Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR

Abstract

Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. Based on 16S rDNA and BIOLOG test analysis, the 330-2 strain was identified as Bacillus subtilis. The strain produced indole-3-acetic acid, siderophores, lytic enzymes and solubilized different sources of organic/inorganic phosphates and zinc. Furthermore, the strain strongly suppressed the in vitro growth of Rhizoctonia solani AG1-IA, Botrytis cinerea, Fusarium oxysporum, Alternaria alternata, Cochliobolus heterostrophus, and Nigrospora oryzae. The strain also significantly increased the seedling growth (ranging 14–37%) of rice and maize. Removing PCR analysis indicated that 114 genes were differentially expressed, among which 10%, 32% and 10% were involved in antibiotic production (e.g., srfAA, bae, fen, mln, and dfnI), metabolism (e.g., gltA, pabA, and ggt) and transportation of nutrients (e.g., fhu, glpT, and gltT), respectively. In summary, these results clearly indicate the effectiveness and mechanisms of B. subtilis strain 330-2 in enhancing plant growth, as well as tolerance to biotic/abiotic stresses, which suggests that the strain has great potential for commercialization as a vital biological control agent.

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

“…So, microbial management of FS by utilizing endophytic Bacillus strains may offer a potential and viable solution to replace health hazardous and environmentpolluting chemicals. Endophytic B. amyloliquefaciens, B. subtilis, and B. cereus strains with antifungal activity towards diverse types of crop pathogens have been well documented [64][65][66][67]. The present investigation revealed that endophytic B. amyloliquefaciens (EPP35, EPP 42, EPP62, and EPP 102), B. subtilis subsp.…”

Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections

“…Fusarium oxysporum f. sp. conglutinans ) ( Ahmad et al, 2017 ; Duijff et al, 1993 ; Sherriff and Lucas, 1990 ; Slusarenko and Schlaich, 2003 ; Sulochana et al, 2014 ). Applications of the H30-3 to the Chinese cabbage for disease management of downy mildew and Fusarium wilt remains investigated.…”

Molecular and Physiological Logics of the Pyruvate-Induced Response of a Novel Transporter in Bacillus subtilis

“…Root exudates are composed of carboxylic acids, such as malate, citrate, and pyruvate, and vary across environments, specifically in response to the presence of phytotoxic compounds ( 2 ). Root exudates and released carboxylic acids also enable recruiting beneficial bacteria, such as the Gram-positive model bacterium Bacillus subtilis , that help to reduce susceptibility to plant pathogen attack ( 3 – 5 ). The assimilation of carboxylic acids by B. subtilis mainly relies on active transport systems, whose expression is induced via two-component systems (e.g., maeN by MalK/R [ 6 ]) by the transported carbon source ( 7 ).…”