Isolasi dan identifikasi bakteri toleran logam berat dari sedimen mangrove di Pengudang dan Tanjung Uban, Pulau Bintan, Indonesia

Abstract

<strong>Isolation and Identification of Heavy Metals Tolerant Bacteria from Mangrove Sediment in Pengudang and Tanjung Uban, Bintan Island, Indonesia</strong>. Bacterial searches for remediation of heavy metal pollutants continue to be carried out due to the increasing pollution in the ecosystem as a result of industrialization. This research aims to identification bacteria strains that are tolerant of heavy metals from mangrove sediments in the Pengudang and Tanjung Uban, Bintan Island in 2018. Bacterial isolation was carried out by pour plate method and purified using marine agar (MA) medium. Screening of bacterial isolates was carried out by growing isolates on MA medium with the addition of 100 ppm Pb. In addition, the determination of minimum inhibitory concentration (MIC) was performed on the isolates were tolerant to Pb, Cu, and Cd by addition of heavy metals in 50-1000 ppm concentrations. Identification of heavy metal tolerant isolates was carried out by sequencing the 16S rRNA gene. The results of bacterial isolation from mangrove sediments obtained 48 isolates used for testing the ability of heavy metals tolerant. Screening tests showed that PGD1A, PGD5A, PGD22A, PGD1B, PGD9B, PGD5C, PGD6C, TJU1, TJU5, and TJU7 isolates were heavy metal resistant. The results showed that bacterial isolated from mangrove sediments had MIC values of 800-900 ppm for Pb, 100-800 ppm for Cu and 100-200 ppm for Cd. There are 3 isolates that have high resistance to three kinds of heavy metals such as Bacillus oceanisediminis PGD1A, Vibrio alginolyticus PGD5A, and Halobacillus kuroshimensis PGD9B. This indicates that the isolate can be used efficiently for removal of heavy metals from the ecosystem.

Untitled

“…In this study, we observed that Vibrio dominated mangrove and beach samples. Nevertheless, the genus Vibrio is predominantly endemic to marine aquatic environments, ranging from the deep sea to shallow aquatic environments (Junaid et al, 2022); tolerant of heavy metals isolated from mangrove sediments (De Fretes et al, 2019), and sewage-associated plastic wastes (Metcalf et al, 2022).…”

CONCENTRATION OF HEAVY METALS IN GREEN MUSSELS (Perna viridis) OF LAMPUNG BAY AND THEIR SYMBIONE BACTERIAL RESISTANCE

“…(5'-TACGGHTACCTTGTTACGACTT-3') (De Fretes et al, 2019). Amplification used a temperature profile of 1 min at 95˚C followed by 30 cycles of denaturation (95˚C, 1 min), annealing (55˚C, 45 s) and extension (72˚C, 1 min), with final extension at 72˚ C for 10 minutes.…”

“…In addition, there are bacteria that can be tolerate Pb heavy metal levels up to a concentration of 2000 ppm (Imamuddin, 2001) The results of the Cu resistance test showed that bacterial isolates with both isolate codes of STL11 and STL25 could survive up to a concentration of 400 ppm toward Cu and that of isolates code STL09 had the highest level of resistance, up to 700 ppm against Cu. Previous research stated that bacteria have resistance to heavy metals up to 800 ppm copper (Cu) (De Fretes et al, 2019).…”

Isolasi Dan Karakterisasi Bakteri Resisten Cd Dari Kawasan Penambangan Timah

“…Penelitian terdahulu telah ditemukan beberapa isolat bakteri resisten Cd dari berbagai sumber dan dengan kemampuan resistensi yang berbeda-beda. Fretes et al, (2019) berhasil mengisolasi 3 bakteri resisten Cd dengan konsentrasi 100-200 ppm, serta oleh Priyalaxmi et al, (2014) yang mendapatkan isolat bakteri resisten Cd pada konsentrasi 60 ppm dan 80 ppm. Nath et al, (2012) Sampel sedimen dikeringkan menggunakan oven dengan suhu 105°C selama 1x24 jam.…”