ISOLASI DAN IDENTIFIKASI BAKTERI Aeromonas sp. DARI LELE DUMBO (Clarias sp.) DI KABUPATEN NGAWI

Rejeki, Sri; Triyanto, Triyanto; Murwantoko, Murwantoko

doi:10.22146/jfs.26917

Cited by 10 publications

(10 citation statements)

References 1 publication

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“…Indonesian researchers have previously identified Aeromonas species from catfish by phenotypic identification and collected three Aeromonas species, namely, A. hydrophila , A . caviae , and A. salmonicida [ 35 , 60 , 61 ]. Six species, including A. hydrophila , A. salmonicida , A .…”

Section: Discussionmentioning

confidence: 99%

Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)

Mulia,

Pratiwi,

Asmara

et al. 2023

Vet World

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Background and Aim: The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas spp. isolated from diseased catfish. Materials and Methods: Nine Aeromonas spp. were isolated from infected catfish cultivated in Java, Indonesia, and they were identified at the phenotypic and molecular levels (16S rDNA). The virulence genes assessed included aer/haem, alt, ast, flaA, lafA, and fstA. Results: Phylogenetic analysis identified nine isolates of Aeromonas spp.: Aeromonas hydrophila (11.11%), Aeromonas caviae (11.11%), Aeromonas veronii bv. veronii (44.44%), and Aeromonas dhakensis (33.33%). Virulence genes, such as aer/haem, alt, ast, flaA, lafA, and fstA, were detected in all isolates at frequencies of approximately 100%, 66.67%, 88.89%, 100%, 55.56%, and 66.67%, respectively. This study is the first report on A. dhakensis recovered from an Indonesian catfish culture. Furthermore, our study revealed the presence of A. veronii bv veronii, a biovar that has not been reported before in Indonesia. Conclusion: This finding confirms that MAS was caused by multiple species of Aeromonas, notably A. dhakensis and A. veronii bv veronii, within Indonesian fish culture. The presence of these Aeromonas species with multiple virulence genes poses a significant threat to the freshwater aquaculture industry. Keywords: 16S rDNA, aeromoniasis, motile Aeromonas septicemia, pathogenicity, phenotype, phylogenetic.

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Section: Discussionmentioning

confidence: 99%

Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)

Mulia,

Pratiwi,

Asmara

et al. 2023

Vet World

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“…Hasil pemeriksaan sampel (Tabel 1) menunjukkan bahwa seluruh sampel yang diperiksa tidak terinfeksi bakteri A. salmonicida dan E. ictalury. (Rejeki et al, 2016). Serangan penyakit ini digolongkan mejadi dua yaitu golongan sub-akut atau kronis dan golongan akut.…”

Section: Hasil Dan Pembahasanunclassified

Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region

Rahmawati¹,

Ulkhaq²,

Susanti³

et al. 2021

JMCS

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The freshwater aquaculture fishery sector in Indonesia has the potential to be developed through extensification and intensification. The problem in aquaculture is diseases that infects cultivated organisms, one of which is caused by bacterial infection. Bacterial infection can cause huge losses to cultivation activities, including death which results in economic losses. The purpose of this study is to identify the A. salmonicida and E. ictalury in live fish that will be exported from DI Yogyakarta. The method of examining bacteria used is conventional which consists of isolation, purification, presumptive test, biochemical test, and identification. The results showed that all live fish samples (koi, gouramy, goldfish, catfish, and eel) were not infected with A. salmonicida and E. ictalury. This shows that all live fish are in a healthy condition and suitable to be exported of DI Yogyakarta.

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“…Berbagai penelitian isolasi dan identifikasi penyakit bakterial telah dilakukan diantaranya berbagai jenis Aeromonas, Vibrio, Pesudomonas dari ikan gurami (Murwantoko et al, 2013), bakteri Aeromonas dari ikan lele (Clarias sp.) (Rejeki et al, 2016). Tetapi sangat terbatas penelitian penyakit pada E. tarda di Indonesia.…”

Section: E D W a R D S I E L L A T A R D A T E R M A S U K D A L A M unclassified

Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta

Murwantoko

Diniarti

Triyanto

2019

JFS

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Edwarsiella tarda is a cosmopolitan bacterium and is a cause of Edwardsiellosis in various fish species. The bacterial infection causes large losses on aquaculture in Asia, especially Japan. This study was conducted to isolate and characterize E. tarda as causative disease in freshwater fishes, and to determine its pathogenicity to catfish (Pangasius sp.). Bacteria were isolated from kidney of diseased fishes on Tryptone Soya Agar medium. Identification was conducted based on morphological colonies, morphological cells and biochemical tests. Fulfillment of Koch Postulates was done by injecting bacteria intraperitoneally on 7-9 cm fishes at dose of 107 cfu/fish. Pathogenicity test was carried out by intraperitoneal injection at 104, 105, 106, and 107 cfu/fish to 7-9 cm-catfish (Pangasius sp.) and followed by observation of disease signs and mortality every six hours for 7 days. Pathogenicity was determined as Lethal Dosage (LD50) using Dragstedt Behrens method. In this research we have isolated three isolates E. tarda causing disease in fishes. The clinical signs of this disease were lose of pigmentation over the lession, swollen of stomach, haemorhage on fins , small cutaneous lesions, and necrotic on fins area. The LD50 of E. tarda isolate L2, L3, and N3 were 4.64 ± 0.35x105, 1.54 ± 0.07x105, and 1.13 ± 0.13x106 cfu/fish, respectively.

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ISOLASI DAN IDENTIFIKASI BAKTERI Aeromonas sp. DARI LELE DUMBO (Clarias sp.) DI KABUPATEN NGAWI

Cited by 10 publications

References 1 publication

Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)

Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)

Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region

Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta

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