Isolamento e potencial enzimático de fungos associados ao intestino de larvas de Stenochironomus Kieffer (Insecta: Diptera: Chironomidae)

Belmont-Montefusco, Enide Luciana; Oliveira, Jessica Batista de; Mar, Heloise Bentes; Santa-Rosa, Pamella S.; Hamada, Neusa; Nunes-Silva, Carlos Gustavo

doi:10.34117/bjdv6n5-347

Cited by 3 publications

(4 citation statements)

References 12 publications

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“…The present results indicated common taxa in both biomes, as is the case of the genus Penicillium, which was the most frequent in this research (40%) and occurred both in the Cerrado and in the Amazon Forest. The genus Penicillium was also found in the DT of larvae of Phylloicus in the same sites of Cerrado and Amazon Forest biomes (Santos et al, 2018) as also in larvae of Triplectides, Phylloicus and Stenochironomus aquatic insects in streams sampled in another site of the Amazon Forest in the State of Amazonas, Brazil, together with Aspergillus and Trichoderma (Belmont-Montefusco et al, 2020a;Belmont-Montefusco et al, 2020b). In addition, the species Paraphaeosphaeria arecacearum, found in the DT of Triplectides in Cerrado, was also isolated in the DT of Phylloicus in the same Cerrado sites by Santos et al (2018).…”

Section: Discussionmentioning

confidence: 94%

“…Fungi have been often found in the DT of several insects that feed on wood or detritus and, possibly, play a role in the digestion of such plant material (Engel and Moran, 2013;León et al, 2016;Santos et al, 2018;Belmont-Montefusco et al, 2020a, Belmont-Montefusco et al, 2020b. Shredder insects feed on senescent plant material in low-order streams (Graça et al, 2001;Jabiol and Chauvet, 2012) where they contribute to the breakdown of organic matter in aquatic ecosystems together with fungal groups such as Ascomycetes and Hyphomycetes (Graça et al, 2016).…”

Section: Characterization Of the Study Areasmentioning

confidence: 99%

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Diversity and enzymatic capabilities of fungi associated with the digestive tract of larval stages of a shredder insect in Cerrado and Amazon Forest, Brazil

2022

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Tropical biomes such as Brazilian Cerrado and Amazon Forest have a great diversity of fungi and insects. Interactions between these organisms can be beneficial to both partners. In streams, these interactions contribute to litter decomposition. Studying the digestive tract (DT) of shredder insects as a habitat for fungal microorganisms is an opportunity to obtain fungal strains with biotechnological potential, which may help to understand the symbiotic relationships between these organisms in tropical forests. This study investigated the fungal community in the DT of larvae of Triplectides (Trichoptera: Leptoceridae) collected in low-order streams in the Cerrado and Amazon Forest biomes in Brazil. Forty-nine fungal isolates were obtained and identified among 32 species and 12 genera. The genus Roussoella was only found in the DT of insects in Amazon Forest streams, while 7 genera only occurred in the DT of insects in Cerrado streams. The genus Penicillium (40%) was the most frequent. In the Cerrado, 78% were producers of CMCase, more than two-fold that in the Amazon Forest (35%). And 62% were producers of xylanase, in the Cerrado and 71% in the Amazon Forest. In this context, the fungal community in the DT of Triplectides larvae may play an important role in the insect diet by breaking down lignocellulosic material.

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Section: Discussionmentioning

confidence: 94%

Section: Characterization Of the Study Areasmentioning

confidence: 99%

Diversity and enzymatic capabilities of fungi associated with the digestive tract of larval stages of a shredder insect in Cerrado and Amazon Forest, Brazil

2022

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“…The fungal community associated with larvae on leaves in the Cerrado was more cellulolytic (XYL=62.5% and CMC=81.2%) (Figure 3C). Corroborating such result, Belmont-Montefusco et al (2020b), who assessed the cellulolytic activity of fungi associated with the DT of larvae of Stenochironomus from leaves in Amazonian streams, found that 86.6% of the isolates were producers of that enzyme. It is supposed, therefore, that one of the adaptations of Stenochironomus larvae is related to obtaining fungi that aid in the digestion of more refractory materials such as trunks or labile such as leaves, according to their availability.…”

Section: Influence Of the Substrate On Species Distributionmentioning

confidence: 96%

Diversity of cellulolytic and xylanolytic fungi associated with the digestive tract of aquatic insect larvae in streams of the Amazon Forest and Cerrado in Brazil

2022

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The study of the relationship between fungi and insects brings important contributions to the knowledge of fungal biodiversity and to the understanding of mutualistic ecological interactions. This study reports the occurrence of a community of filamentous fungi in the digestive tract (DT) of mining insect larvae belonging to genus Stenochironomus in streams of two Brazilian biomes. Fungi were obtained from the digestive tract of larvae found on trunks and leaves of low-order streams in the Amazon Forest and Cerrado in the north of Brazil. The fungal community was screened for xylanolytic and cellulolytic activities. The diversity of fungal species in the DT of larvae is possibly related to the diversity of diets of species of that genus and the diversity of substrates in the ecosystems. The diversity and richness of fungal species were influenced by ecological differences between locations more than by the types of substrates in which they were collected (trunk and leaf). Most fungi in the DT of Stenochironomus larvae sampled in leaves exhibited cellulolytic enzyme activity. Such results stress that the mycobiomes of the DT of Stenochiromonus larvae produce enzymes that contribute to the process of breaking down plant remains in their hosts.

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“…Segundo Reis (2017), os besouros da ambrosia (Coleoptera: Curculionidae: subfamílias Platypodinae e Scolytinae), também conhecidos como brocas, vivem em simbiose com diferentes microrganismos (fungos filamentosos, leveduras, bactérias e protozoários) que habitam o sistema digestivo dos mesmos. Montefusco (2020) e Milano (2012) atestam em suas pesquisas a presença de espécies fúngicas com potencial enzimático que estão associados ao intestino de larvas e pragas próprias da cana-de-açúcar. Uma avaliação da flora do trato digestório de besouros pode ser uma fonte grande de descobrimento de novos compostos e espécies fúngicas.…”

Section: Introductionunclassified

Bioprospecção de fungos isolados do intestino de besouros amazônicos da ordem Coleoptera com atividade anti-candida sp.

Rufino

Rodrigues²,

Abegg³

2023

RSD

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Com uma atividade exploratória desenfreada dos recursos naturais, muito da biodiversidade mundial vem diminuindo com o passar dos anos. Com toda a riqueza Amazônica dá-se a vantagem ao Brasil pela busca pelo desenvolvimento nacional, através da exploração adequada destes recursos, minimizando danos ao ambiente. Neste sentido a Bioprospecção surge como uma ferramenta do Desenvolvimento Sustentável, que pode ser entendida como a busca sistemática por organismos, genes, enzimas, compostos, processos e partes provenientes de seres vivos, que tenham potencial econômico e eventualmente, levam ao desenvolvimento de um produto. Muitos fungos vivem em associações simbiônticas ou comensais em intestinos de Besouros e estes fungos possuem uma capacidade ainda desconhecida de produção de substâncias ainda desconhecidas. Tendo em vista estas relações, esta pesquisa objetiva realizar uma Bioprospecção de fungos filamentosos extraídos do intestino de besouros da Amazônia. Os resultados são promissores ao destacar a atividade antifúngica contra cepas de espécies de Cândida sp com as frações de extratos estudadas proveniente de espécie de Fusarium Incarnatum-equisetti.

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Isolamento e potencial enzimático de fungos associados ao intestino de larvas de Stenochironomus Kieffer (Insecta: Diptera: Chironomidae)

Cited by 3 publications

References 12 publications

Diversity and enzymatic capabilities of fungi associated with the digestive tract of larval stages of a shredder insect in Cerrado and Amazon Forest, Brazil

Diversity and enzymatic capabilities of fungi associated with the digestive tract of larval stages of a shredder insect in Cerrado and Amazon Forest, Brazil

Diversity of cellulolytic and xylanolytic fungi associated with the digestive tract of aquatic insect larvae in streams of the Amazon Forest and Cerrado in Brazil

Bioprospecção de fungos isolados do intestino de besouros amazônicos da ordem Coleoptera com atividade anti-candida sp.

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