Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

Wandita, Ryan Hilda; Pujiyanto, Sri; Suprihadi, Agung; Hastuti, Ratih Dewi

doi:10.14710/bioma.20.1.9-16

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…NaCN formed through cyanide gas absorption by NaOH or Na2CO3 through the reaction between sodium and ammonia that initially will be formed NaNH2 that will react with the carbon and will produce sodium cyanamide (Na2NCN) and eventually will be formed NaCN which is one type of cyanide. This shows that the brighter the color, the higher the isolates caused more HCN content of sodium cyanide were joined by ammonia (4). The color differences can be seen in Figure 1.…”

Section: Results and Discussion 1 Ability Test Gas Hydrogen Cyanide (Hcn)mentioning

confidence: 87%

The Growth Responses of Potato Crops (Solanum tuberosum L.) in various type of Rhizobacteria and Mycorrhiza

Putri¹,

Warnita²,

Satria³

2020

IJEAB

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Potato (Solanum tuberosum L.) is a plant prefetch queue of five seasonal vegetables that are much in demand in Indonesia. Potato needs tend to increase every year in line with population growth and development of the food processing industry are made from raw potatoes. In general, potato production in Indonesia is still relatively low and still do to meet their import requirements. But the government has always sought to boost the national potato production. One of the efforts to improve potato production with the use of Plant Growth Promoting Rhizobacteria (PGPR) and fungi Mycorrhizal Fungi (AMF). The use of microorganisms PGPR and Mycorrhizae naturally associated with plant roots and have the ability to improve plant growth. The purpose of this study is to obtain the best interaction between species rhizobacteria and mycorrhiza on the growth of potato plants. This study is a two-factor factorial experiment with three replications in a randomized block design. First factor is type rhizobacteria which are without rhizobacteria, RZ3.L2.1, RZ3.L2.2, RZ3.L2.5. The second factor is the type of mycorrhizal wich are Glamus mycorrhiza, mycorrhizal and mycorrhizal Acaulospora sclerocystis, Data were analyzed by analysis of variance and DNMRT 5%. The results showed that the best treatment for the growth of the potato crop is rhizobakteria RZ3.L2.2 with mycorrhizal glamus. Keywords-potatoes, rhizobacteria, arbuscular mycorrhizal fungi. II. MATERIALS AND METHODS Penelitian di Nagari Baruah Gunuang Kecamatan BukitResearch in Nagari District of Bukit Barisan Gunuang Baruah, District Fifty Cities with altitude ± 1000 masl. The experiment was conducted from November 2019 -Februari in 2020 and preparation of bacterial isolates Mikrobiology indigenous conducted at the Laboratory, Faculty of Agriculture, University of Andalas Padang, Materials used are seed potatoes, isolates Rhizobakteri, FMA, water, paper labels, plastic samples, envelopes, manure, lime, fertilizers NPK, The experiment was arranged according to the design of 4 x 3 with two factors in a randomized block design (RBD) with three replications. The treatments were

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Section: Results and Discussion 1 Ability Test Gas Hydrogen Cyanide (Hcn)mentioning

confidence: 87%

The Growth Responses of Potato Crops (Solanum tuberosum L.) in various type of Rhizobacteria and Mycorrhiza

Putri¹,

Warnita²,

Satria³

2020

IJEAB

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“…Kemampuan bakteri endofit dalam menekan pertumbuhan cendawan patogen tanaman dikarenakan kemampuan bakteri dalam menghasilkan senyawa volatil salah satunya berupa HCN (Hidrogen Sianida). Wandita et al (2018) Pengujian senyawa volatil yang dilakukan pada 10 isolat bakteri endofit menunjukkan potensi menghambat pertumbuhan F. oxysporum secara in-vitro dengan tingkat hambat relatif senyawa volatil yang dihasilkan berbeda-beda tiap isolat, diperoleh 2 isolat bakteri endofit dengan persentase tingkat hambat relatif diatas 60%, yaitu isolat ASR-7(3) dan BK-7(2), Adanya perbedaan tingkat hambat senyawa volatil tersebut diduga karena adanya perbedaan kemampuan isolat bakteri endofit dalam menghasilkan senyawa volatil.…”

Section: Tingkat Hambat Relatif Uji Volatil Terhadap Fusarium Oxysporumunclassified

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

RAHIMI

KHAERUNI²,

BANDE³

et al. 2022

Agroteknos

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This research motivated by the Fusarium oxysporum which is a soil borne fungus that can infect cultivated plants. F. oxysporum is a facultative parasite that is part of its life as a saprophyte and becomes a parasite if there is a host plant. This study aims to determine the potential of endophytic bacteria from wild plants as biological agents control of F. oxysporum in-vitro. This research was conducted at the Laboratory of Plant Protection, Phytophatology Unit, Faculty of Agriculture, Halu Oleo University from September 2020 to March 2021. The results showed that endophytic bacteria isolated from wild plants such as as Jamaica cherry/kersen (Muntingia calabura), Suruhan (Paperomia pellucida), and Markisa Hutan/Rambusa (Passiflora foetida) have the potential as biological agents to control F. oxysporum in vitro, with the two best isolates, namely ASR-7(3) and BK-3(2) which were to inhibit the growth of F. oxysporum through the mechanism of antibiosis and competition, produced enzyme bioactive compounds (amylase and protease) which degrade of pathogenic fungi, and are able to produce volatile compounds.

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“…dan diperoleh 40 isolat, 27 di antaranya dapat melarutkan fosfat (Matos et al, 2017). Tanaman bawang merah (Allium cepa L.) yang diisolasi dan diperoleh 230 isolat bakteri endofit, 31 di antaranya dapat melarutkan fosfat (Wandita et al, 2018). Namun, sejauh ini belum ada penelitian terkait bakteri endofit yang dapat melarutkan fosfat dari tumbuhan akar kuning asal Pulau Enggano.…”

Section: Pendahuluanunclassified

Kemampuan Bakteri Endofit Pelarut Fosfat dari Tumbuhan Akar Kuning (Arcangelisia flava (L.) Merr) Asal Pulau Enggano, Provinsi Bengkulu

Wibowo

Sembiring

Sipriyadi

et al. 2022

Al-Kauniyah J. Biol.

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AbstrakFosfor merupakan salah satu unsur makro nutrien yang dibutuhkan untuk perkembangan tumbuhan dan dapat dihasilkan oleh bakteri pelarut fosfat. Namun, keberadaan fosfat dalam tumbuhan tidak dapat digunakan oleh tumbuhan itu sendiri, sehingga dibutuhkan bantuan bakteri endofit yang berperan sebagai agen pemacu pertumbuhan tanaman yang dapat melarutkan fosfat pada tumbuhan, khususnya tumbuhan akar kuning (Arcangelisia flava (L.) Merr) yang tumbuh liar di Pulau Enggano, Provinsi Bengkulu. Tujuan penelitian ini untuk memperoleh bakteri endofit yang dapat melarutkan fosfat. Isolasi dilakukan dengan metode sterilisasi permukaan, menggunakan alkohol 70% dan natrium hipoklorit 5,25% lalu ditempelkan di media NA. Isolat yang diperoleh kemudian diuji kemampuan melarutkan fosfat dengan digoreskan ke media Pikovskaya, lalu diukur zona bening. Hasil penelitian menunjukkan dari 18 isolat bakteri endofit, 8 di antaranya dapat melarutkan fosfat. Isolat AKE15 dan AKE18 memiliki kemampuan lebih tinggi untuk melarutkan fosfat dengan indeks pelarut fosfat (IP) ± 1,59 dan ± 1,01. Identifikasi berdasarkan pewarnaan Gram dan uji biokimia dari 8 isolat bakteri endofit pelarut fosfat (BPF) memiliki kedekatan dengan 4 genus, yaitu Bacillus, Micrococcus, Pseudomonas, dan Morococcus.AbstrakPhosphorus is one of the macronutrients required for plant development and produced by phosphate solubilizing bacteria. However, plants are unable to utilize phosphate themselves, thus they need the help of endophytic bacteria that play role as plant growth-promoting with ability to solubilize phosphates in plants, particularly in yellow root plants (Arcangelisia flava (L.) Merr) that grow wildly in Enggano Island, Bengkulu Province. The research aimed to obtain endophytic bacteria that can solubilize phosphate. Isolation of endophytic bacteria was carried out by surface sterilization method using 70% alcohol and 5.25% sodium hypochlorite to be further placed on Nutrient Agar (NA) media. The isolates obtained were further tested for their ability to solubilize phosphate by streaking the isolate on Pikovskaya media and measuring the clear zones formed. The results showed that 8 out of 18 endophytic bacterial isolates were able to solubilize phosphate. Endophytic isolates of AKE15 and AKE18 were found to have higher ability to solubilize phosphate with a phosphate solubilizing index (IP) of about ± 1.59 and ± 1.01, respectively. Identification based on Gram staining and biochemical tests indicated that 8 endophytic bacterial solubilizing isolates were closely related to 4 genera, namely Bacillus, Micrococcus, Pseudomonas, and Morococcus.

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Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

Cited by 5 publications

References 7 publications

The Growth Responses of Potato Crops (Solanum tuberosum L.) in various type of Rhizobacteria and Mycorrhiza

The Growth Responses of Potato Crops (Solanum tuberosum L.) in various type of Rhizobacteria and Mycorrhiza

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

Kemampuan Bakteri Endofit Pelarut Fosfat dari Tumbuhan Akar Kuning (Arcangelisia flava (L.) Merr) Asal Pulau Enggano, Provinsi Bengkulu

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