Isoform identification and characterization of Art v 3, the lipid-transfer protein of mugwort pollen

Gadermaier, Gabriele; Harrer, Andrea; Girbl, Tamara; Palazzo, Paola; Himly, Martin; Vogel, Lothar; Briza, Peter; Mari, Adriano; Ferreira, Fátima

doi:10.1016/j.molimm.2009.03.021

Cited by 44 publications

(54 citation statements)

References 36 publications

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“…An association between LTP-mediated food allergy and pollinosis has been described in the Mediterranean area mainly with pollen LTP from plane tree (Pla a 3) [7,12,[22][23][24] and mugwort (Art v 3) [25][26][27][28][29][30][31]. The high prevalence of sensitization to these pollens in our patients-76% and 60%, respectivelysupports the hypothesis of LTP and food pollen syndrome in our area [3,28].…”

Section: Discussionsupporting

confidence: 75%

Lettuce Allergy Is a Lipid Transfer Syndrome-Related Food Allergy With a High Risk of Severe Reactions

Muñoz-García¹,

Luengo²,

Moreno-Pérez³

et al. 2017

J Investig Allergol Clin Immunol

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Background and Objectives: Lipid transfer protein (LTP) sensitization is the most common cause of food allergy in the Mediterranean area, with peach allergy acting as the primary sensitizer in most cases. Lettuce has been described as a common offending food in patients with LTP syndrome. The aim of the study was to investigate the frequency and clinical expression of LTP syndrome in a sample of lettuceallergic patients. Methods: We determined specific IgE to Pru p 3 and lettuce in a sample of 30 patients with a diagnosis of lettuce allergy. Symptoms elicited by other LTP-containing plant-derived foods and the presence of cofactors were assessed. Results: The clinical symptoms of lettuce allergy were frequently severe, with 18 of the 30 patients experiencing anaphylaxis. All the patients had allergic reactions to other plant foods. Cofactors were involved in the clinical reactions of 13 of the 30 patients. Sensitization to pollens was found in 90% of patients. Conclusions: Lettuce allergy is found not as an isolated condition but in the context of LTP syndrome and it is characterized by severe reactions and frequent cofactor association.

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Section: Discussionsupporting

confidence: 75%

Lettuce Allergy Is a Lipid Transfer Syndrome-Related Food Allergy With a High Risk of Severe Reactions

Muñoz-García¹,

Luengo²,

Moreno-Pérez³

et al. 2017

J Investig Allergol Clin Immunol

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“…Thus, it might be more common with Art v 3 sensitizations than other LTP sensitizations in Northern Europe. The absence of a correlation between the IgE levels to Art v 3 and Par j 2, that has also been shown by others [6], points to different sensitization pathways, and is a consequence of the low structural homology between the two pollen LTPs [26,27]. …”

Section: Discussionmentioning

confidence: 64%

Mugwort-Sensitized Individuals from North Europe, South Europe and North America Show Different IgE Reactivity Patterns

Movérare¹,

Larsson²,

Carlsson³

et al. 2010

Int Arch Allergy Immunol

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Background: Sensitization to weed pollen allergens at the molecular level is not fully understood. We studied IgE reactivity to the major mugwort allergen Art v 1 in relation to allergens from other weed pollen and cross-reactive components in mugwort-sensitized subjects. Methods: Art v 1 and Amb a 1 were affinity purified and coupled to experimental ImmunoCAP® tests. Samples from North Europe (n = 50), South Europe (n = 19) and North America (n = 41) were analyzed for IgE against mugwort pollen, weed allergen components, pan-allergens and cross-reactive carbohydrate determinants (CCDs). Results: The prevalence of IgE reactivity (>0.35 kU_A/l) to Art v 1 was significantly higher in samples from North Europe than in those from North America. IgE to Amb a 1 was more common in North America than in North and South Europe, while IgE to Par j 2 was common in South Europe, less common in North America, and absent in North Europe. IgE to Art v 3 in mugwort-allergic patients was more common in North Europe than in South Europe and North America, while IgE to Sal k 1 was similar between the areas. Subjects with an Art v 1/mugwort-specific IgE ratio <0.5 had more often IgE to Amb a 1, profilin, polcalcin and CCDs than subjects with a ratio >0.5. Conclusions: Mugwort-sensitized subjects have different IgE reactivity profiles to weed allergens, reflecting their exposure to various pollens. Subjects with a low ratio between the IgE levels to Art v 1 and mugwort have a diverse IgE reactivity profile, indicating a role for cross-reactive allergens in their mugwort sensitization.

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“…Peach Pru p 3 as LTP allergen model [1], together with apple Mal d 3, mustard Sin a 3 and wheat Tri a 14 as cross-reactive food allergens [2,13,14,15,16] and Artemisia Art v 3 as cross-reactive pollen allergen [2,7,17,18] were purified (fig. 1a) to compare their IgE-binding capacity with that of isolated olive Ole e 7 and Parietaria Par j 1 (fig.…”

Section: Resultsmentioning

confidence: 99%

“…Taking into account previous data, two different types of allergenic LTP from pollens can be considered. Mugwort Art v 3 and plane tree Pla a 3 seem to be clearly implicated in plant food-pollen co-sensitization, share around 50% of amino acid sequence with peach Pru p 3 and display partial cross-reactivity with this and other food LTP allergens [1,2,17,18,19]. In contrast, olive Ole e 7 presents less than 20% of sequence identity with Pru p 3, and Parietaria Par j 1 and Par j 2 show larger molecular sizes (14.7 and 11.3 kDa, respectively) than typical 9-kDa LTP allergens, and low levels of sequence identity (27–29%) with the peach LTP allergen [1].…”

Section: Discussionmentioning

confidence: 99%

Plant Lipid Transfer Protein Allergens: No Cross-Reactivity between Those from Foods and Olive and <i>Parietaria</i> Pollen

Tordesillas¹,

Sirvent²,

Díaz‐Perales³

et al. 2011

Int Arch Allergy Immunol

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Background: Cross-reactivity among plant food allergens belonging to the nonspecific lipid transfer protein (LTP) family is well known. In contrast, the relationship among these allergens and their putative homologs from olive (Ole e 7) and Parietaria (Par j 1) pollen has not been clarified. Methods: Sera with specific IgE to LTP allergens were obtained from peach-, mustard- and olive pollen-allergic patients. Purified LTP allergens from foods (peach, apple, mustard and wheat) and pollens (olive, mugwort and Parietaria) were tested by ELISA and ELISA-inhibition assays. Results: Plant food LTP-allergic patients showed a significantly higher number of sera (89–100 vs. 33–64%) with specific IgE and mean specific IgE levels (0.30–1.56 vs. 0.21–0.34 OD units) to the 4 food LTP allergens tested than to olive Ole e 7 and Parietaria Par j 1 pollen. ELISA-inhibition assays indicated cross-inhibition between food LTP allergens but no cross-reactivity between these allergens and Ole e 7 and Par j 1, or, even more, between the LTP allergens from olive and Parietaria pollen. Conclusions: LTP allergens from olive and Parietaria pollen cross-react neither with allergenic LTPs from plant foods nor between themselves. Therefore, both pollens do not seem to be related with the LTP syndrome.

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Isoform identification and characterization of Art v 3, the lipid-transfer protein of mugwort pollen

Cited by 44 publications

References 36 publications

Lettuce Allergy Is a Lipid Transfer Syndrome-Related Food Allergy With a High Risk of Severe Reactions

Lettuce Allergy Is a Lipid Transfer Syndrome-Related Food Allergy With a High Risk of Severe Reactions

Mugwort-Sensitized Individuals from North Europe, South Europe and North America Show Different IgE Reactivity Patterns

Plant Lipid Transfer Protein Allergens: No Cross-Reactivity between Those from Foods and Olive and <i>Parietaria</i> Pollen

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