Isolation and Analysis of Cell Wall Polymers from Olive Pulp

Coimbra, Manuel A.; Delgadillo, Ivonne; Waldron, Keith W.; Selvendran, Robert R.

doi:10.1007/978-3-642-60989-3_2

Cited by 117 publications

(134 citation statements)

References 53 publications

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“…The Et50 and Et75 samples were dissolved in 50 mM potassium phosphate buffer pH 6.5 (1.0 mg/mL) and sequentially eluted in the same phosphate buffer, and buffer with 0.125, 0.250, 0.500, and 1.00 M NaCl. Fractions (3.0 mL) were collected and assayed for sugars according to a modification of phenol-concentrated sulfuric acid method (Coimbra, Delgadillo, Waldron, & Selvendran, 1996). The fractions of interest were pooled, dialysed and freeze-dried.…”

Section: Deae-sepharose Ff Anion Exchange Chromatographymentioning

confidence: 99%

“…Glycosidic linkage analysis was performed by methylation of the polysaccharides in order to obtain partially methylated alditol acetates that were subsequently analysed by gas chromatography-mass spectrometry (GC-MS), as described by Nunes and Coimbra (2001). Methylated polysaccharides were also carboxyl reduced with LiAlD 4 , as described by Coimbra et al (1996), for identification of the uronic acids and determination of their linkages.…”

Section: Sugar and Linkage Analysismentioning

confidence: 99%

“…The samples were eluted with 50 mM acetate buffer, pH 5.0. Fractions (3.0 mL) were collected and assayed for sugars by the phenol-sulphuric acid method (Coimbra et al, 1996). The fractions of interest were pooled, dialysed and freeze-dried.…”

Section: Determination Of the Methyl Esterification Degreementioning

confidence: 99%

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In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

Martins

Simões

Ferreira

et al. 2017

Carbohydrate Polymers

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a b s t r a c tPterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60-75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endopolygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.

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Section: Deae-sepharose Ff Anion Exchange Chromatographymentioning

confidence: 99%

Section: Sugar and Linkage Analysismentioning

confidence: 99%

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In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

Martins

Simões

Ferreira

et al. 2017

Carbohydrate Polymers

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“…Neutral sugars were released by Saeman hydrolysis and analysed as their alditol acetates by gas chromatography [23,24] using a FISONS 8340 chromatograph with a split injector (split ratio 1:60) and a FID detector. A DB-225 column (Agilent J&W, USA; 30 m Â 0.25 mm Â 0.15 lm) was used.…”

Section: Sugar Analysismentioning

confidence: 99%

“…The flow rate of the carrier gas (H 2 ) was set at 1 mL/min at 200°C. Uronic acids (UA) were determined colorimetrically according to Coimbra et al [23]. The hydrolysis of all samples was done in duplicate and each sample was injected twice.…”

Section: Sugar Analysismentioning

confidence: 99%

Improved efficiency of brewer’s spent grain arabinoxylans by ultrasound-assisted extraction

Reis¹,

Coelho

Coimbra

et al. 2015

Ultrasonics Sonochemistry

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Characterisation of Rosa Mosqueta seeds: cell wall polysaccharide composition and light microscopy observations

Dourado

Vasco

Gama

et al. 2000

J. Sci. Food Agric.

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The utilisation of enzymes for the extraction of vegetable oils from seeds has been a topic of growing interest in recent years. Knowledge of the cell wall polysaccharide composition is important to select the enzyme(s) necessary for the most effective degradation of the cell walls. The purpose of the present work is to characterise the seeds of Rosa Mosqueta (Rosa aff rubiginosa) by light microscopy (where several differential staining methods were applied to analyse the seed structure) and by the isolation of cell wall polysaccharide extracts. The mature seed of Rosa Mosqueta has a very thick and structurally complex seed coat comprising heavily ligni®ed tissue. The embryo has two cell layers of remaining endosperm tissue (indicating that this is an exalbuminous seed), two voluminous cotyledons that contain the oil, and bundles of provascular tissues distributed perpendicularly to the transverse axis of the embryo. The major non-cellulosic polysaccharides from the non-ligni®ed tissues are glucuronoxylans and pectic polysaccharides; glucans are also present in small amounts. The major non-cellulosic polysaccharides from the ligni®ed tissues are glucuronoxylans. Concerning the use of enzymes for oil extraction, microscopy and cell wall polysaccharide analysis showed that the use of pectic enzymes followed by a xylanase or a cellulase should be explored.

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Isolation and Analysis of Cell Wall Polymers from Olive Pulp

Cited by 117 publications

References 53 publications

In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

Improved efficiency of brewer’s spent grain arabinoxylans by ultrasound-assisted extraction

Characterisation of Rosa Mosqueta seeds: cell wall polysaccharide composition and light microscopy observations

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