Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization

Abstract

Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.

Polymeric Coatings for Drug Delivery by Medical Devices

“…Exceptional cytocompatibility was revealed on human-derived macrophages, fibroblasts and keratinocytes. The findings show that PLGA/IBUP coatings have a promising potential for surface modification of medical devices such as metallic implants and wound dressings Valentina Grumezescu et al [110] study reports on the deposition of polyvinylpyrrolidone/ antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as antiadhesion barrier coatings, on biomedical surfaces for better resistance to microbial colonization. The thin films were analyzed by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy.…”

Organic Thin Films Deposited by Matrix-Assisted Pulsed Laser Evaporation (MAPLE) for Photovoltaic Cell Applications: A Review

“…Scheme 1 presents the laser-based deposition techniques derived from PLD and their main features. The organic and hybrid layers deposited using MAPLE were usually applied in the biomedical area as antimicrobial coatings [73][74][75][76][77], bioactive coatings [78], tissue regeneration systems [79,80], bone regeneration systems [81], drug delivery systems [82][83][84], etc. However, the potential applications of the MAPLE deposited layers in other fields concerning organic photovoltaic cells [38,40,70,[85][86][87][88], hybrid photovoltaic cells [39,89,90], polymer light emitting diodes [91,92], antireflective coatings [93], photo-responsive coatings [82], non-linear optical materials [94][95][96], transparent supercapacitor electrodes [97] and sensing materials for various gases [98][99][100][101][102][103][104] has also been envisaged.…”

Bioactive Coatings Loaded with Osteogenic Protein for Metallic Implants

“…Special attention was oriented towards the latter category, given the enhanced physicochemical properties and boosted biofunctional performance of laser-textured [22][23][24] and coated [25,26] metallic implants. Out of all these techniques, the matrix-assisted pulsed laser evaporation (MAPLE) is known to have some advantages, such as the possibility to synthesize homogeneous and uniform nanosized or nanostructured coatings, as well as composite or hybrid coatings containing organic substances, such as polymers [27][28][29] and biomolecules [30,31].…”