Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane

Shimazaki, Youji; Hirose, Yu

doi:10.1016/j.jchromb.2014.09.021

Journal of Chromatography B

2014

DOI: 10.1016/j.jchromb.2014.09.021

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Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane

Youji Shimazaki

Yu Hirose

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Cited by 5 publications

References 14 publications

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Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta

Shimazaki

Takatsu

2015

Appl Biochem Biotechnol

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Amyloid beta 1-40 peptide was specifically isolated and analyzed from human plasma spiked with amyloid beta using a combined method of biotinylated anti-amyloid beta antibody binding to membrane-immobilized avidin (immunoaffinity membrane) and matrix-assisted laser desorption /ionization time-of-flight mass spectrometry (MALDI-TOF MS). A solution of 10 μL containing 13.6 ng to 2.9 μg of amyloid beta peptide was examined in this method. After the isolated amyloid beta peptide from the spiked human plasma containing 2.9 μg of amyloid beta peptide was incubated in the presence of trifluoroacetic acid, fibrillization of the peptides was observed using a thioflavin T assay. Furthermore, an immunoaffinity membrane present on the inner wall of a tube (diameter 2 mm) captured the amyloid beta peptide from the spiked human plasma. Our results indicate that the combination of the immunoaffinity membrane procedure and MALDI-TOF MS can be used to capture and analyze the target antigens such as amyloid beta in micro-spaces.

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Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta

Shimazaki

Takatsu

2015

Appl Biochem Biotechnol

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Combining iontronic, chromatography and nanopipette for Aβ42 aggregates detection and separation

Moderne,

Abrao-Nemeir,

Meyer

et al. 2023

Analytica Chimica Acta

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Inhibition of Amyloid β Protein Fibrillation via Carboxypeptidase Y after Protein Trapping Using Immunoaffinity Membranes

Saito¹,

Zako²,

Takahashi³

et al. 2016

Chem. Lett.

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Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane

Cited by 5 publications

References 14 publications

Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta

Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta

Combining iontronic, chromatography and nanopipette for Aβ42 aggregates detection and separation

Inhibition of Amyloid β Protein Fibrillation via Carboxypeptidase Y after Protein Trapping Using Immunoaffinity Membranes

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