Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster

Ji, Chen; Choi, Min Sung; Mizoguchi, Akira; Veenstra, Jan A.; Kang, Kook Hee; Kim, Young-Joon; Kwon, Jae Young

doi:10.1016/j.peptides.2015.01.002

Cited by 33 publications

(34 citation statements)

References 32 publications

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“…Enteroendocrine cells secrete factors [13-15] that promote ISC proliferation [16, 17], influence cell fate choice [18, 19], regulate intestinal peristalsis and defecation [6, 20], and inhibit lipogenesis in enterocytes [21]. Interspersed among the enterocytes and ee cells, along the entire length of the midgut, are intestinal stem cells (ISCs) [22, 23].…”

Section: The Midgutmentioning

confidence: 99%

Maintenance of the adult Drosophila intestine: all roads lead to homeostasis

Guo

Lucchetta

Rafel

et al. 2016

Current Opinion in Genetics & Development

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Maintenance of tissue homeostasis is critical in tissues with high turnover such as the intestinal epithelium. The intestinal epithelium is under constant cellular assault due to its digestive functions and its function as a barrier to chemical and bacterial insults. The resulting high rate of cellular turnover necessitates highly controlled mechanisms of regeneration to maintain the integrity of the tissue over the lifetime of the organism. Transient increase in stem cell proliferation is a commonly used and elaborate mechanism to ensure fast and efficient repair of the gut. However, tissue repair is not limited to regulating ISC proliferation, as emerging evidence demonstrates that the Drosophila intestine uses multiple strategies to ensure proper tissue homeostasis that may also extend to other tissues.

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Section: The Midgutmentioning

confidence: 99%

Maintenance of the adult Drosophila intestine: all roads lead to homeostasis

Guo

Lucchetta

Rafel

et al. 2016

Current Opinion in Genetics & Development

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“…(1) Brown et al, 1999; (2) Rohwedder et al, 2015; (3) Mercer et al, 2011; (4) Schoofs et al, 1993; (5) Siviter et al, 2000; (6) Nässel et al, 1998; (7) Holzer and Holzer-Petsche, 1997; (8) Hökfelt et al, 2001; (9) Coast et al, 2001; (10) Johnson et al, 2005; (11) Brugge et al, 2008; (12) LaJeunesse et al, 2010; (13) Vanderveken and O'Donnell, 2014; (14) Sternini, 1991; (15) Kendig et al, 2014; (16) Rattan and Tamura, 1998; (17) Anselmi et al, 2005; (18) Kaminski et al, 2002; (19) Veenstra, 2009; (20) Corleto, 2010; (21) Farhan et al, 2013; (22) Veenstra and Ida, 2014; (23) Ren et al, 2015; (24) Sano et al, 2015; (25) Takizawa et al, 2005; (26) Sterkel et al, 2012; (27) Chen et al, 2015; (28) Carlsson et al, 2013; (29) Slade and Staveley, 2016; (30) Bhatt and Horodyski, 1999; (31) Duve et al, 2000; (32) Rankin et al, 2005; (33) Audsley et al, 2008; (34) Heinonen et al, 2008; (35) Duve et al, 1994; (36) Audsley and Weaver, 2009; (37) Dockray, 2009.…”

Section: Figurementioning

confidence: 99%

bHLH proneural genes as cell fate determinants of entero-endocrine cells, an evolutionarily conserved lineage sharing a common root with sensory neurons

Hartenstein

Takashima

Hartenstein

et al. 2017

Developmental Biology

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Entero-endocrine cells involved in the regulation of digestive function form a large and diverse cell population within the intestinal epithelium of all animals. Together with absorptive enterocytes and secretory gland cells, entero-endocrine cells are generated by the embryonic endoderm and, in the mature animal, from a pool of endoderm derived, self-renewing stem cells. Entero-endocrine cells share many structural/functional and developmental properties with sensory neurons, which hints at the possibility of an ancient evolutionary relationship between these two cell types. We will survey in this article recent findings that emphasize the similarities between entero-endocrine cells and sensory neurons in vertebrates and insects, for which a substantial volume of data pertaining to the entero-endocrine system has been compiled. We will then report new findings that shed light on the specification and morphogenesis of entero-endocrine cells in Drosophila. In this system, presumptive intestinal stem cells (pISCs), generated during early metamorphosis, undergo several rounds of mitosis that produce the endocrine cells and stem cells (ISCs) with which the fly is born. Clonal analysis demonstrated that individual pISCs can give rise to endocrine cells expressing different types of peptides. Immature endocrine cells start out as unpolarized cells located basally of the gut epithelium; they each extend an apical process into the epithelium which establishes a junctional complex and apical membrane specializations contacting the lumen of the gut. Finally, we show that the Drosophila homolog of ngn3, a bHLH gene that defines the entero-endocrine lineage in mammals, is expressed and required for the differentiation of this cell type in the fly gut.

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“…For example, the middle region of the midgut, which contains a group of specialized copper cells, is acidic and resembles the mammalian stomach (Dubreuil, 2004). In addition, EEs produce at least nine gut hormone peptides: Allatostatins (AstA, AstB/MIP, AstC), Tachykinin (Tk), neuropeptide F (NPF), DH31, CCHa1, CCHa2 and Orcokinin B (Chen et al, 2015;Reiher et al, 2011;Veenstra et al, 2008;Veenstra and Ida, 2014). These hormones are produced in specific regions.…”

Section: Introductionmentioning

confidence: 99%

A cell atlas of the adult Drosophila midgut

Hung

Kirchner

et al. 2018

Preprint

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Studies of the adult Drosophila midgut have provided a number of insights on cell type diversity, stem cell regeneration, tissue homeostasis and cell fate decision. Advances in single-cell RNA sequencing (scRNA-seq) provide opportunities to identify new cell types and molecular features. We used inDrop to characterize the transcriptome of midgut epithelial cells and identified 12 distinct clusters representing intestinal stem cells (ISCs), enteroblasts (EBs), enteroendocrine cells (EEs), enterocytes (ECs) from different regions, and cardia.This unbiased approach recovered 90% of the known ISCs/EBs markers, highlighting the high quality of the dataset. Gene set enrichment analysis in conjunction with electron micrographs revealed that ISCs are enriched in free ribosomes and possess mitochondria with fewer cristae. We demonstrate that a subset of EEs in the middle region of the midgut expresses the progenitor marker esg and that individual EEs are capable of expressing up to 4 different gut hormone peptides. We also show that the transcription factor klumpfuss (klu) is expressed in EBs and functions to suppress EE formation. Lastly, we provide a web-based resource for visualization of gene expression in single cells. Altogether, our study provides a comprehensive resource for addressing novel functions of genes in the midgut epithelium.

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Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster

Cited by 33 publications

References 32 publications

Maintenance of the adult Drosophila intestine: all roads lead to homeostasis

Maintenance of the adult Drosophila intestine: all roads lead to homeostasis

bHLH proneural genes as cell fate determinants of entero-endocrine cells, an evolutionarily conserved lineage sharing a common root with sensory neurons

A cell atlas of the adult Drosophila midgut

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