Isoflavonoids of the Leguminosae

Veitch, Nigel C.

doi:10.1039/c3np70024k

Cited by 115 publications

(67 citation statements)

References 204 publications

(350 reference statements)

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“…Daidzein and genistein appear to share common enzymes in the subsequent derivative biosynthetic steps of the phenylpropanoid pathway (Jung et al, 2000;Dhaubhadel et al, 2003). The biosynthesis pathway of glycitein is poorly understood and likely harbors a few unique enzymes (Dhaubhadel et al, 2003;Veitch, 2007). Our correlation coefficient analysis on content of isoflavones showed no statistically significant correlation between the glycitein content and the other isoflavone contents, which generally supports this biochemical observation.…”

Section: Discussionsupporting

confidence: 89%

“…These results indicated that many minor QTL were not detected in this study due to the broad effect of the environment on isoflavone accumulation in soybean seeds. Seed isoflavone content in the soybean is governed by complex biochemical interactions that make it difficult to predict the actions of individual genes (Veitch, 2007). Since specific pathway branches control the synthesis of different isoflavone derivatives, the dissection of QTL for isoflavone aglycones or total isoflavone content into the individual components is desirable in order to gain more information regarding which QTL may involve global pathway genes and which QTL might prove more specific for individual compounds.…”

Section: Discussionmentioning

confidence: 99%

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Novel major quantitative trait loci regulating the content of isoflavone in soybean seeds

et al. 2011

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Despite their medicinal, pharmaceutical, and nutritional importance of isoflavones, the genetic basis controlling the amounts of isoflavones in soybean seeds is still not well understood. The main obstacle is the great variability in the content of isoflavone in seeds harvested from different environments. In this study, quantitative trait loci (QTL) for the content of different isoflavones including daidzein, genistein, and glycitein were investigated in a population of recombinant inbred lines derived from the cross of "Hwangkeum" (Glycine max) by "IT182932" (Glycine soja). Seeds analyzed were harvested in three different experimental environments. QTL analyses for isoflavone content were conducted by composite interval mapping across a genomewide genetic map. Two major QTL were mapped to soybean chromosomes 5 and 8, which were designated QDZGT1 and QDZGT2, respectively. Both loci have not been previously reported in other isoflavone sources. The results from this study will be useful in cloning genes that can control the contents of isoflavones in soybean and for the development of soybean lines containing a high or low isoflavone content.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Novel major quantitative trait loci regulating the content of isoflavone in soybean seeds

et al. 2011

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“…The flavones (apigenin derivatives, luteolin, and luteolin derivatives) and isoflavones (daidzein, genistein, and formononetin) were detected in relatively higher concentrations in Fabaceae than in the other two families. Flavones and isoflavones were also reported from Bauhinia L. species, Genista tinctoria and Lespedeza maximowiczii (also included in present study), thus validating our results (Tuczkiewica et al 2004;Veitch 2013;Farag et al 2015;Kim et al 2015). In general, the present work indicates that Cornaceae, Fabaceae, and Rosaceae show clear metabolite groupings as indicated through the multivariate statistical analyses.…”

Section: Plant Cell Repsupporting

confidence: 89%

Metabolite fingerprinting, pathway analyses, and bioactivity correlations for plant species belonging to the Cornaceae, Fabaceae, and Rosaceae families

et al. 2016

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A multi-parallel approach gauging the mass spectrometry-based metabolite fingerprinting coupled with bioactivity and pathway evaluations could serve as an efficacious tool for inferring plant taxonomic orders. Thirty-four species from three plant families, namely Cornaceae (7), Fabaceae (9), and Rosaceae (18) were subjected to metabolite profiling using gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS) and ultrahigh performance liquid chromatography-linear trap quadrupole-ion trap-mass spectrometry (UHPLC-LTQ-IT-MS/MS), followed by multivariate analyses to determine the metabolites characteristic of these families. The partial least squares discriminant analysis (PLS-DA) revealed the distinct clustering pattern of metabolites for each family. The pathway analysis further highlighted the relatively higher proportions of flavonols and ellagitannins in the Cornaceae family than in the other two families. Higher levels of phenolic acids and flavan-3-ols were observed among species from the Rosaceae family, while amino acids, flavones, and isoflavones were more abundant among the Fabaceae family members. The antioxidant activities of plant extracts were measured using ABTS, DPPH, and FRAP assays, and indicated that extracts from the Rosaceae family had the highest activity, followed by those from Cornaceae and Fabaceae. The correlation map analysis positively links the proportional concentration of metabolites with their relative antioxidant activities, particularly in Cornaceae and Rosaceae. This work highlights the pre-eminence of the multi-parallel approach involving metabolite profiling and bioactivity evaluations coupled with metabolic pathways as an efficient methodology for the evaluation of plant phylogenies.

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“…1 (Dewick 1982). Around 840 new isoflavonoids have been reported over last 15 years indicating the increasing interest in search for the natural isoflavonoids (Sharma and Ramawat 2013;Veitch 2013). The aim of this article is to provide an insight into the steps involved and the processes used for the extraction, identification and characterization of isoflavonoids from plant sources.…”

Section: Introductionmentioning

confidence: 97%

“…The enzymes that catalyses the substitution reactions are often encoded by large gene families, which can be recognized in expressed sequence tag (EST) and genome data sets through family-specific conserved sequence motifs (Dixon and Pasinetti 2010). In comparison to the flavonoids (2-phenylchromans), which are found widely in higher plants, the isoflavonoids appear in a limited occurrence of taxon, probably owing to the limited occurrence of IFS enzyme, being confined essentially to the subfamily Papilionoideae (Lotoideae) of the leguminosae (Klejdus et al 2005a;Veitch 2007Veitch , 2009). There are however, occasional examples of their occurrence in the subfamily Caesalpinioidae, and in other families (Rosaceae, Moraceae, Amaranthaceae, Podocarpaceae, Chenopodiaceae, Cupressaceae, Iridaceae, Myristicaceae and Stemonaceae) together with some reports of their isolation from microbial sources (Dewick 1982;Reynaud et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry

et al. 2015

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Isoflavonoids are the biologically active secondary metabolites of plants that are being used for several health promoting and restoring effects mediated through different pathways. Isoflavonoids are structurally similar to estrogens due to which also known as phytoestrogens and have shown potent estrogenic and anti-estrogenic activity. Association with large biological activity lead to the need of rapid, sensitive and precise quantitation of different isoflavonoids in different plant extracts, food materials and biological matrices as the biological activities mainly depends on the quantities and nature of isoflavonoids present in them. The characterisation, standardisation and quantification of herbal extracts or food products require techniques that are highly selective, sensitive and also provide mass measurement precisions and structural information. Liquid chromatography with tandem mass spectroscopy has made it possible to analyse and characterize several constituents and their metabolites in a single run along with high selectivity and sensitivity. In this review, we have summarised the application of LC-MS/MS for the identification and quantification of isoflavonoids reported for various plant extracts and food products along with their general extraction procedures and factors affecting extraction providing a view towards the conditions used for their analysis. The most suitable and widely acceptable extraction solvent system for the isoflavonoids is methanol or ethanol in combination with water ranging from 40 to 60 % organic solvent based on the type of tissues and the isoflavonoids to be extracted by different extraction techniques. ESI ionization with Q-TOF MS was the most useful detection system for the characterisation and quantification of the diverse isoflavonoids with molecular insights.

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Isoflavonoids of the Leguminosae

Cited by 115 publications

References 204 publications

Novel major quantitative trait loci regulating the content of isoflavone in soybean seeds

Novel major quantitative trait loci regulating the content of isoflavone in soybean seeds

Metabolite fingerprinting, pathway analyses, and bioactivity correlations for plant species belonging to the Cornaceae, Fabaceae, and Rosaceae families

Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry

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