucantime. Moreover, all regimes of the treatment with 0.8 mg/kg bw dose were able to reduce the genotoxicity caused by meglumine antimoniate. It is suggested that the protective effect of isoflavones against DNA damage is related to their ability to reduce oxidative stress caused by the trivalent Sb(III) metabolite of meglumine antimoniate.

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Section: Discussionmentioning

confidence: 97%

Soy isoflavones have antimutagenic activity on DNA damage induced by the antileishmanial Glucantime (meglumine antimoniate)

Cantanhêde

Almeida

Soares

et al. 2014

Drug and Chemical Toxicology

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“…In rats feeding a high fat diet, it inhibits inflammation and progression of NAFLD, by activating JNK and inhibiting NF-κB, as well as TNF-α and IL-6 secretion [231]. In postmenopausal women, genistein supplementation (>1 mg/day) decreases BMI, body fat mass, waist size, and increases blood HDL [232,233]; accordingly, six months isoflavone supplementation (80 mg daily) in healthy women decreases DNA oxidative damage and increases plasma TAC [234]. One serving/day of isoflavone-enriched pasta (33 mg total isoflavones), for eight weeks, increases plasma TAC and GSH levels, while decreasing oxLDL and isoprostane 8- iso -PGF 2α , in overweight diabetic subjects [235].…”

Section: Potential Strategies To Reduce Oxidative Stress In Obesitymentioning

confidence: 99%

Obesity-Associated Oxidative Stress: Strategies Finalized to Improve Redox State

Savini

Catani

Evangelista

et al. 2013

IJMS

422

310

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Obesity represents a major risk factor for a plethora of severe diseases, including diabetes, cardiovascular disease, non-alcoholic fatty liver disease, and cancer. It is often accompanied by an increased risk of mortality and, in the case of non-fatal health problems, the quality of life is impaired because of associated conditions, including sleep apnea, respiratory problems, osteoarthritis, and infertility. Recent evidence suggests that oxidative stress may be the mechanistic link between obesity and related complications. In obese patients, antioxidant defenses are lower than normal weight counterparts and their levels inversely correlate with central adiposity; obesity is also characterized by enhanced levels of reactive oxygen or nitrogen species. Inadequacy of antioxidant defenses probably relies on different factors: obese individuals may have a lower intake of antioxidant- and phytochemical-rich foods, such as fruits, vegetables, and legumes; otherwise, consumption of antioxidant nutrients is normal, but obese individuals may have an increased utilization of these molecules, likewise to that reported in diabetic patients and smokers. Also inadequate physical activity may account for a decreased antioxidant state. In this review, we describe current concepts in the meaning of obesity as a state of chronic oxidative stress and the potential interventions to improve redox balance.

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“…This analytical approach is useful as prognostic index of plasmatic peroxidation risk in patients with oxidative pathologies (such as diabetes, cancer, hypertension, Down syndrome, and chronic renal failure) [13, 40] and is an efficient tool to monitor the effects of antioxidant treatments [25]. …”

Section: Discussionmentioning

confidence: 99%

“…Therefore we measured the above-mentioned enzyme activities both in membrane and cytosol of erythrocytes of ED patients to evaluate their possible involvement in ED and their possible use to mark oxidative stress and to monitor the patients that undergo antioxidant treatment [25]. …”

Section: Introductionmentioning

confidence: 99%

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

et al. 2014

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Oxidative stress (OS) and production of NO, by endothelium nitric oxide synthetase (eNOS), are involved in the pathophysiology of erectile dysfunction (ED). Moreover, OS induces modifications of the physicochemical properties of erythrocyte (RBC) plasma membranes and of the enzyme content of the same membranes. Due to their role in signalling early membrane alterations in OS-related pathologies, several plasma membrane and cytosolic glycohydrolases of human RBC have been proposed as new markers of cellular OS. In RBC, NOS can be activated and deactivated by phosphorylation/glycosylation. In this regulatory mechanism O-β-N-AcetylGlucosaminidase is a key enzyme. Cellular levels of O-GlcNAcylated proteins are related to OS; consequently dysfunctional eNOS O-GlcNAcylation seems to have a crucial role in ED. To elucidate the possible association between RBC glycohydrolases and OS, plasma hydroperoxides and antioxidant total defenses (Lag-time), cytosolic O-β-N-AcetylGlucosaminidase, cytosolic and membrane Hexosaminidase, membrane β-D-Glucuronidase, and α-D-Glucosidase have been studied in 39 ED patients and 30 controls. In ED subjects hydroperoxides and plasma membrane glycohydrolases activities are significantly increased whereas Lag-time values and cytosolic glycohydrolases activities are significantly decreased. These data confirm the strong OS status in ED patients, the role of the studied glycohydrolases as early OS biomarker and suggest their possible use as specific marker of ED patients, particularly in those undergoing nutritional/pharmacological antioxidant therapy.

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Isoflavone supplementation reduces DNA oxidative damage and increases O-β-N-acetyl-d-glucosaminidase activity in healthy women

Cited by 27 publications

References 49 publications

Soy isoflavones have antimutagenic activity on DNA damage induced by the antileishmanial Glucantime (meglumine antimoniate)

Soy isoflavones have antimutagenic activity on DNA damage induced by the antileishmanial Glucantime (meglumine antimoniate)

Obesity-Associated Oxidative Stress: Strategies Finalized to Improve Redox State

Levels of Human Erythrocyte Membrane-Bound and Cytosolic Glycohydrolases Are Associated with Oxidative Stress in Erectile Dysfunction Patients

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