Isolation and Antioxidant Activity of Methyl Aconitates from Arctic Red Alga Polysiphonia stricta

Abstract

: In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.

Antioxidant Capacity of Crude Extract and Its Solvent Fractions of Arctic Terrestrial Plant Ranunculus heperporeus

“…과도한 산화적 스트레스는 인체 내의 superoxide anion (O 2 − •), hydroxyl(HO•), alkoxyl(RO•), hydroperoxyl radical (HOO•) 등과 같은 활성 산소종(reactive oxygen species, ROS)이나 nitric oxide(NO•)와 peroxynitrite(ONOO − ) 같 은 활성질소종(reactive nitrogen species, RNS)의 생성을 야기한다 (Fang et al 2002) (Aruoma 1998;Diplock et al 1998;Halliwell 1996;Ichiniose 2003 (Azuma et al 1999;Frankel et al 1993;Giese 1996;Rein et al 2000;Rice-Evans et al 1996 (Ha et al 2006;Krapp et al 2009;Lee et al 2009Lee et al , 2014Regoli et al 2000;Seo et al 2007) (Halliwell et al 1995;Kim et al 2012;Rice-Evans et al 1997 (Li et al 2009;Negro et al 2003;Ramandeep and Geoffrey 2005…”