Isolasi Dan Skrining Bakteri Selulolitik Dari Beberapa Tanah Hutan Di Bali

Yusnia, Ella Dewi; Gunam, Ida Bagus Wayan; Antara, Nyoman Semadi

doi:10.24843/jrma.2019.v07.i01.p02

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Gram-positive bacteria are characterized as having a purple color, which indicates that these bacteria can bind crystal violet. In gram-positive bacteria, the cell walls contain less fat, so they become dry due to alcohol treatment, the pore size and permeability of the cell walls are reduced and crystal violet is not washed away, which causes positive bacteria to remain purple (Yusnia et al 2019). Selected Cellulolytic from PCo which has high activity were follow gram staining test and the result were presented in figure 3.…”

Section: The Effect Of Litter Quality and Soil Cellulolytic Bacterial...mentioning

confidence: 99%

The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria

ARFARITA,

NURAINI,

RINADY

et al. 2024

Biodiversitas

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Abstract. Arfarita N, Nuraini Y, Rinady MVP, Noerhayati E, Prayogo C. 2024. The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria. Biodiversitas 25: 924-936. Agroforestry leads to the accumulation of organic matter, and the environmental conditions, litter quality, and decomposing organisms influence the rate at which litter decomposes. Litter decomposition in agroforestry systems is slow due to the high lignin and phenolic substances content, low light intensity, and high humidity under the canopy's shading condition. Therefore, a study was carried out to address this issue to isolate cellulolytic bacteria capable of breaking down plant litter using a qualitative cellulase activity testing method. The complete randomized block design and Tukey's test were used to determine the treatment's significance. Six agroforestry systems were examined: pine-coffee, pine-banana, pine-cardamom, pine-cardamom, mixed garden, and citrus. The PK (Pine-Coffee) plot had the highest canopy cover, litter density, and cellulolytic bacteria. The study identified three cellulolytic bacteria isolates (PK1, PK13, and PK10) from the 50 isolated bacteria of PK plot, producing the largest clear zones on CMC media. These identified isolates belonging to the Bacillus genus were Gram-positive bacteria with rod-shaped cells. The different types of litter in agroforestry systems affect the content of lignin, polyphenols, cellulose, and C/N ratios, which can influence the abundance of cellulolytic bacteria and their potential cellulase activity.

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Section: The Effect Of Litter Quality and Soil Cellulolytic Bacterial...mentioning

confidence: 99%

The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria

ARFARITA,

NURAINI,

RINADY

et al. 2024

Biodiversitas

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“…Isolat yang tumbuh pada media CMC dimurnikan dan dilanjutkan pada diuji kemampuan degradasi selulosa. Kemampuan degradasi selulosa diuji secara kuantitatif dengan menuangkan merah kongo 0,1%, kemudian didiamkan selama 15 menit dan dibilas dengan NaCl 1 M. Isolat yang bernilai positif mampu mendegradasi selulosa ditunjukkan dengan terbentuknya zona bening di sekeliling koloni bakteri (Yusnia et al 2019). Adapun fungi lignolitik diisolasi pada medium padat selektif PDA (39 g L -1 ) yang diperkaya dengan 0,05% guaiakol.…”

Section: Isolasi Bakteri Selulolitik Dan Fungi Lignolitikunclassified

Pengaruh Aplikasi Bioarang dan Zeolit pada Fluks CO2, Populasi Mikrob, dan Aktivitas Enzim Mikrob pada Gambut

Aprianti¹,

Widyastuti²,

Pulunggono³

et al. 2023

J. Ilmu. Pertan. Indones.

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Oil palm plantation on peatlands is considered to contribute to increasing global warming by releasing CO2 as one of the greenhouse gases that cause global warming. The addition of ameliorants, such as biochar and zeolite, can absorb CO2 from soil respiration and store and filter it in their molecular pores. This research aimed to study the effect of ameliorant application on the CO2 flux, water content, microbial population, and enzyme activities. Ameliorant was applied to peat soil with two factors. The first factor was an ameliorant combination with 6 treatment levels: control (A0); zeolite (A1); biochar (A2); zeolite:biochar 75:25; zeolite:biochar 25:75; zeolite:biochar 50:50, and the second factor was 3 levels dose of treatment (% w/w): 1.5%, 3%, and 4.5%. Peat flux CO2 and water content were measured monthly for four months in the laboratory. Without mixing with biochar (A1), the results showed that zeolite suppressed CO2 flux and suppressed the decrease of water content better than other ameliorant combinations. However, different ameliorant dosage percentages did not show any significant results. Ameliorant application and incubation affected the microbial population and enzyme activities, and the results were varied. Chemical characteristics changed after 4 months of incubation. In conclusion, our results indicate that only zeolite amendment can suppress CO2 flux and decrease water content but did not affect microbial population dynamics and enzyme activities. Keywords: ameliorant, CO2 flux, enzyme activities, lignocellulolytic microbes, peat

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“…It was done using four pieces of OPEFB soaked in detergent for one minute and rinsed with distilled water. Removal of water content was carried out at a temperature of 105 °C for five hours (Yusnia et al, 2019).…”

Section: Determination Of Dry Weight Percentage Of Opefbmentioning

confidence: 99%

Isolation of Cellulose-Degrading Bacteria from Luwu Timur in Oil Palm Empty Fruits Bunch

Febryanti¹,

Baharuddin²,

Rahayu³

2021

WJC

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The oil palm empty fruit bunch (OPEFB) is one of the solid wastes produced by industrial factories. These bunches are the habitat of cellulolytic bacteria. This study aims to identify the morphology and determine the potential microorganisms in degrading oil palm empty fruit bunches waste. The methods used in this study were isolation and observation of bacterial morphology, bacterial screening, degradation test of empty oil palm fruit bunches waste, and enzyme activity test with DNS method using UV-Vis spectrophotometer at a wavelength of 530 nm. A collection of 14 isolates were obtained from the isolation process. We obtained 14 isolates from the sample, the largest clear zone was shown by isolate S10. The isolate also exhibited the highest cellulose-degrading activity after 7 days incubation period with a degrading percentage of 13.27%. After incubation for 2 days, the morphological characteristics of the bacteria produced have circular and medium-sized colonies, yellow, colonies wave edges and flat colonies, elevation convex and smooth surface. The resulting enzyme activity was 0.1308 U/mL. Therefore, isolate S10 was suspected to have the potential to degrade plastic.

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Isolasi Dan Skrining Bakteri Selulolitik Dari Beberapa Tanah Hutan Di Bali

Cited by 4 publications

References 6 publications

The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria

The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria

Pengaruh Aplikasi Bioarang dan Zeolit pada Fluks CO2, Populasi Mikrob, dan Aktivitas Enzim Mikrob pada Gambut

Isolation of Cellulose-Degrading Bacteria from Luwu Timur in Oil Palm Empty Fruits Bunch

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