Isolation and biochemical characterization of a thaumatin-like kiwi allergen

Gavrović-Jankulović, Marija; Ćirković, Tanja; Vučković, Olga; Atanasković-Marković, Marina; Petersen, Arnd; Gojgić, Gordana; Burazer, Lidija; Jankov, Ratko M.

doi:10.1067/mai.2002.128947

Cited by 109 publications

(117 citation statements)

References 38 publications

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“…We have reported previously that malting barley TLPs isoforms are glycosylated with different sugar content, TLP-S being two fold higher and with the ability to bind concanavalin A 8 . It is worthy to note that the number of newly recognized glycoproteins among the TLPs family is growing 13,16,18,19,23,26,37 . We have demonstrated TLP-S has higher antifungal and antibacterial activity than TLP-R, regarding the IC 50 value.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Gorjanović

Beljanski

Gavrović-Jankulović

et al. 2007

Journal of the Institute of Brewing

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Two basic proteins were isolated to homogeneity from malting barley (Hordeum vulgare L.) grain. Proteins were identified as members of a Thaumatin-Like Protein (TLP) family, by Western blot. Isoforms, assigned as TLP-S and TLP-R, have slightly different mobility at about 22 and 27 kDa in nonreducing and reducing conditions, and pI values of 9.5 and 9.4, respectively. The antifungal potency of malting barley grain TLPs isoforms was examined on Micrococcus lysodeikticus, Saccharomyces cerevisiae, Candida albicans and plant pathogen Fusarium sporotrichioides growth in vitro. It was found that that IC 50 value for TLP-S was two fold higher. Antibacterial and antifungal activities of both isoforms were completely abolished by divalent (Ca 2+ , Mn 2+ , Mg 2+ ) and monovalent (K + ) cations, at concentrations approximating physiological ionic strength and higher. Glucanase activity was not observed; neither TLP-S nor TLP-R digested glucan. On the basis of these results, the importance of TLP for barley grain protection against fungal diseases has been discussed together with the mechanism of antimicrobial action.

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Section: Discussionmentioning

confidence: 99%

“…Immunodetection was essentially performed according to the method of Harlow and Lane 14 . In brief, a monoclonal antibody BG148 13 was used as the primary antibody, and alkaline phosphatase anti-mouse IgG (Sigma Chemical Co, St. Louis, USA) was used as the secondary antibody.…”

Section: Immunodetection By Western Blotmentioning

confidence: 99%

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Gorjanović

Beljanski

Gavrović-Jankulović

et al. 2007

Journal of the Institute of Brewing

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“…Mal d 2 from apple (Malus domestica) was the first allergen described as a TLP (28). Following the identification of Mal d 2, several fruit allergens, including Pru av 2 from cherry (Prunus avium), Act c 2 and Act d 2 from kiwi (Actinidia chinensis and A. deliciosa, respectively), Cap a 1 from bell pepper (Capsicum annuum) and thaumatin from grape (Vitis vinifera), were reported as allergens belonging to the PR5 family (19,(29)(30)(31)(32). Recently, PR-5 allergens from pollen have been identified as Jun a 3 from mountain cedar (J. ashei), Jun v 3 from eastern red cedar (J. virginiana) and Cup a 3 from C. arizonica (20,22,33).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen

Fujimura

Futamura

Midoro-Horiuti

et al. 2007

Allergy

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Background-Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 was cloned as a homologue of Jun a 3, which is a major allergen from mountain cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and there are no reports on its allergenic activity. The aims of this study were to isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in patients with Japanese cedar pollinosis.

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“…They have been recently identified as allergens in apple [93], cherry [94, 95], bell pepper [96], kiwi [97] and grapes [87]. Their real clinical significance remains ill-defined due to the extreme rarity of monosensitized patients (in the recent EU-funded study SAFE, only one of 400 apple-allergic patients from four European countries was monosensitized to TLP).…”

Section: Allergy To Thaumatin-like Proteinmentioning

confidence: 99%

Plant Food Allergies: A Suggested Approach to Allergen-Resolved Diagnosis in the Clinical Practice by Identifying Easily Available Sensitization Markers

Asero¹

2005

Int Arch Allergy Immunol

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Background: Molecular biology techniques have led to the identification of a number of allergens in vegetable foods, but due to the lack of purified food proteins for routine diagnostic use, the detection of sensitizing allergens remains a nearly impossible task in most clinical settings. The allergen-resolved diagnosis of food allergy is essential because each plant-derived food may contain a number of different allergens showing different physical/chemical characteristics that strongly influence the clinical expression of allergy; moreover, many allergens may cross-react with homologue proteins present in botanically unrelated vegetable foods. Objective: Through a review of the available literature, this study aimed to detect ‘markers’ of sensitization to specific plant food allergens that are easily accessible in the clinical practice. Results: There are several ‘markers’ of sensitization to different allergenic proteins in vegetable foods that can be helpful in the clinical practice. Specific algorithms for patients allergic to Rosaceae and to tree nuts were built. Conclusion: Clinical allergologists lacking the assistance of an advanced molecular biology lab may take advantage of some specific clinical data as well as of some ‘markers’ in the difficult task of correctly diagnosing patients with plant food allergy and to provide them the best preventive advice.

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Isolation and biochemical characterization of a thaumatin-like kiwi allergen

Cited by 109 publications

References 38 publications

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Antimicrobial Activity of Malting Barley Grain Thaumatin-Like Protein Isoforms, S and R

Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen

Plant Food Allergies: A Suggested Approach to Allergen-Resolved Diagnosis in the Clinical Practice by Identifying Easily Available Sensitization Markers

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