Isoflavones in Soy-Based Infant Formulas

Murphy, Patricia A.; Song, Tongtong; Buseman, Gwen; Barua, Kobita

doi:10.1021/jf970590t

Cited by 165 publications

(116 citation statements)

References 19 publications

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“…The results are reported as the molar sums of genistein, daidzein, and glycitein multiplied by their respective aglycone molecular weight. Isoflavone determinations were carried out, having coefficients of variation ranging between 2 and 8% for the three major isoflavones [10].…”

Section: Methodsmentioning

confidence: 99%

Soy Milk with a High Glycitein Content Does Not Reduce Low-Density Lipoprotein Cholesterolemia in Type II Hypercholesterolemic Patients

Sirtori¹,

Bosisio²,

Pazzucconi³

et al. 2002

Ann Nutr Metab

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In order to evaluate acceptability and effectiveness of a partial addition of soy protein to the daily diet in well-established type II hypercholesterolemic individuals, a double-blind study was carried out with a soy milk providing 25 g/day of protein versus an identically formulated cow’s milk. Twenty patients with type II hypercholesterolemia, 4 males and 16 females, age range 38–76 years, all with cholesterol levels >7 mmol/l and low-density lipoprotein cholesterol <5.5 mmol/l, were selected. Significant triglyceride elevations (WHO Fredrickson type IIB) were present in 4 patients, and the body mass index was 24.2 ± 3.47 kg/m². Different from prior studies, either with isoflavone-free products or with a moderately isoflavone-rich milk, the milk in the present study did not reduce total and low-density lipoprotein cholesterolemia. A detailed analysis of the composition showed significant differences in the isoflavone content versus products used in prior studies with a positive outcome. Soy milk isoflavones were, in fact, characterized by a high glycitein content and a low genistein/daidzein ratio. Glycitein is a minor component in most soy products, and its role in cholesterol regulation is unclear. In view of the high interest in the use of soy products for the prevention of coronary diseases, the metabolic behavior of different isoflavones in man should be better characterized, and the role of isoflavone composition of soy products given for the control of cholesterolemia needs further clarification.

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Section: Methodsmentioning

confidence: 99%

Soy Milk with a High Glycitein Content Does Not Reduce Low-Density Lipoprotein Cholesterolemia in Type II Hypercholesterolemic Patients

Sirtori¹,

Bosisio²,

Pazzucconi³

et al. 2002

Ann Nutr Metab

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“…The method of Murphy et al (1997) was used for isoflavone extraction and high performance liquid chromatography (HPLC) assays. A 2-g sample of dry, powdered leaf material from each of three replications of each of 12 cultivars, three wounding treatments and two experiments (total of 216 samples) was placed in a 125-mL screw-top Erlenmeyer flask and 20 mL of acetonitrile, 2 mL of 0.1 N HCl, and 7 mL of water were added.…”

Section: Extraction Of Isoflavones and Hplc Assaysmentioning

confidence: 99%

Effects of wounding and inoculation with Sclerotinia sclerotiorum on isoflavone concentrations in soybean

Wegulo¹,

Yang²,

Martinson³

et al. 2005

Can. J. Plant Sci.

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. 2005. Effects of wounding and inoculation with Sclerotinia sclerotiorum on isoflavone concentrations in soybean. Can. J. Plant Sci. 85: 749-760. Isoflavones play an important role in the defense response of soybean to pathogen attack. They are involved in nodulation of legumes and are associated with human health benefits including the prevention of heart disease and cancers. Concentrations of the isoflavones daidzein, genistein, and glycitein, the glucoside conjugates daidzin, genistin and glycitin, the acetyl glucoside conjugates acetyldaidzin, acetylgenistin, and acetylglycitin, and the malonyl glucoside conjugates malonyldaidzin, malonylgenistin, and malonylglycitin were determined in ovendried leaves of 12 soybean cultivars whose stems were (i) non-wounded, non-inoculated (NWNI), (ii) wounded, non-inoculated (WNI), and (iii) wounded and inoculated (WI) with mycelia of Sclerotinia sclerotiorum, the causal fungus of Sclerotinia stem rot of soybean. There were significant differences (P ≤ 0.05) among cultivars in concentrations of isoflavones and their conjugates in all wounding treatments. Concentrations of the aglycones daidzein and genistein were higher (P ≤ 0.05) in WI than in WNI and NWNI plants in all cultivars. Glycitein and its conjugates were detected only in some cultivars in much lower concentrations than daidzein and genistein and their conjugates. Concentrations of total daidzein in the 12 cultivars ranged from 68 to 491, 174 to 781, and 282 to 553 µg g -1 dry weight in NWNI, WNI, and WI plants, respectively. Concentrations of total genistein ranged from 128 to 427, 290 to 840, and 296 to 759 µg g -1 dry weight in NWNI, WNI, and WI plants, respectively. Concentrations of total glycitein ranged from 0 to 44, 0 to 13, and 0 to 24 µg g -1 dry weight in NWNI, WNI, and WI plants, respectively. In NWNI plants, the cultivar Corsoy 79 ranked in the top two (rank 1 = highest concentration, rank 12 = lowest concentration) in concentrations of daidzein and genistein and their conjugates except genistin (ninth rank). In WNI plants, Parker ranked first in concentrations of all conjugates of daidzein and genistein whereas Corsoy 79 consistently ranked in the top four in concentrations of the two isoflavones and their conjugates. In WI plants, Parker ranked first in concentrations of all conjugates of daidzein and genistein except acetyldaidzin (second rank), S19-90 ranked in the top five in concentrations of all conjugates of daidzein and genistein, whereas Corsoy 79 ranked in the top five in concentrations of daidzein and genistein and all of their conjugates. The results from this study suggest that soybean cultivars differ in concentrations of constitutive or induced isoflavones and in their ability to accumulate isoflavones following wounding and/or infection by S. sclerotiorum. For personal use only.

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“…Four random urine samples (about one sample per week) per cycle were selected and tested for urinary isoflavone levels to determine compliance during the soy intervention period and avoidance of these foods during baseline and recovery (urinary isoflavone analysis was conducted by Drs Patricia Murphy and Suzanne Hendrich, Iowa State University). To determine both food and urinary isoflavone levels, we used a high-performance liquid chromatography (HPLC) quantitation method, developed by these investigators which hydrolyses isoflavone metabolites and extracts and quantifies isoflavone aglycones Xu et al, 1994;Murphy et al, 1997).…”

Section: Analysis Of Isoflavone Levels In Foods and Urinesmentioning

confidence: 99%

Effects of soy foods on ovarian function in premenopausal women

Stanczyk

Hendrich

et al. 2000

Br J Cancer

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It has been proposed that the high intake of soy foods among Asians may partly explain their lower rates of breast cancer, perhaps by lowering endogenous oestrogen levels, although this has been inadequately studied. Twenty healthy cycling premenopausal women (ten Asians and ten non-Asians) participated in a 7-month soy intervention study which was designed to investigate the effect of supplementation on ovarian function. Asian soy foods (tofu, soymilk, green soybean peas) in the amount of approximately 32 mg of isoflavones per day were added to the women's diets for three menstrual cycles. The women's baseline (two cycles) serum hormone levels were compared to levels during soy intervention (three cycles) and levels after intervention (two cycles). During the entire study period, subjects provided almost daily overnight urine samples and blood specimens during specified days of their menstrual cycles. The day of urinary luteinizing hormone (LH) peak was used as a marker for the day of ovulation. Knowledge of day of ovulation allowed comparison of hormone measurements at baseline to those obtained during intervention and recovery cycles with standardization of day of cycle. Soy intervention was associated with a statistically significant reduction in serum luteal oestradiol level (–9.3%, P < 0.05), but there were no significant changes in follicular phase oestradiol, follicular or luteal phase progesterone, sex hormone-binding globulin or menstrual cycle length. This significant reduction in luteal phase oestradiol was, however, observed only among Asian (–17.4%) but not among non-Asian (–1.2%) participants; urinary excretion of isoflavones was higher among Asians than non-Asians (29.2 vs 17.1 μmol day −1 , P = 0.16) during the intervention period. Thus, supplementation using traditional soy foods reduced serum oestradiol levels among Asian participants in this study. Differences in the type of soy products (i.e. traditional soy foods versus soy protein products), amount of isoflavones, and race/ethnicity of participants may have contributed to the divergent results. Larger soy intervention studies designed specifically to include participants of different race/ethnicities and using both traditional soy foods and soy protein products providing comparable doses of isoflavones are needed to definitively determine the effect of soy on ovarian function. © 2000 Cancer Research Campaign

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Isoflavones in Soy-Based Infant Formulas

Cited by 165 publications

References 19 publications

Soy Milk with a High Glycitein Content Does Not Reduce Low-Density Lipoprotein Cholesterolemia in Type II Hypercholesterolemic Patients

Soy Milk with a High Glycitein Content Does Not Reduce Low-Density Lipoprotein Cholesterolemia in Type II Hypercholesterolemic Patients

Effects of wounding and inoculation with Sclerotinia sclerotiorum on isoflavone concentrations in soybean

Effects of soy foods on ovarian function in premenopausal women

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