Isoflavonoids and Phytoestrogens from Pueraria candollei var. mirifica Related with Appropriate Ratios of Ethanol Extraction

Peerakam, Nichakan; Sirisa-ard, Panee; Huy, Nguyen Quoc; Ơn, Trần Văn; Long, Phung Tuan; Intharuksa, Aekkhaluck

doi:10.14233/ajchem.2018.21440

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…These plants were extracted using ethanol, which provided a high extraction efficiency of PM isoflavonoids. 17 Extraction was performed using sonication (37 kHz) for 30 min at room temperature. The supernatant (extract) was collected after centrifugation at 8000 rpm for 5 min.…”

Section: Sample Preparationmentioning

confidence: 99%

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Phaisan

Yusakul

Nuntawong

et al. 2020

Phytochemical Analysis

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Introduction: The plant Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham (PM), known by its common Thai name as white Kwao Krua, is sometimes misidentified because it presents similar botanical characteristics to those of Butea superba (red Kwao Krua). The phytochemicals in PM are phytoestrogens in the class of isoflavonoids, but Butea superba contains flavonoids that exhibit androgenic and antiestrogen effects.Objectives: This research aims to develop a simple analytical method for identification and to differentiate PM from red Kwao Krua and other Pueraria species.Methods: A gold nanoparticle-based immunochromatographic assay (ICA) was developed for the detection of kwakhurin (Kwa), a unique compound found in PM. The parameters, including sensitivity, accuracy, precision, and specificity, were validated. All samples were analyzed using ICA and high-performance liquid chromatography with UV detector (HPLC-UV). The results of the two methods were compared for consistency checking. Results:The cutoff limit of Kwa detection was 160 ng/mL, which was lower than in the HPLC-UV method. The repeatability and reproducibility of the ICA preparation and assembly showed high precision. The cross-reactivity to related isoflavonoids was less than 0.32%, which implied high specificity of the ICA for Kwa. Moreover, false-positive and false-negative results from other plant extracts were not observed. Conclusion:The developed ICA is applicable for distinguishing PM from red Kwao Krua and other Pueraria species. This simple analytical method can be applied for the identification of raw PM materials in the industrial and agricultural sectors.

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Section: Sample Preparationmentioning

confidence: 99%

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Phaisan

Yusakul

Nuntawong

et al. 2020

Phytochemical Analysis

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“…For this reason, this plant has been widely investigated for its constituents and pharmacological activities. Peerakam et al [8] found that the 95% ethanol extract of this plant contained puerarin (1), as a major compound, and other isoflavonoids, such as daidzin, daidzein, genistin, and genistein, in addition to the phytoestrogens miroestrol and deoxymiroestrol. Suthon et al [9] evaluated the anti-osteoporotic effects of the crude extract of P. candollei var.…”

Section: Introductionmentioning

confidence: 99%

Effects of Puerarin on the Ovariectomy-Induced Depressive-Like Behavior in ICR Mice and Its Possible Mechanism of Action

et al. 2019

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Daily treatment of ovariectomized (OVX) ICR mice with puerarin, a glycosyl isoflavone isolated from the root bark of Pueraria candollei var. mirifica, and 17β-estradiol attenuated ovariectomy-induced depression-like behavior, as indicated by a decrease in immobility times in the tail suspension test (TST) and the forced swimming test (FST), an increase in the uterine weight and volume, a decrease in serum corticosterone levels, and dose-dependently normalized the downregulated transcription of the brain-derived neurotrophic factor (BDNF) and estrogen receptor (Erβ and Erα) mRNAs. Like 17β-estradiol, puerarin also inhibited ovariectomy-induced suppression of neurogenesis in the dentate gyrus of the hippocampus (increased the number of doublecortin (DCX)-immunosuppressive cells). These results suggest that puerarin exerts antidepressant-like effects in OVX animals, possibly by attenuating the OVX-induced hyperactivation of the HPA axis and/or normalizing the downregulated transcription of BDNF and ER mRNA in the brain.Molecules 2019, 24, 4569 2 of 15 depression. These symptoms can be reversed by supplementation with estrogen. A body of evidence suggests that such estrogen replacement therapy (ERT) can exert anxiolytic and antidepressant effects in menopausal females by modulating the hypothalamic-pituitary-adrenal (HPA) axis functions [3,4]. However, the drawback of ERT is its adverse effects, such as an increased risk of breast and endometrial cancers, as well as thromboembolic events [5,6]. Consequently, the long-term usage of ERT to ameliorate emotional and cognitive deficits has an obvious limitation in women. Thus, the search for new phytoestrogens, with a weak effect on reproductive organs, from natural sources continues to be an important field of research.Pueraria candollei var. mirifica is a medicinal plant used in Thai traditional medicine for its rejuvenating and estrogenic effects [7]. For this reason, this plant has been widely investigated for its constituents and pharmacological activities. Peerakam et al. [8] found that the 95% ethanol extract of this plant contained puerarin (1), as a major compound, and other isoflavonoids, such as daidzin, daidzein, genistin, and genistein, in addition to the phytoestrogens miroestrol and deoxymiroestrol. Suthon et al.[9] evaluated the anti-osteoporotic effects of the crude extract of P. candollei var. mirifica and puerarin (1) in ovariectomized (OVX) mice and found that only the crude extract at 50 mg/kg (body weight) per day caused a significant increase in the trabecular bone mineral densities of tibia metaphysis when compared to the control group. On the other hand, the pharmacological activities of puerarin (1) (Figure 1), a major isoflavonoid from P. candollei var. mirifica, have been extensively investigated. Puerarin (1) has been widely used in the treatment of diabetes, dyslipidemia, liver fibrosis, cardiovascular diseases, neurotoxicity, and Alzheimer's disease [10]. This compound was also found to exhibit various protective effects against fever, hyper...

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“…CAE produced a nonsignificant difference in the yield of total MI and DMI as compared to those produced by absolute ethanol. Thus, the process enhanced the DZe and GTe contents without (Peerakam et al 2018). The resultant extracts with 95% ethanol comprise predominantly DZ and GT rather than their aglycones after 7 days of maceration.…”

Section: Efficacy Of Caementioning

confidence: 94%

“…Usually, PMI, MI, and DMI extraction from PM is effective with 95% ethanol maceration (Peerakam et al 2018 ). Although bioethanol is a relatively safe and green solvent for herbal extract preparation, ethanol must be removed from the extract, which requires energy, time, and labor.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents

Makkliang

Siriwarin

Yusakul

et al. 2021

Bioresour. Bioprocess.

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The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus limiting their health benefits. Cellulolytic enzyme-assisted extraction (CAE) potentially solves this issue; however, solvent extraction requires recovery of the hydrophobic products. Here, we established the simultaneous transformation and extraction of PMIs using cellulolytic enzymes and natural deep eutectic solvents (NADESs). The NADES compositions were optimized to allow the use of NADESs as CAE media, and the extraction parameters were optimized using response surface methodology (RSM). The optimal conditions were 14.7% (v/v) choline chloride:propylene glycol (1:2 mol ratio, ChCl:PG) at 56.1 °C for the cellulolytic enzyme (262 mU/mL) reaction in which daidzin and genistin were extracted and wholly transformed to their aglycones daidzein and genistein. The extraction of PMIs using ChCl:PG is more efficient than that using conventional solvents; additionally, biocompatible ChCl:PG enhances cellulolytic enzyme activity, catalyzing the transformation of PMIs into compounds with higher estrogenicity and absorbability.

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Isoflavonoids and Phytoestrogens from Pueraria candollei var. mirifica Related with Appropriate Ratios of Ethanol Extraction

Cited by 7 publications

References 13 publications

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Effects of Puerarin on the Ovariectomy-Induced Depressive-Like Behavior in ICR Mice and Its Possible Mechanism of Action

Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents

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