Isolating and identifying fungi to determine whether their biological properties have the potential to control the population density of mosquitoes

Wasinpiyamongkol, Ladawan; Kanchanaphum, Panan

doi:10.1016/j.heliyon.2019.e02331

Cited by 14 publications

(14 citation statements)

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“…The diversity of the endophytic fungal community associated with Opuntia sp was studied and investigated for antifungal potential against phytopathogenic fungal species by Silva-Hughes and others [ 39 ]. The previous studies have shown Aspergillus tamarii reported as better larvicidal properties against mosquitoes [ 40 ]. The present study aimed to isolate, molecular identification of endophytic fungus Aspergillus tamarii from Opuntia ficus-indica and evaluate its mycelial metabolites to tested against Ae.…”

Section: Introductionmentioning

confidence: 99%

Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Baskar

Ragavendran

Pandy

et al. 2020

Heliyon

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Background Mosquitoes biolarvicides remain the most important method for mosquito control. The previous studies have shown Aspergillus sp. -expressed larvicidal properties against mosquito species. The present study evaluated larvicidal and histopathological effect of an endophytic fungus Aspergillus tamarii isolated from theCactus stem ( Opuntia ficus-indica Mill) . Method The molecular identification of isolated A. tamarii was done by PCR amplification (5.8s rDNA) using a universal primer (ITS-1 and ITS-2). The secondary metabolites of A. tamarii was tested for larvicidal activity against Aedes aegypti and Culex quinquefasciatus . Larvicidal bioassay of different concentrations (- 100, 300, 500, 800 and 1000 μg/mL) isolated extracts were done according to the modified protocol. Each test included a set of control groups (i.e. DMSO and distilled water). The lethal concentrations (LC 50 and LC 90 ) were calculated by probit analysis. Experimental monitoring duration was 48 h. Results The ethyl acetate extract from A. tamarii fungus resulted - excellent mosquitocidal effect against Ae. aegypti and Cx. quinquefasciatus mosquitoes, with least LC 50 and LC 90 values. -After 48 h, the Ae. aegypti expressed better results (LC 50 = 29.10, 18.69, 16.76, 36.78 μg/mL and the LC 90 = 45.59, 27.66, 27.50, 54.00 μg/mL) followed by Cx. quinquefaciatus (LC 50 = 3.23, 24.99, 11.24, 10.95 μg/mL and the LC 90 = 8.37, 8.29, 21.36, 20.28 μg/mL). The biochemical level of A. tamarii mycelium extract on both larvae was measured and the results shown a dose dependent activity on the level of AchE, α- and β-carboxylesterase assay. Gas Chromatography and Mass Spectroscopy (GC-MS) profile of A. tamarii extract reflected three compounds i.e. preg-4-en-3-one, 17. α-hydroxy-17. β-cyano- (7.39%), trans-3-undecene-1,5-diyne (45.77%) and pentane, 1,1,1,5-tetrachloro- (32.16%) which which might had attributed to larvae mortality. Conclusion The findings of - present study shows that the use of endophytic A. tamarii fungal metabolites for control of dengue and filariasis vectors is promising and needs a semifield and small scale filed trials.

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Section: Introductionmentioning

confidence: 99%

Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Baskar

Ragavendran

Pandy

et al. 2020

Heliyon

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“…However, more studies show that a combination of entomopathogenic fungi with other compounds increases virulence toward insect in which B. bassiana and acaricidal showed enhanced acaricidal activity [53]. In current study, the oviposition rate was very low in ticks treated with A. oryzae compared to control in which high number of eggs were laid this could be due to infertility effect caused by entomopathogenic fungi [51]. Despite of A. gemma having the highest weight than R. appendiculatus and H. anatolicum, high number of eggs were laid by Hyalomma species due to high fecundity rate at warm laboratory condition in which other studies revealed similar situation.…”

Section: Sci Forschenmentioning

confidence: 55%

“…This study revealed that A. oryzae was more virulent compared to control on ticks although the infectivity increased with rise in concentration in all species of ticks. The mode of action was through cuticle fungal penetration and infection as Aspegillus species like other fungal spp developed a symbiotic relationship with host ticks and caused pathogenic effect [51].…”

Section: Sci Forschenmentioning

confidence: 99%

Susceptibility of Different Species of Ticks (Acari: Ixodidae) to an Entomopathogenic Fungus in Tanzania

Zekeya¹,

Er²,

Ndossi³

2020

J Anim Sci Res

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Volume 4 -Issue 2 of leather products affecting economy of the country [15][16][17]. Impacts of ticks do not end on large mammals but also small ruminant and birds including ostrich leading to severe skin damage and loss [13,[18][19][20][21]. However, poor animal keeping and grazing method such as free range landraces especially in pastoralist communities have been reported to amplify the problem in Africa including Tanzania [22][23][24]. The hard ticks especially the A. gemma and R. appendiculatus are the main cause of skins and hides damage in Africa [25,26] whereas H. marginatum infest bird skin as well. The overall effect of tick on animals and birds lead to downgrading of hides and skins and finally rejection by processing industries [16,15]. In Somalia and Ethiopia for instance, Ticks and Tick-Borne Diseases (TTBDs) have been reported to have high impact on tannery industry leading to sluggish in leather industry [27][28][29].Management of ticks has been solely based on chemicals through spray and dipping of animals in chemical such as organophosphate

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“…Furthermore, the hatching percentage was found to relate inversely with product concentration. This may be attributed to infertility effects caused by entomopathogenic fungi (Ghany, 2015).Further it has been reported that the fungus impairs ovary development, thus, egg production rates (Ghany, 2015;Wasinpiyamongkol and Kanchanaphum, 2019). These findings suggest that the use of A. oryzae may reduce the tick population with subsequent reduction of the prevalence and burden of tick-borne diseases.…”

Section: Discussionmentioning

confidence: 98%

Entomopathogenic fungi (Aspergillus oryzae) as biological control agent of cattle ticks in Tanzania

Sylvia¹,

Zekeya²,

Esther³

et al. 2022

J. Vet. Med. Anim. Health

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Ticks are the most important ectoparasites that are responsible for severe economic losses in livestock industry. The use of chemical acaricides is the most common method used to control ticks in livestock. This study was conducted to determine the efficacy of Aspergillus oryzae as an alternative biological agent in controlling ticks to enhance livestock productivity. The efficacy of A. oryzae at different concentrations was evaluated against larvae and adults of the hard tick genera Rhipicephalus, Boophilus, and Amblyomma using an immersion test under laboratory conditions. Field trials were conducted in two purposively selected cattle herds in Monduli district, northern Tanzania. A. oryzae at a concentration of 1 × 10 6 conidial/ml was sprayed on all cattle tick-infested areas. The results demonstrated a concentration-related increase in mortality for both larvae and adult female engorged ticks. The mean mortality of larvae and female engorged ticks was statistically significant at p ˂ 0.05 and p ˂ 0.001, respectively. Egg production was found to decrease with increased A. oryzae concentration. Additionally, there was a statistically significant difference in egg production index and oviposition reduction (p = 0.009) while there was no significant difference in egg hatching and product effectiveness at p = 0.089 and p = 0.004, respectively between the tested ticks' genera. Under field conditions, the bio-acaricide demonstrated a statistically significant tick reduction in all the treated cattle. This study concludes that A. oryzae has good acaricidal activity against ticks and hence, is one of the potential tick control methods for sustainable tick control schemes.

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Isolating and identifying fungi to determine whether their biological properties have the potential to control the population density of mosquitoes

Cited by 14 publications

References 24 publications

Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Larvicidal and histopathology effect of endophytic fungal extracts of Aspergillus tamarii against Aedes aegypti and Culex quinquefasciatus

Susceptibility of Different Species of Ticks (Acari: Ixodidae) to an Entomopathogenic Fungus in Tanzania

Entomopathogenic fungi (Aspergillus oryzae) as biological control agent of cattle ticks in Tanzania

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