Isolate-specific neutralizing antibodies in patients with progressive HIV-1-related disease

Gegerfelt, Agneta von; Albert, Jan; Morfeldt-Månson, Linda; Broliden, Kristina; Fenyõ, Éva Mária

doi:10.1016/0042-6822(91)90764-3

Cited by 68 publications

(40 citation statements)

References 23 publications

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“…It has been suggested that the evolution of HIV during the course of infection in a single individual is the result of selection by antibody for escape variants (28). Because there have been complete Env sequences obtained from L.W.F.…”

Section: Resultsmentioning

confidence: 99%

Temporal analysis of the antibody response to HIV envelope protein in HIV-infected laboratory workers.

Pincus¹,

Messer²,

Nara³

et al. 1994

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 99%

Temporal analysis of the antibody response to HIV envelope protein in HIV-infected laboratory workers.

Pincus¹,

Messer²,

Nara³

et al. 1994

J. Clin. Invest.

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“…Most PBMC neutralization assays monitor virus growth by assaying for extracellular expression of viral proteins such as p24 antigen (p24-Ag) or reverse transcriptase (1,3,41,46,75). These assays require several rounds of virus replication before expressed proteins can be quantified and thus only indirectly measure the actual number of target cells infected.…”

Section: Hiv-1-infected Pbmc Serial Dilutions Of Virus Stocks Demonsmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Neutralization Measured by Flow Cytometric Quantitation of Single-Round Infection of Primary Human T Cells

Mascola

Louder

Winter

et al. 2002

J Virol

106

101

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“…During progression of the disease, increasing amounts of cell-free virus (CFV) are detectable in plasma [14,15] and genetic variation promotes escape from recognition both by neutralizing antibodies and cytotoxic T lymphocytes (CTL) [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Escape of HIV-1 is associated with lack of V3 domain-specific antibodies in vivo

Schreiber

Müller

Wachsmuth

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThis study was performed to analyse correlates of viral escape in AIDS patients. Peripheral blood mononuclear cells (PBMC) from HIV ¹ donors were inoculated with AIDS patients' serum to detect neutralization-resistant cell-free virus. Infectious virus was detected by polymerase chain reaction (PCR) and analysed by sequencing the V3 region. The escaped virus species was compared with all V3 virus variants found in the patients' PBMC and plasma. In one patient escaped virus was also compared with variants found in CD4 þ T cells isolated by FACS from blood, spleen and lymph node. The frequency of the virus variants was determined by cloning and sequence analysis of 20 V3 clones for each PCR amplification. To monitor anti-V3 antibodies by ELISA, each V3 sequence was expressed as fusion with glutathione S-transferase (GST-V3). In our AIDS patients, a V3-directed antibody response against the infectious virus V3 loop was not detectable. In contrast, virus variants unable to infect the donor PBMC in vitro were well recognized by homologous V3-directed antibody. After an interval of 1 year the frequency of these variants clearly decreased, while at the same time the escaped variants grew out and finally represented the predominant viral species both in plasma and PBMC. The infectious variants lacking V3 antibody response were also predominant in CD4 þ T cells in spleen and lymph node. Our data indicate that the escape of virus variants is closely related to the lack of V3-directed antibody.

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Isolate-specific neutralizing antibodies in patients with progressive HIV-1-related disease

Cited by 68 publications

References 23 publications

Temporal analysis of the antibody response to HIV envelope protein in HIV-infected laboratory workers.

Temporal analysis of the antibody response to HIV envelope protein in HIV-infected laboratory workers.

Human Immunodeficiency Virus Type 1 Neutralization Measured by Flow Cytometric Quantitation of Single-Round Infection of Primary Human T Cells

Escape of HIV-1 is associated with lack of V3 domain-specific antibodies in vivo

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