Isolasi dan Karakterisasi Bakteri Selulolitik pada Tanah Gambut di Desa Tagagiri Tama Jaya, Kecamatan Pelangiran, Kabupaten Inhil, Riau

Fauziah, Siti Isnaini; Ibrahim, Muslimin

doi:10.26740/lenterabio.v9n3.p194-203

Cited by 3 publications

(7 citation statements)

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“…Uji gelatin digunakan untuk mengetahui kemampuan bakteri dalam menghidrolisis gelatin menjadi asam amino dengan menghasilkan enzim gelatinase (Fauziah & Ibrahim, 2020).…”

Section: Moraxellaunclassified

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Isolasi dan Aktivitas Bakteri Selulolitik pada Limbah Diapers

Siruwahni,

Rasyidah

2023

BIOEDUSAINS

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This study aims to isolate cellulolytic bacteria that have the potential to degrade cellulose by taking home-scale diaper waste samples. This research was also conducted to isolate and determine the ability of bacteria to degrade cellulose based on the Congo red test and filter paper degradation test. This research method is descriptive qualitative. Isolation of cellulolytic bacteria was carried out using selective Carboxymethyl Cellulose (CMC) media. The characterization was carried out by growing selected pure isolates on CMC media and then dropping congored drops to test their cellulolytic potential (cellulolytic potential is indicated by the appearance of a clear zone around the colony). The results showed that the results of isolating bacteria obtained 12 isolates that were able to grow and utilize cellulose as a carbon source. In the Congo red test isolate DS07 cellulolytic bacteria of the Hafnia genus had the highest cellulolytic index value of 2.5 mm. Isolate DS03 with the genus Bacillus had the highest ability to degrade cellulose on the Whatman No. filter paper test. 1 of 16.66%. Keywords: Bacteria, Diapers, Isolation, Cellulolytic

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“…Uji gelatin digunakan untuk mengetahui kemampuan bakteri dalam menghidrolisis gelatin menjadi asam amino dengan menghasilkan enzim gelatinase (Fauziah & Ibrahim, 2020).…”

Section: Moraxellaunclassified

“…Hal ini menujukkkan hanya terjadi reaksi fermentasi glukosa saja. Begitu pula pada isolat yang berwarna kuning pada keseluruhan media menunjukkan reaksi asam dan terjadi fermentasi glukosa, sukrosa, dan laktosa (Fauziah & Ibrahim, 2020).…”

Section: Moraxellaunclassified

Isolasi dan Aktivitas Bakteri Selulolitik pada Limbah Diapers

Siruwahni,

Rasyidah

2023

BIOEDUSAINS

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“…Bakteri memainkan peran penting dalam ekosistem mangrove terutama dalam mengurai serasah daun dan kayu lapuk (Yahya et al, 2018). Bakteri selulolitik pada serasah dan kayu diindikasikan memiliki kemampuan mendegradasi serat dan selulosa sehingga mempercepat pelapukan disekitar wilayah mangrove (Kurniawan et al, 2018) Bakteri selulolitik memiliki kemampuan dalam mendegradasi komponen kompleks selulosa ke bentuk yang lebih sederhana yaitu glukosa (Fauziah & Ibrahim, 2020). Menurut Rudiansyah et al (2017), bakteri selulolitik memiliki tingkat pertumbuhan yang lebih cepat dibandingkan bakteri jenis lainnya sehingga produksi bakteri selulolitik hanya membutuhkan waktu yang singkat.…”

Section: Pendahuluanunclassified

“…Menurut Rudiansyah et al (2017), bakteri selulolitik memiliki tingkat pertumbuhan yang lebih cepat dibandingkan bakteri jenis lainnya sehingga produksi bakteri selulolitik hanya membutuhkan waktu yang singkat. Fauziah & Ibrahim (2020) menyebutkan bahwa genusgenus bakteri yang umumnya dapat mendegradasi selulosa antara lain adalah anggota dari genus Nisseria, Micrococcus, Bacillus, Pseudomonas, flavobacterium, dan Actinobacillus.…”

Section: Pendahuluanunclassified

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Isolasi dan Karakterisasi Bakteri Selulolitik Dari Lumpur Mangrove Pantai Pandaratan Kecamatan Sarudik Kabupaten Tapanuli Tengah Provinsi Sumatera Utara

Ananda,

Rasyidah,

Mayasari

2023

Bioma

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Cellulolytic bacteria are bacteria that have the ability to degrade cellolose into glucose by utilizing celullase enzymes. The purpose of this study was to determine the presence of cellulolytic bacteria and to characterize isolates from mangrove mud of Pandaratan Beach, Sarudik District, Central Tapanuli Regency, North Sumatera Province. Cellulolytic bacteria were isolated from mangrove mud using CMC media with the pour plate method. Bacterial characteristics were seen based on morphological, gram staining and physiology observations. The Cellulolytic activity test was based on the cellulolytic index (IS) after staining with 0,1% congo red. The isolataion results obtained 14 isolates of cellulolytic bacteria. A total of 7 isolates were cellulolytic bacteria and were characterized based on category level. High category SL4, medium category SL3; SL5; SL8; and low category SL7; SL9; SL11. Based on the results of morphological and phylogical characterization 3 members of the cellulolytic bacterial genus were found, namely Cellulomonas (SL3 and SL8), Bacillus (SL4; SL5; and SL11), and Micrococcus (SL7 and SL9). The conclusion of this study was found as many as 7 isolates of cellulolytic bacteria from the mangrove mud of Pandaratan Beach, Sarudik District, Central Tapanuli Regency, North Sumatera Province, namely the genera Cellulomonas, Bacillus, and Micrococcus.

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Isolasi dan Uji Aktivitas Selulolitik Bakteri Asal Limbah Bagas

Chusniasih

Suryanti

Safitri

2023

J. Ilmu. Pertan. Indones.

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Bagasse is one of the by-products of granulated sugar industries or processed beverages that become waste in the environment. The bagasse will be overgrown by cellulolytic bacteria that produce cellulase enzymes. This study aims to isolate, characterize, and measure bacterial cellulase activity qualitatively and quantitatively. The research phase included isolation and characterization of bacteria, qualitative cellulase activity test, preparing standard curves and bacterial growth curves, crude extract cellulase production, constructing standard glucose curves, quantitative enzyme activity tests, and data analysis. Six isolates with macroscopic characteristics varied in color, elevation, margins, and colony shape; three bacillus isolates, one short bacillus isolate, and two cocci isolates were obtained. All isolates showed positive test results of amylum, triple sugar iron agar, and catalase; five citrate-positive isolates, and three motile-positive isolates. From the cellulase qualitative test, two bacterial isolates had the highest cellulolytic index, namely AT1 (1.79) and BAW3 (1.72) on 0.5% CMC media. The quantitative cellulase test results of the two selected isolates were 0.01176 U/mL for AT1 and 0.01170 U/mL for BAW3, both of which are still classified as low degradation capabilities. Keywords: cellulase activity, cellulolytic index, Sugarcane waste

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Isolasi dan Karakterisasi Bakteri Selulolitik pada Tanah Gambut di Desa Tagagiri Tama Jaya, Kecamatan Pelangiran, Kabupaten Inhil, Riau

Cited by 3 publications

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Isolasi dan Aktivitas Bakteri Selulolitik pada Limbah Diapers

Isolasi dan Aktivitas Bakteri Selulolitik pada Limbah Diapers

Isolasi dan Karakterisasi Bakteri Selulolitik Dari Lumpur Mangrove Pantai Pandaratan Kecamatan Sarudik Kabupaten Tapanuli Tengah Provinsi Sumatera Utara

Isolasi dan Uji Aktivitas Selulolitik Bakteri Asal Limbah Bagas

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