ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO

Abstract

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.

Review Vibriosis Management in Indonesian Marine Fish Farming

“…Outbreak of vibriosis have been reported in seven species of marine fish farming in Indonesia ( Table 1). The susceptible fish are snapper [15] and grouper [5]. Outbreak of vibriosis was also reported in tiger shrimp Penaeus monodon [7,16], abalone [17], dan pacific white shrimp Lithopenaeus vannamei [14].…”

“…Most Vibrio species will grow on that medium within 24 hours of incubation to form green or yellow colonies. Vibrio parahaemolyticus, V. alginolyticus, V. furnisii, V. vulnificus and V. anguillarum are likely to form greenish yellow colonies, while V. methcnikovii and V. fluvialis formed yellow colonies [5]. For routine maintenance, Vibrio bacteria grow well on marine agar, zobell, 50% seawater complex agar medium [20], TSA trisalt [19] and Nutrient Agar [10] nd: no data…”

“…It is the main bacterial disease of cultured marine fish and shrimps in Indonesia [4]. The disease was most predominantly reported in grouper [5,6]. A summary of the species found as causative agents of vibriosis in Indonesian marine culture is shown in table 1.…”

“…In the earlier report, V. carchariae was known to correlated with vibriosis in shrimp [7] and V. anguillarum, V. alginolyticus, V. parahaemolyticus, and V. marinus were associated with vibriosis in grouper [8]. Since then, between 2000-2010, more species were found to contribute to vibriosis in fish and shrimps, including V. alginolyticus, V. parahaemolyticus, V. anguillarum, V. vulnificus, V. metchnikovii, V. Furnisii, V. Fluvialis, V. ordalii, and V. cincinnatiensis [5,9,10]. Thereafter, Vibriosis in Indonesian fish farming were associated with the infection of V. harveyii, V. alginolyticus, V. vulnificus, V. anguilarum, V. parahaemolyticus, V. azureus, V. damsela (Photobacterium damsela subspecies piscicida), V. mimicus and V. fluvialis [11,12,13,6,14,15].…”

Sensitivitas Antibakteria Dari Tanaman Caulerpa sp. Dan Enteromorpha sp. Terhadap Bakteri Vibrio alginolyticus

“…Ditambahkan NaCl 2,5 % sebanyak 600 ml, dimasukkan ke microwave sampai homogen. Hasil penelitian ini serupa dengan hasil penelitian sebelumnya yaitu bakteri V.alginolyticus termasuk bakteri Gram negatif, bentuk koloni menyebar, oksidase dan katalase positif, bersifat fermentatif, motil dan indol positif (Nitimulyo, et. al., 2005;Luturmas & Pattinasarany, 2010;Johnny & Roza, 2014;Dahlia, et.…”