2001
DOI: 10.1002/1439-7633(20010105)2:1<45::aid-cbic45>3.0.co;2-#
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Isolation and Amino Acid Sequence of a Serine Proteinase Inhibitor from Common Flax ( Linum usitatissimum ) Seeds

Abstract: LUTI (Linum usitatissimum trypsin inhibitor), a member of the potato inhibitor I family, has been isolated from seeds of flax by ethanol fractionation, ion exchange chromatography on CM-Sephadex C-25, affinity purification on immobilized methylchymotrypsin (alpha-chymotrypsin in which His57 has been converted to 3-methylhistidine) in the presence of 5M NaCl, and finally by reversed-phase HPLC. The 7655 Da inhibitor consists of a single polypeptide chain of 69 residues with one disulfide bridge. The molecule is… Show more

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Cited by 12 publications
(4 citation statements)
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“…Proteins were precipitated with 80% ethanol and collected by centrifugation, solubilized in distilled water, dialysed against 0.1 M acetate buffer, pH 5.5, and applied to a CM-Sephadex C-25 column. The inhibitor from seedlings, contrary to the preparation obtained from resting seeds (Lorenc-Kubis et al 2001), was not very well separated from inactive proteins (Fig. 2).…”
Section: Isolation Of the Inhibitor From The Seedlingsmentioning
confidence: 84%
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“…Proteins were precipitated with 80% ethanol and collected by centrifugation, solubilized in distilled water, dialysed against 0.1 M acetate buffer, pH 5.5, and applied to a CM-Sephadex C-25 column. The inhibitor from seedlings, contrary to the preparation obtained from resting seeds (Lorenc-Kubis et al 2001), was not very well separated from inactive proteins (Fig. 2).…”
Section: Isolation Of the Inhibitor From The Seedlingsmentioning
confidence: 84%
“…From 320 g of 6-day-old seedlings we isolated the trypsin inhibitor following the previously published procedure (Lorenc-Kubis et al 2001). The seedlings were homogenised with 5 volumes (w/v) of 0.1 M sodium acetate buffer, pH 5.1, stirred for 1 hour at 4°C, then clarified by centrifugation.…”
Section: Isolation Of the Inhibitor From The Seedlingsmentioning
confidence: 99%
See 2 more Smart Citations