Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media I. Effects of pretreatment, isolation methods, and hormones

Hu, Tianci; Ziauddin, A.; Simion, E.; Kasha, K. J.

doi:10.1007/bf02632241

Cited by 46 publications

(18 citation statements)

References 20 publications

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“…Hu et al (1995) report that appropriate androgenesis-inducing pretreatment of Anemona canadensis reduced endogenous ABA concentrations at the end of pretreatment by nearly four times, whereas Imamura and Harada (1980) demonstrate a peak in endogenous ABA levels after 24 h of mannitol pretreatment in tobacco anthers. Our results, demonstrating that for both cultivars there was a peak of ABA in anthers after the first 24 h of pretreatment with mannitol (Fig.…”

Section: Discussionmentioning

confidence: 99%

The Role of Abscisic Acid in Induction of Androgenesis: A Comparative Study Between Hordeum vulgare L. Cvs. Igri and Digger

Bergen¹,

Kottenhagen²,

Meulen³

et al. 1999

J Plant Growth Regul

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Under the same mannitol pretreatment and culture conditions, regeneration efficiency in the barley cultivar (cv.) Igri was about 10 times higher than in the cv. Digger, a difference only partially reflected by a difference in viable microspores after anther pretreatment. Therefore, a comparative study between cvs. Igri and Digger was carried out under various pretreatment conditions. For both cultivars, under water, CPW buffer and mannitol pretreatment conditions, there was a positive correlation between microspore viability and regeneration efficiency in that mannitol > CPW buffer >> water. Mannitol pretreatment of cv. Igri produced a much higher endogenous abscisic acid (ABA) level than as to Digger. Addition of ABA stimulated both percentages of viability and regeneration efficiency except in the case of mannitol pretreatment. Under CPW buffer pretreatment conditions, addition of ABA significantly stimulated regeneration efficiency and was ABA concentration dependent. However, cv. Digger was less responsive to ABA than cv. Igri. In both cultivars, under less optimal pretreatment conditions (e.g., water and CPW buffer), the effect of ABA was to stimulate increased percentages of viability and/or to reduce the number of binucleate microspores. Moreover, in cv. Igri, direct culture of anthers for 4 days without pretreatment caused an increased number of binucleate microspores compared with microspores with pretreatment for 4 days. These binucleate microspores showed DNA degradation in the nuclei. However, with mannitol pretreatment binucleate microspores and DNA fragmentation in the nuclei of microspores was rarely observed. On the basis of our observations, we suggest that the difference in regeneration efficiency in cv. Igri and cv. Digger is related to the differences in endogenous ABA production levels under mannitol pretreatment and responsiveness to ABA. One of the effects of ABA is likely due to an inhibition of cell death.

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Section: Discussionmentioning

confidence: 99%

The Role of Abscisic Acid in Induction of Androgenesis: A Comparative Study Between Hordeum vulgare L. Cvs. Igri and Digger

Bergen¹,

Kottenhagen²,

Meulen³

et al. 1999

J Plant Growth Regul

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“…Therefore, the genetic stability of microspore-derived plants should be higher. The recent improvement of embryogenesis and green plant regeneration from isolated microspore culture of wheat has increased the potential use of isolated microspore culture (Mejza et al 1993;Hu et al 1995). Therefore, the investigation of genetic stability and performance of microsporederived doubled haploid plants is warranted.…”

Section: Mots Clésmentioning

confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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. 1997. Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.). Can. J. Plant Sci. 77: 549-554. Ploidy level, genetic stability and field performance of isolated microspore-derived wheat plants were evaluated. The ploidy levels of isolated microspore-derived wheat plants from cv. Chris and reciprocal crosses of Chris × Sinton were determined by two methods, namely chromosome counts of root tip cells and flow cytometric measurement of nuclear DNA content from leaves. Both methods gave similar results with the frequencies of spontaneous chromosome doubling and completely fertile plants among microspore-derived H 0 plants of wheat being about 80% and 75%, respectively, based on two H 0 populations. Only 1.7% were aneuploid and 16% were haploid. Spontaneous mutation frequencies were low with 1 of 124 Chris DH plants having a recessive mutant for lemma awns while three were grass-like plants in the H 0 generation. The field-measured traits of microspore-derived DH lines were similar to the check by the third generation. Ninety-two percent and 70% of Chris DH lines had 1000-kernel weights and yields similar to the check, respectively. These results indicate that microspore-derived DH lines should have good potential for applications in plant breeding, in vitro selection, plant transformation and genetic studies.

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“…However, according to some researches, in an optimal plant growth regulator combination, many dividing microspores evolve into true embryoids that have very high germination rates. Additionally, the inclusion of plant growth regulator (PGR) such as abscisic acid (ABA) (Hu et al 1995), 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) (Liu et al 2002) in the pretreatment phase is also beneficial for embryogenic induction.…”

Section: Effects Of Other Factors On Anther Culturementioning

confidence: 99%

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide

Bal¹,

Shariatpanahi²,

Castro³

et al. 2012

Czech J. Genet. Plant Breed.

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This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudoembryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos' commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation.

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Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media I. Effects of pretreatment, isolation methods, and hormones

Cited by 46 publications

References 20 publications

The Role of Abscisic Acid in Induction of Androgenesis: A Comparative Study Between Hordeum vulgare L. Cvs. Igri and Digger

The Role of Abscisic Acid in Induction of Androgenesis: A Comparative Study Between Hordeum vulgare L. Cvs. Igri and Digger

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide

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