Isolated Adrenal Cells: Corticosterone Production in Response to Cyclic AMP (adenosine-3',5'-monophosphate)

Sayers, George; Ma, Rose-Marie; Giordano, Nicholas D.

doi:10.3181/00379727-136-35325

Cited by 15 publications

(7 citation statements)

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“…They report that isolated adrenal cells respond to ACTH with an increased steroidogenesis; when combined with Quso® (mi crofi ne silica granules) treatment, plasma ACTH levels in peripheral blood could be adequately determined. In further confirmation of the method, it was found that the isolated adrenal cells responded not only to ACTH but to cyclic AMP as well (127) .…”

Section: Control Of the Secretion Of Adrenocorticotropinmentioning

confidence: 95%

Hypothalamic Control of Adenohypophysial Secretions

Blackwell¹,

Guillemin²

1973

Annu. Rev. Physiol.

152

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This chapter has no pretense at being an exhaustive review of all the lit erature published over the last few years pertinent to its title. To the contrary, we want to make clear that we have selected about one-fifth of what we col lected in preparation of this review, to be included here. The basis for selection of the literature quoted here is simply explained in terms of our own interests or shortcomings. If what we say in this chapter or how we say it generates some argument, we feel that there will be none for the statement that the most important contributions over the last few years to the efforts at under standing the mechanisms involved in the physiological control of adenohypo physial functions by the hypothalamus reside in the characterization of two of the hypothalamic hypophysiotropic hormones, TRF and LRF. The recent characterization and total synthesis by this laboratory of a hypothalamic peptide that specifically inhibits the secretion of immunoreactive growth hor mone, may have similar significance and implications as it would represent the first of the long-postulated hypothalamic hypophysiotropic inhibitory factors for adenohypophysial secretions.We think it of interest that this review should discuss a large number of clinical reports of physiological significance, thus showing the wide impact in clinical medicine and clinical investigation of the elucidation of the structure of TRF and LRF and their availability in unlimited quantities by total synthe sis. Once more, basic research will have contributed to the practice of medicine and the welfare of innumerable patients. ABBREVIATIONSWe use the following abbreviations in the text as defined here:releasing factor 357 1100 Annu. Rev. Physiol. 1973.35:357-390. Downloaded from www.annualreviews.org Access provided by University of California -Davis on 02/06/15. For personal use only. Quick links to online content Further ANNUAL REVIEWS

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Section: Control Of the Secretion Of Adrenocorticotropinmentioning

confidence: 95%

Hypothalamic Control of Adenohypophysial Secretions

Blackwell¹,

Guillemin²

1973

Annu. Rev. Physiol.

152

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“…It has been shown that ACTH receptor interactions are rapid, requiring only milliseconds (7), with the subsequent formation o f B after a 3-to 5-min lag period (15). If ACTH and CAPE were competing for the same receptor site, then the inhibitory effect o f CAPE should be manifest immediately.…”

Section: Discussionmentioning

confidence: 99%

“…The interaction o f ACTH and its receptor to produce B lias been shown to be analogous to that o f a substrate and its enzyme to produce a product (15). The use o f this analogy implied a number o f assumptions: (1) the calculated doses o f ACTH are equivalent to the amount available to interact with the receptor; (2) one molecule o f ACTH interacts with one receptor to form a hormone-receptor complex, and (3) the measured production of B is directly related to the amount of ACTH receptor complex so formed.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effects of a Pineal Extract on Adrenal Cortex Lack of Competition with ACTH

Heiman¹,

Porter²

1980

Horm Res

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The purpose of the present investigation was to study the mode of action of a crude aqueous pineal extract (CAPE) on corticosterone (B) production from ACTH-mediated isolated adrenal cortex cells. Corticosterone production from a heterogenous adrenal cortex cell population, isolated from 8 male Sprague-Dawley rats, was measured fluorimetrically. CAPE (25 µl) was tested in this system using ACTH (0–5,000 pg/ml) and dibutyryl-c-AMP (0–100 nA//ml) as stimuli for a period of 1 h. In a separate experiment, CAPE (25 µl) was administered to ACTH (50 pg/ml) stimulated adrenal cortex cells for 15, 30, 60, and 120 min incubation periods. CAPE significantly decreased B produced by adrenal cortex cells at all doses of ACTH administered. CAPE also decreased the B produced by adrenal cortex cells when dibutyryl-c-AMP was used as a stimulus. The inhibitory effect of CAPE was manifest at some point in time between 30 and 60 min. It was significant at 60 min and highly significant at 120 min. It is evident from these data that CAPE and ACTH are not competing for the same receptor site.

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“…The steroidogenic response of isolated adrenal-cell suspensions can provide a sensitive, accurate bioassay for corticotrophins (Sayers et al, 1971;Lowry et al, 1973). However, to be able to interpret potency differences obtained from any bioassay of peptide-hormone analogues it is necessary to understand their fate in the assay system.…”

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confidence: 99%

Fate of corticotrophins in an isolated adrenal-cell bioassay and decrease of peptide breakdown by cell purification

Bennett¹,

Bullock²,

Lowry³

et al. 1974

Biochemical Journal

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1. The fate of corticotrophins in a trypsin-dispersed rat adrenal-cell assay system was investigated with a view to establishing whether differences in the rate of inactivation might contribute to potency differences observed between analogues. 2. Corticotrophin-(1-24)-tetracosapeptide and to a lesser extent synthetic 1-39 corticotrophins were found to be inactivated during incubation with cell suspension. 3. Peptide fragments were isolated by using [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide as a marker. The fragments indicate a peptidase with a predominantly tryptic specificity. 4. The peptidase is present in the extracellular fluid and is released from cells when they are damaged. 5. Cells were fractionated on an albumin gradient. Cells from the zona fasciculata and the zona intermedia or reticularis were present in fractions which produced fluorogenic steroids in response to corticotrophin. 6. Purification of the cells by centrifugation through albumin decreased degradation by peptidases, so that if the assay is carried out with a dilute suspension of purified cells peptide breakdown should not affect the observed potencies of adrenocorticotrophin analogues. 7. No binding of [[(3)H(2)]Tyr(23)]corticotrophin-(1-24)- tetracosapeptide to cells could be detected at low concentrations of the peptide. This indicated that less than 120 receptors/cell are occupied during stimulation by a dose that would elicit approx. 80% of the maximal response.

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Isolated Adrenal Cells: Corticosterone Production in Response to Cyclic AMP (adenosine-3',5'-monophosphate)

Cited by 15 publications

References 0 publications

Hypothalamic Control of Adenohypophysial Secretions

Hypothalamic Control of Adenohypophysial Secretions

Inhibitory Effects of a Pineal Extract on Adrenal Cortex Lack of Competition with ACTH

Fate of corticotrophins in an isolated adrenal-cell bioassay and decrease of peptide breakdown by cell purification

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