Isolation and antimicrobial resistance of vancomycin resistant Enterococcus spp. (VRE) and methicillin-resistant S. aureus (MRSA) on beef and chicken meat, and workers hands from slaughterhouses and retail shops in Turkey

Telli, Nihat; TELLI, AE; Biçer, Yusuf; Turkal, Gamze; Uçar, Gürkan

doi:10.12681/jhvms.29373

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…In this study, E. hirae was the predominantly isolated species, which accounted for 37.7% of total Enterococcus isolates, and the remaining 33.3%, 15.5%, 12.2%, and 1.1% of the isolates were identified as E. casseliflavus, E. faecium, E. faecalis, and E. gallinarum, respectively (Table 1). Wide variation (0-90.6%) in the prevalence of Enterococci in food-producing animals has been reported in different countries [1,4,5,12,16,[31][32][33]. In the present study, the speciation of the isolates confirmed that E. hirae was the most prevalent species identified from sheep and goat carcass samples.…”

Section: Discussionsupporting

confidence: 71%

Enterococci<em> </em>from Food-producing Animals at a Slaughterhouse: Antibiotic Resistance and Genetic Diversity in Turkey

Cebeci

2024

Preprint

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Enterococci are widely acknowledged as prominent pathogens in hospitals, with the potential to transfer resistance genes, virulence factors, or other characteristics that increase their ability to survive in humans. Healthy cattle, sheep, and goats can be reservoirs for gastrointestinal pathogenic fecal enterococci, some of which could be multidrug-resistant to antimicrobials. The objective of this study was to determine the prevalence and diversity of Enterococcus species in healthy sheep, goat, and cattle carcasses, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2019–2020, carcass surface samples of 150 ruminants were collected in a slaughterhouse. A total of 90 enterococci, comprising 5 species, were obtained. The overall prevalence of enterococci was found to be 60%, out of which 37.7% were identified as E. hirae, 33.3% as E. casseliflavus, 15.5% as E. faecium, 12.2% as E. faecalis, and 1.1% as E. gallinarum. Virulence-associated genes of efaA (12.2%) were commonly observed in the Enterococcus isolates, followed by gelE (3.3%), asaI (3.3%), and ace (2.2%). Characteristic resistance genes were identified by PCR with an incidence of 6.6%, 2.2%, 1.1%, 1.1%, 1.1%, and 1.1% for the tetM, ermB, ermA, aac(6’)Ie-aph(2")-la VanC1, and VanC2 genes, respectively. The high resistance to quinupristin-dalfopristin (28.8%), tetracycline (21.1%), ampisilin (20%), and rifampin (15.5%) was distributed among two, four, four, and five of the five species, respectively. The resistance of Enterococcus isolates to 11 antibiotic groups was determined and multidrug-resistant (MDR) strains occupied 18.8% of the Enterococcus isolates. Efflux pump genes causing multidrug resistance were detected in Enterococcus isolates (34.4%). The study's results showed that there were enterococci in the slaughterhouse that were multidrug resistant (MDR) and had a number of genes linked to virulence that could be harmful to human health.

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Section: Discussionsupporting

confidence: 71%

Enterococci<em> </em>from Food-producing Animals at a Slaughterhouse: Antibiotic Resistance and Genetic Diversity in Turkey

Cebeci

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In this study, E. hirae was the predominantly isolated species, which accounted for 37.7% of total Enterococcus isolates, and the remaining 33.3%, 15.5%, 12.2%, and 1.1% of the isolates were identified as E. casseliflavus, E. faecium, E. faecalis, and E. gallinarum, respectively (Table 3). Wide variation (0-90.6%) in the prevalence of Enterococci in food-producing animals has been reported in different countries [1,4,5,12,16,[31][32][33]. In the present study, the speciation of the isolates confirmed that E. hirae was the most prevalent species identified from sheep and goat carcass samples.…”

Section: Discussionsupporting

confidence: 71%

Enterococci in Ruminant Carcasses at a Slaughterhouse: Prevalence, Virulence Factors and Antibiotic Resistance in Turkey

Cebeci

2024

Preprint

View full text Add to dashboard Cite

Enterococci are widely acknowledged as prominent pathogens in hospitals, with the potential to transfer resistance genes, virulence factors, or other characteristics that increase their ability to survive in humans. The objective of this study was to determine the prevalence and diversity of Enterococcus species in healthy sheep, goat, and cattle carcasses, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2019–2020, carcass surface samples of 150 ruminants were collected in a slaughterhouse. A total of 90 enterococci, comprising 5 species, were obtained. The overall prevalence of enterococci was found to be 60%, out of which 37.7% were identified as E. hirae, 33.3% as E. casseliflavus, 15.5% as E. faecium, 12.2% as E. faecalis, and 1.1% as E. gallinarum. Virulence-associated genes of efaA (12.2%) were commonly observed in the Enterococcus isolates, followed by gelE (3.3%), asaI (3.3%), and ace (2.2%). Characteristic resistance genes were identified by PCR with an incidence of 6.6%, 2.2%, 1.1%, 1.1%, 1.1%, and 1.1% for the tetM, ermB, ermA, aac(6’)Ie-aph(2")-la VanC1, and VanC2 genes, respectively. The high resistance to quinupristin-dalfopristin (28.8%), tetracycline (21.1%), ampisilin (20%), and rifampin (15.5%) was distributed among two, four, four, and five of the five species, respectively. The resistance of Enterococcus isolates to 11 antibiotic groups was determined and multidrug-resistant (MDR) strains occupied 18.8% of the Enterococcus isolates. The study's results showed that there were enterococci in the slaughterhouse that were multidrug resistant (MDR) and had a number of genes linked to virulence that could be harmful to human health.

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“…This research is particularly relevant because of the high consumption of meat by a significant portion of the local population. Wide variation (0–90.6%) in the prevalence of enterococci in food-producing animals has been reported in this study and different countries 1 , 4 , 5 , 12 , 16 , 35 – 37 . In the present study, the speciation of the isolates confirmed that E. hirae was the most prevalent species identified from sheep and goat carcass samples.…”

Section: Discussionsupporting

confidence: 54%

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

Cebeci

2024

Sci Rep

View full text Add to dashboard Cite

Healthy cattle, sheep, and goats can be reservoirs for gastrointestinal pathogenic fecal enterococci, some of which could be multidrug-resistant to antimicrobials. The objective of this study was to determine the prevalence and diversity of Enterococcus species in healthy sheep, goat, and cattle carcasses, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2019–2020, carcass surface samples were collected from 150 ruminants in a slaughterhouse. A total of 90 enterococci, comprising five species, were obtained. The overall prevalence of enterococci was found to be 60%, out of which 37.7% were identified as Enterococcus (E.) hirae, 33.3% as E. casseliflavus, 15.5% as E. faecium, 12.2% as E. faecalis, and 1.1% as E. gallinarum. Virulence-associated genes of efaA (12.2%) were commonly observed in the Enterococcus isolates, followed by gelE (3.3%), asaI (3.3%), and ace (2.2%). High resistance to quinupristin-dalfopristin (28.8%), tetracycline (21.1%), ampicillin (20%), and rifampin (15.5%) was found in two, four, four, and five of the Enterococcus species group, respectively. The resistance of Enterococcus isolates to 11 antibiotic groups was determined and multidrug resistant (MDR) strains were found in 18.8% of Enterococcus isolates. Characteristic resistance genes were identified by PCR with an incidence of 6.6%, 2.2%, 1.1%, 1.1%, 1.1%, and 1.1% for the tetM, ermB, ermA, aac(6ʹ)Ie-aph(2")-la, VanC1, and VanC2 genes in Enterococcus isolates, respectively. Efflux pump genes causing multidrug resistance were detected in Enterococcus isolates (34.4%). The results showed that there were enterococci in the slaughterhouse with a number of genes linked to virulence that could be harmful to human health.

show abstract

Isolation and antimicrobial resistance of vancomycin resistant Enterococcus spp. (VRE) and methicillin-resistant S. aureus (MRSA) on beef and chicken meat, and workers hands from slaughterhouses and retail shops in Turkey

Cited by 5 publications

References 37 publications

Enterococci<em> </em>from Food-producing Animals at a Slaughterhouse: Antibiotic Resistance and Genetic Diversity in Turkey

Enterococci<em> </em>from Food-producing Animals at a Slaughterhouse: Antibiotic Resistance and Genetic Diversity in Turkey

Enterococci in Ruminant Carcasses at a Slaughterhouse: Prevalence, Virulence Factors and Antibiotic Resistance in Turkey

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

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