Isoflavones Found in Korean Soybean Paste as 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Inhibitors

Sung, Jang Hoon; Choi, Soo Jung; Lee, Soon Won; Park, Kwan Hwa; Moon, Tae Wha

doi:10.1271/bbb.68.1051

Cited by 39 publications

(27 citation statements)

References 43 publications

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“…The concentrations of the isoflavones, genistein, daidzein and genistin, in the lupin protein were analyzed by an HPLC method. 25) In brief, lupin protein To analyze the amino acid concentrations in the diet, samples were oxidized and then hydrolysed with 6 M HCl. 26) Separation and quantification of the amino acids were performed by ion-exchange chromatography after post-column conversion in an amino acid analyzer (Biotronic LC 3000, Eppendorf, Hamburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Differing Effect of Protein Isolates from Different Cultivars of Blue Lupin on Plasma Lipoproteins of Hypercholesterolemic Rats

Bettzieche

Brandsch

Schmidt

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Differing Effect of Protein Isolates from Different Cultivars of Blue Lupin on Plasma Lipoproteins of Hypercholesterolemic Rats

Bettzieche

Brandsch

Schmidt

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activities were measured according to the method of Sung et al [31]. As the blank test, 600 ll sodium phosphate buffer (pH 6.8) containing 400 mM NADPH, 200 mM NaCl, 2 mM EDTA, and 20 mM dithiothreitol was added to 300 ll microsome fraction.…”

Section: Preparation Of Analytical Samples For Lipid Level Determinationmentioning

confidence: 99%

Effect of extracts from narezushi, a fermented mackerel product, on cholesterol metabolism in Wistar rats

Itou

Akahane

2010

Fish Sci

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Narezushi extract was separated into peptide and nonpeptide fractions by ion-exchange column chromatography. The narezushi extract and fractions were administered to rats in a diet enriched with lipid and cholesterol for 30 days. In the narezushi extract and nonpeptide fraction groups, increases in triglyceride, total cholesterol, and low-density lipoprotein-cholesterol levels in the plasma and accumulation of total lipids and triglyceride in the liver were suppressed, while both lipid and cholesterol fecal excretion were increased. In the peptide fraction group, these effects were also observed, except for the suppressing effect on liver lipid accumulation. Narezushi extract administration tended to increase fecal bile acids and promoted the activity of cholesterol 7a-hydroxylase, the rate-limiting enzyme in the synthesis of bile acid from cholesterol in the liver. However, the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in the cholesterol synthesis system in the liver, decreased due to regulation by the feedback of lipid transportation from diet to the liver. These results suggest that both the increase in cholesterol and bile acid fecal excretion and the promotion of cholesterol 7a-hydroxylase activities are related to the hypocholesterolemic effects of narezushi extract. Amino acids and organic acids, which are abundantly contained in the nonpeptide fraction, seemed to have more intensive hypocholesterolemic effects than peptides existing in the peptide fraction.

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“…Concentrations of the isoflavones genistein, daidzein, and genistin in the lupin protein were analyzed by an HPLC method [26]. In brief, lupin protein was extracted with a mixture of methanol and dichlormethane (1:1 v/v).…”

Section: Preparation and Characterization Of The Dietary Proteinsmentioning

confidence: 99%

Lupin protein acts hypocholesterolemic and increases milk fat content in lactating rats by influencing the expression of genes involved in cholesterol homeostasis and triglyceride synthesis

Bettzieche

Brandsch

Eder

et al. 2009

Molecular Nutrition Food Res

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Lupin protein has been shown to reduce triglyceride and cholesterol concentrations in plasma of hypercholesterolemic growing and adult rats. However, the effect of lupin protein on lipid metabolism during pregnancy and lactation is unknown. Female rats were assigned to two groups and were fed a hypercholesterolemic diet containing either 200 g/kg lupin protein or casein during pregnancy until day 18 of lactation. Dams fed lupin protein had lower triglyceride concentrations in plasma (-55%) and higher triglyceride concentrations in liver (>2-fold) and milk (+81%) than dams fed casein (p < 0.05). The concentration of cholesterol in plasma, VLDL, LDL, and liver was markedly lower and the concentration of HDL cholesterol was higher in rats fed lupin protein than in rats fed casein (p < 0.05). Lupin protein induced a 2.6-fold increase of hepatic LDL receptor concentration compared to casein (p < 0.05), down-regulated genes involved in fatty acid oxidation in the liver, and up-regulated lipogenic genes in the mammary gland (p < 0.05). This study shows that lupin protein increases milk fat content and strongly modifies triglyceride and cholesterol metabolism by influencing the transcription levels of genes involved in fatty acid oxidation and synthesis and cholesterol homeostasis.

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Isoflavones Found in Korean Soybean Paste as 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Inhibitors

Cited by 39 publications

References 43 publications

Differing Effect of Protein Isolates from Different Cultivars of Blue Lupin on Plasma Lipoproteins of Hypercholesterolemic Rats

Differing Effect of Protein Isolates from Different Cultivars of Blue Lupin on Plasma Lipoproteins of Hypercholesterolemic Rats

Effect of extracts from narezushi, a fermented mackerel product, on cholesterol metabolism in Wistar rats

Lupin protein acts hypocholesterolemic and increases milk fat content in lactating rats by influencing the expression of genes involved in cholesterol homeostasis and triglyceride synthesis

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