Isolated hepatocytes of rainbow trout (Oncorhynchus mykiss) as a tool to discriminate between differently contaminated small river systems

Strmac, Marija; Braunbeck, Thomas

doi:10.1016/s0887-2333(00)00031-x

Cited by 37 publications

(22 citation statements)

References 54 publications

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“…The present study was designed to serve four purposes: (1) to investigate the potential cytotoxicity of a complex toxicant mixture to isolated hepatocytes from rainbow trout; (2) to identify, localize, and compare biochemical and ultrastructural alterations in hepatocytes induced by the mixture of chemicals; (3) to compare biochemical and ultrastructural alterations in hepatocytes with cytological e!ects induced by native waters and sediment extracts of the river KoK rsch (Strmac and Braunbeck, 2000); (4) to compare cytological e!ects with those observed in similar investigations.…”

Section: Discussionmentioning

confidence: 99%

“…Di!erent studies have investigated the action of chemical mixtures in vivo on rats (Constan et al, 1995;Simmons et al, 1994), mice (Hong et al, 1992), rabbits, goats, ducks (Williams et al, 1993), and "sh (KoK nemann, 1981;Nagel, 1995, 1997) and in vitro using human neuronal cells (Marinovich et al, 1996), human lymphocytes (Dolara et al, 1994), isolated mouse hepatocytes (Klauning et al, 1990), fetal quail hepatocytes (Hugla et al, 1996), and rat brain cells (Segal, 1989). A series of studies with isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) exposed to pure compounds (Braunbeck, 1993;Braunbeck, 1993, 1995;Zahn et al, 1996), chemical mixtures of unknown composition , and acetone river sediment extracts (Strmac and Braunbeck, 2000) demonstrated that, under standardized test conditions, toxicity tests with cell cultures represent a practicable tool for the assessment of lethal and sublethal toxicity in aquatic systems (Braunbeck, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytological and Biochemical Effects of a Mixture of 20 Pollutants on Isolated Rainbow Trout (Oncorhynchus mykiss) Hepatocytes

Strmac

Braunbeck

2002

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytological and Biochemical Effects of a Mixture of 20 Pollutants on Isolated Rainbow Trout (Oncorhynchus mykiss) Hepatocytes

Strmac

Braunbeck

2002

Ecotoxicology and Environmental Safety

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“…Thus, the replacement or reduction of in vivo fish tests by in vitro cytotoxicity tests (Braunbeck and Strmac 2001, Segner 1998, Strmac and Braunbeck 2000 as well as embryo assays (Ensenbach 1998, Strmac et al 2002 have repeatedly been recommended. Given the high correlation with data from the fish test, both the cytotoxicity test and the fish embryo assay may provide good estimates of the potential toxicity of sediment-bound contaminants.…”

Section: Comparison Of Embryo Toxicity and Acute Toxicity In The Permmentioning

confidence: 99%

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Hollert

Keiter

König

et al. 2003

J Soils & Sediments

201

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos. Soils and Sediments, http://dx.doi.org/10.1065/jss2003.09.085 Journal ofAccess to the published version may require subscription. Conclusions.A modified protocol of the zebrafish embryo test allows the assessment of sediment toxicity in both aqueous extracts and native sediments. The isolated investigation of pore waters may result in a clear-cut underestimation of the bioavailability of lipophilic particle-bound substances (as determined by native sediments). Recommendations and Perspectives.The zebrafish embryo test with native (whole, solid phase) sediments appears very promising for the evaluation of the bioavailable fraction of lipophilic particle-bound substances and can therefore be recommended for the evaluation of vertebrate toxicity in tiered sediment test strategies and dredging directives such as the HABAB-WSV. Whereas acetone extracts may be tested as a rough estimation of embryotoxicity, native sediment samples will provide a more comprehensive and realistic insight into the bioavailable hazard potential. (2) to compare the hazard potential of solid phase sediments to the effects of corresponding pore waters and organic extracts in order to characterize the bioavailability of the particle-bound pollutants; and (3) to relatively evaluate the embryotoxic effects of sediments from the catchment areas of the rivers Rhine, Neckar and Danube. Keywords Methods (or Main Features).To investigate the toxicity of sediment samples on vertebrates, the standard embryo toxicity test with the zebrafish (Danio rerio; Hamilton-Buchanan 1922) according to DIN 38415-6 was modified with respect to exposure scheme and toxicological endpoints. Sediments from the catchment area of the Neckar River were assessed using pore waters, acetonic extracts and native sediments in order to get inside into the potential bioavailability of particle-bound pollutants. A comprehensive test protocol for the investigation of native sediments in the embryo toxicity test with the zebrafish is presented.Results and Discussion. The fish embryo assay with Danio rerio can be carried out with both aqueous and organic sediment extracts as well as native (whole, solid phase) sediment samples. Elongation of exposure time from 48 to up to 196 h significantly increased the mortality. Using the fish egg assay with native sediments, a broad range of embryotoxic effects could be elucidated, including clear-cut dose-response curves for the embryotoxic effects of contaminated sediments; in contrast, absence of embryotoxic effects could be demonstrated even for the highest test concentrations of unpolluted sediments. With native sediments, embryotoxicity was clearly higher than with corresponding pore waters, thus corroborating the view that -at least for fish eggs -the bioavailability of particle-bound lipophilic substances in native sediments is higher than generally assumed. The relative ranking of sediment toxicity was identical using both nati...

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“…Strmac and Braunbeck (Strmac and Braunbeck, 2000) exposed isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) to native river waters and sediment extracts from two small river systems. Significant differences in terms of toxic burden between the two rivers as well as between free water phase and sediment were detected by applying different end points focusing on cytological and biochemical changes.…”

Section: Reviewmentioning

confidence: 99%

“…The study by Kinani 2010) showed that the hepatoma fish cell line PLHC-1 is a suitable model system to detect dixoin-like compounds in sediments, which was also demonstrated in a study by Schnell et al (Schnell et al, 2013) where controls sites could be clearly teased apart from contaminated sites. Strmac and Braunbeck (Strmac and Braunbeck, 2000) exposed isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) to native river waters and sediment extracts from two small river systems. Significant differences in terms of toxic burden between the two rivers as well as between free water phase and sediment were detected by applying different end points focusing on cytological and biochemical changes.…”

Section: Sediment Extractsmentioning

confidence: 99%

Gill cell culture systems as models for aquatic environmental monitoring

Bury

Schnell

Högstrand

2014

Journal of Experimental Biology

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A vast number of chemicals require environmental safety assessments for market authorisation. To ensure acceptable water quality, effluents and natural waters are monitored for their potential harmful effects. Tests for market authorisation and environmental monitoring usually involve the use of large numbers of organisms and, for ethical, cost and logistic reasons, there is a drive to develop alternative methods that can predict toxicity to fish without the need to expose any animals. There is therefore a great interest in the potential to use cultured fish cells in chemical toxicity testing. This review summarises the advances made in the area and focuses in particular on a system of cultured fish gill cells grown into an epithelium that permits direct treatment with water samples. KEY WORDS: FIGCS, Biomonitoring, Environmental risk assessment, Fish, In vitro, Toxicology IntroductionThe Industrial Revolution caused a rapid rise in the use of raw materials and urbanisation as the populace moved to the cities for employment. Since this time, there has been a continuous increase in living standards that to a large part has been fuelled by innovations within the chemical and pharmaceutical industry. Life expectancy has increased as a result of great advances in medical practices and effective drugs against many fatal diseases. The increase in life expectancy has seen the population of the world grow, reaching 7 billion in 2012, and to feed this population there have been great advances in agricultural productivity partly via the development of pesticides and nitrate/phosphate-based fertilisers. These activities have altered the geochemical cycling of elements, increasing or decreasing concentrations in earth system compartments and increasing global distribution (Doney, 2010). Anthropogenic activities have left a cumulative and lasting impression on the biosphere -so much so that geologists have termed the current epoch the anthropocene (Zalasiewicz et al., 2010).The increase in agricultural and industrial production and consumption of raw materials has resulted in the creation of vast amounts of waste that enters the aquatic ecosystem. An acknowledgement of the decline in environmental quality due to contaminants has led to the development of environmental quality standards (EQS) in many countries, and to assess whether these standards are being adhered to, many jurisdictions also have a programme of waste water effluent testing (WET) and/or biomonitoring. The EQS are derived from toxicity tests that use numerous organisms per compound, and in order to set standards several species are tested. In the USA ~3 million fish are used in WET procedures (see Tanneberger et al., 2013). There is a move towards reducing the number of animals used in research and toxicology studies and there are a number of international initiatives aimed at investigating the 3Rs -reduction, replacement and refinement -in animal research (for example, see http://www.nc3rs.org.uk/). Within the context of the need to determine EQS fo...

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Isolated hepatocytes of rainbow trout (Oncorhynchus mykiss) as a tool to discriminate between differently contaminated small river systems

Cited by 37 publications

References 54 publications

Cytological and Biochemical Effects of a Mixture of 20 Pollutants on Isolated Rainbow Trout (Oncorhynchus mykiss) Hepatocytes

Cytological and Biochemical Effects of a Mixture of 20 Pollutants on Isolated Rainbow Trout (Oncorhynchus mykiss) Hepatocytes

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Gill cell culture systems as models for aquatic environmental monitoring

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