Isolation and Anti-Inflammatory Activity of Bakuchiol from<i>Ulmus davidiana</i>var.<i>japonica</i>

Choi, Sang Yoon; Lee, Sang‐Hyun; Choi, Won-Hee; Lee, Yeonmi; Jo, Youn Ock; Ha, Tae‐Youl

doi:10.1089/jmf.2009.1207

Cited by 63 publications

(38 citation statements)

References 23 publications

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“…Regarding the active compounds included in BEE, several candidate compounds such as bakuchiol, mansonone E and F, glycoproteins, and catechins were documented [2,20,27,30]. Those compounds are known to have diverse biological effects including anti-inflammatory, proapoptotic, and anti-oxidative properties according to the isolation method or type of cell line [20,30].…”

Section: Discussionmentioning

confidence: 99%

“…Those compounds are known to have diverse biological effects including anti-inflammatory, proapoptotic, and anti-oxidative properties according to the isolation method or type of cell line [20,30]. In particular, bakuchiol and masonone E and F are known to be major components in ethanolic BEE that exert anti-inflammatory or pro-apoptotic effects [2]. Therefore, we assume that these compounds might have been included in the BEE and influenced our results.…”

Section: Discussionmentioning

confidence: 99%

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Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity

et al. 2012

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Control of inflammation is widely accepted as an important strategy for cancer chemoprevention. Anti-inflammatory effects of bark extracts of elm tree (BEE) have been amply reported. Therefore, BEE may be a good candidate cancer chemopreventive agent. Considering the high incidence of hepatic cancer and limited therapeutic approaches for treating this disease, it is important to develop liver cancer-specific chemopreventive agents. To evaluate the chemopreventive potential of BEE, we investigated the growth inhibition effect of BEE on the HepG2 human hepatocellular carcinoma cell line. We performed a cell counting kit-8 assay to determine cell viability, and 4,6-diamino-2-phenylindole staining and flow cytometry to measure apoptotic cell death. Finally, the expression levels of pro- and anti-apoptotic proteins were measured. BEE inhibited the growth of HepG2 cells and induced apoptosis in a dose-dependent manner. Pro-apoptotic activity was promoted via the mitochondrial pathway of apoptosis, as demonstrated by the activation of pro-apoptotic proteins Bax, caspase-9, caspase-3, and poly (ADP-ribose) polymerase as well as the down-regulation of the anti-apoptotic protein Bcl-2. These results suggest that BEE may have potential use in hepatic cancer chemoprevention by suppressing cancer cell growth via pro-apoptotic activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity

et al. 2012

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“…(1-(4-hydroxyphenyl)-3,7-dimethyl-3-vinyl-1,6-octadiene, Figure 1), a monoterpene phenol present in seeds and leaves of Psoralea corylifolia (Leguminosae), shows many pharmacological properties such as anti-pyretic (Backhouse et al, 2001), anti-inflammatory (Choi et al, 2010), anti-aging (Chaudhuri & Bojanowski, 2014) and antimicrobial activities (Katsura et al, 2001). More recently, it has been demonstrated that bakuchiol shows considerable anti-tumor effects against human lung adenocarcinoma A549 cells (Chen et al, 2010), SK-MEL-2 human melanoma cells (Madrid et al, 2015) and skin cancer cells (Kim et al, 2016).…”

Section: Bakuchiolmentioning

confidence: 99%

Identification of UDP-glucuronosyltransferases 1A1, 1A3 and 2B15 as the main contributors to glucuronidation of bakuchiol, a natural biologically active compound

Wang

et al. 2016

Xenobiotica

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1. Bakuchiol, one of the main active compounds of Psoralea corylifolia, possesses a variety of pharmacological activities such as anti-tumor and anti-aging effects. Here, we aimed to characterize the glucuronidation of bakuchiol using human liver microsomes (HLM) and expressed UDP-glucuronosyltransferase (UGT) enzymes. 2. The glucuronide of bakuchiol was confirmed by liquid chromatography-mass spectrometry (LC-MS) and β-glucuronidase hydrolysis assay. Glucuronidation rates and kinetic parameters were derived by enzymatic incubation and model fitting. Activity correlation analyses were performed to identify the main UGT isoforms contributing to hepatic metabolism of bakuchiol. 3. Among the three UGT enzymes (i.e., UGT1A1, UGT1A3 and UGT2B15) capable of catalyzing bakuchiol glucuronidation, UGT2B15 showed the highest activity with a CL value of 100 μl/min/nmol. Bakuchiol glucuronidation was strongly correlated with glucuronidation of 5-hydroxyrofecoxib (r = 0.933; p < 0.001), 3-O-glucuronidation of β-estradiol (r = 0.719; p < 0.01) and significantly correlated with 24-O-glucuronidation of CDCA (r = 0.594; p < 0.05). In addition, a marked species difference existed in hepatic glucuronidation of bakuchiol. 4. In conclusion, UGT1A1, UGT1A3 and UGT2B15 were identified as the main contributors to glucuronidation of bakuchiol.

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“…Ulmus davidiana var. japonica (UJ) has been planted widely in northern Japan and is used as a traditional medicine for its anti-inflammatory, anti-glycation, and anti-angiogenic activities; further, it exerts protective effects against glutamate-induced neurotoxicity and sepsis (Lee and Kim 2001; Lee et al 2005; Choi et al 2010; Jung et al 2007; Zheng et al 2011). Recently, a new technique has been developed for the production of ultrafine (smaller than 0.1 μm) particles of medicinal herbs.…”

Section: Introductionmentioning

confidence: 99%

Ultrafine particles of Ulmus davidiana var. japonica induce apoptosis of gastric cancer cells via activation of caspase and endoplasmic reticulum stress

2014

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Small-sized particles are more suitable for targeted delivery and are therapeutically more effective than large-sized particles. In this study, we investigated the anticancer effects of ultrafine particles of Ulmus davidiana var. japonica (ufUJ) on human gastric cancer cell lines SNU-1, SNU-216, and SNU-484. ufUJ induced apoptosis by the proteolytic activation of caspase-9, caspase-6, and caspase-3 and cleavage of poly (ADP-ribose) polymerase. The expression levels of the endoplasmic reticulum stress-related protein BiP markedly increased after ufUJ treatment. BiP knockdown decreased ufUJ-induced cell death. ufUJ-induced apoptosis was inhibited by the caspase-3 inhibitor z-DEVD-fmk, caspase-6 inhibitor z-VEID-fmk, and caspase-9 inhibitor z-LEHD-fmk, and by siRNAs against caspases 3, 6, and 9. Gastric cancer cells did not show anchorage-independent growth in the presence of ufUJ. However, cells treated with caspase inhibitors showed an enhanced colony-forming ability. These findings may be helpful in the prevention of gastric cancer and in the development of functional foods.

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Isolation and Anti-Inflammatory Activity of Bakuchiol fromUlmus davidianavar.japonica

Cited by 63 publications

References 23 publications

Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity

Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity

Identification of UDP-glucuronosyltransferases 1A1, 1A3 and 2B15 as the main contributors to glucuronidation of bakuchiol, a natural biologically active compound

Ultrafine particles of Ulmus davidiana var. japonica induce apoptosis of gastric cancer cells via activation of caspase and endoplasmic reticulum stress

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