Isolated Myeloperoxidase Immunohistochemical Expression in Bone Marrow Biopsy Depicts Clinical Outcomes in Adults with Typical B-Acute Lymphoblastic Leukemia

Fawzy, Mohammed E.; El-Hafez, Amal Abd; El-Ashwah, Shaimaa; Abouzeid, Tarek; Waheeb, Maryan Fahmi; Saif, Maha; Mortada, Metwaly Ibrahim; Ghannam, Mayada A.; Sheta, Heba

doi:10.31557/apjcp.2021.22.7.2143

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Notably, myeloperoxidase (MPO), as a common marker to distinguish between the myeloid and lymphoid lineages, was mainly expressed in the low oxidative stress score group according to our result. Previous contradictory study has addressed its clinical significance in pediatric B-acute lymphoblastic leukemia (B-ALL) patients [ 37 ], and Kuang et al [ 38 ] identified MPO as a risk gene in AML patients. This contradictory result may be due to the discrepancy between individuals and the biological functions of MPO involved in different pathways.…”

Section: Discussionmentioning

confidence: 99%

Combined scRNAseq and Bulk RNAseq Analysis to Reveal the Dual Roles of Oxidative Stress-Related Genes in Acute Myeloid Leukemia

Lin

et al. 2023

Oxidative Medicine and Cellular Longevity

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Background. Oxidative stress (OS) can either lead to leukemogenesis or induce tumor cell death by inflammation and immune response accompanying the process of OS through chemotherapy. However, previous studies mainly focus on the level of OS state and the salient factors leading to tumorigenesis and progression of acute myeloid leukemia (AML), and nothing has been done to distinguish the OS-related genes with different functions. Method. First, we downloaded single-cell RNA sequencing (scRNAseq) and bulk RNA sequencing (RNAseq) data from public databases and evaluated the oxidative stress functions between leukemia cells and normal cells by the ssGSEA algorithm. Then, we used machine learning methods to screen out OS gene set A related to the occurrence and prognosis of AML and OS gene set B related to treatment in leukemia stem cells (LSCs) like population (HSC-like). Furthermore, we screened out the hub genes in the above two gene sets and used them to identify molecular subclasses and construct a model for predicting therapy response. Results. Leukemia cells have different OS functions compared to normal cells and significant OS functional changes before and after chemotherapy. Two different clusters in gene set A were identified, which showed different biological properties and clinical relevance. The sensitive model for predicting therapy response based on gene set B demonstrated predictive accuracy by ROC and internal validation. Conclusion. We combined scRNAseq and bulk RNAseq data to construct two different transcriptomic profiles to reveal the different roles of OS-related genes involved in AML oncogenesis and chemotherapy resistance, which might provide important insights into the mechanism of OS-related genes in the pathogenesis and drug resistance of AML.

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Section: Discussionmentioning

confidence: 99%

Combined scRNAseq and Bulk RNAseq Analysis to Reveal the Dual Roles of Oxidative Stress-Related Genes in Acute Myeloid Leukemia

Lin

et al. 2023

Oxidative Medicine and Cellular Longevity

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“…Although it is negative in FC, positivity in IHC has been detected in various studies, and these rates go up to 26.7%. [38] Aberrant expression of CD117 in BCP-ALL is seen as a myeloid marker. Its expression in the literature varies between 0.5-36%.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Correlation of Immunohistochemical Findings with Flow Cytometric Findings in Newly Diagnosed Pediatric Acute Lymphoblastic Leukemia Patients

Özdemir

Kar

Eroğlu

et al. 2023

Journal of Contemporary Medicine

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Background and objectives: The development of new therapeutic options to treat leukemia (therapies targeting chimeric antigen receptor [CAR] T cells) down-regulates markers expressed on the cell surface. Therefore, conventional immunophenotyping panels no longer make these antigens unreliable for identifying a B cell immunophenotype. In our study, we methodically compared multiparametric flow cytometry (FC) in bone marrow aspiration and immunohistochemical (IHC) analysis in bone marrow biopsy in childhood acute lymphoblastic leukemia (ALL). We sought to answer whether these two methods could be alternatives to each other in the diagnosis of leukemia. Material-Method: Twenty-eight patients diagnosed with ALL were included in the study. A Kappa test was performed between the expression rates of the antibodies studied in simultaneous FC and IHC studies in bone marrow aspiration and biopsy samples performed at the initial diagnosis. Results: Twenty-three of the patients were precursor B-ALL (BCP-ALL) and 5 were T-ALL. In the immunophenotyping of patients with BCP-ALL using FC and IHC, MPO, CD79A, CD14, CD3 expressions were the same, while CD19, CD7, CD117, CD33, CD 56, CD34 expressions were very good, good concordance for CD20 expressions and moderate for CD10 expressions. In immunophenotyping of patients diagnosed with T-ALL using FC and IHC, CD20, CD19, CD14, CD79a, MPO, CD22 expressions were the same and excellent agreement was found in terms of CD2, CD10, CD34 expressions. Conclusion: In cases where there are treatments that affect immunophenotyping, costly methods such as FC are not available, or bone marrow aspiration cannot be taken adequately, immunophenotyping with IHC can be safely performed in the diagnosis of pediatric ALL in bone marrow biopsy.

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Isolated Myeloperoxidase Immunohistochemical Expression in Bone Marrow Biopsy Depicts Clinical Outcomes in Adults with Typical B-Acute Lymphoblastic Leukemia

Cited by 2 publications

References 25 publications

Combined scRNAseq and Bulk RNAseq Analysis to Reveal the Dual Roles of Oxidative Stress-Related Genes in Acute Myeloid Leukemia

Combined scRNAseq and Bulk RNAseq Analysis to Reveal the Dual Roles of Oxidative Stress-Related Genes in Acute Myeloid Leukemia

Evaluation of the Correlation of Immunohistochemical Findings with Flow Cytometric Findings in Newly Diagnosed Pediatric Acute Lymphoblastic Leukemia Patients

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