Isolation and biological characterization of mesenchymal stem cells from goose dermis

Abstract

The skin is a natural target of stem cell research because of its large size and easy accessibility. Cutaneous mesenchymal stem cells have shown to be a promising source of various adult stem cell or progenitor cell populations, which provide an important source of stem cell-based investigation. Nowadays, much work has been done on dermal-derived mesenchymal stem cells (DMSCs) from humans, mice, sheep, and other mammals, but the literature on avian species has been rarely reported. As an animal model, the goose is an endemic species abounding in dermal tissues which is important in the global economy. In this study, we isolated and established the mesenchymal stem cell line from dermis tissue of goose, which were subcultured to passage 21 in vitro without loss of their functional integrity in terms of morphology, renewal capacity, and presence of mesenchymal stem cell markers. Cryopreservation and resuscitation were also observed in different passages. To investigate the biological characteristics of goose DMSCs, immunofluorescence, reverse transcription-polymerase chain reaction, and flow cytometry were used to detect the characteristic surface markers. Growth curves and the capacity of colony forming were performed to test the self-renew and proliferative ability. Furthermore, the DMSCs are induced to osteoblasts, adipocytes, and chondrocytes in vitro. Our results suggest that DMSCs isolated from goose embryos possess similar biological characteristics to those from other species. The methods in establishment and cultivation of goose DMSCs line demonstrated a good self-renew and expansion potential in vitro, which provided a technological platform for preserving the valuable genetic resources of poultry and a great inspiration for in vitro investigation of avian MSCs.

Tendon-Derived Mesenchymal Stem Cells (TDSCs) as an In Vitro Model for Virological Studies in Wild Birds

“…In this work, we differentiated TDSCs from blackbirds in vitro under the action of specific induction factors into osteogenic, adipogenic, and chondrogenic lineages. The differentiation was assessed using specific staining procedures, i.e., Alizarin Red S staining, Oil Red O staining, and Alcian Blue staining, respectively, as validated in other MSCs models of avian origin [ 12 , 28 , 29 , 44 ].…”

Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells

“…In the era of stem cell research, mesenchymal stem cells have been one of the key sources of cell-based therapies [51]. Different research groups identified, isolated, and compared the cellular morphology, surface markers, and differentiation abilities of MSCs derived from different sources [2][3][4][5][6][7][8][9][10][11][12][13][14][15]. A lot of work has been performed to date to investigate the possible therapeutic effects of MSCs in different acute kidney injury models [16][17][18][19][20][21][22][23][24].…”

“…MSCs are undifferentiated, self-renewable, non-hematopoietic adult stem cells of mesodermal origin [1]. These multipotent stem cells are broadly distributed in the body and can be relatively easily isolated from various tissues, such as bone marrow, umbilical cord blood, fat, muscle, periosteum, lung, testis, and dermis [2][3][4][5][6][7][8][9][10][11][12][13][14]. MSCs can differentiate into both mesenchymal and non-mesenchymal lineages, including differentiation toward adipocytes, chondrocytes, osteocytes, skeletal muscle cells, renal cells, and smooth muscle cells [9][10][11]15].…”

PRR34‐AS1 overexpression promotes protection of propofol pretreatment against ischemia/reperfusion injury in a mouse model after total knee arthroplasty via blockade of the JAK1‐dependent JAK‐STAT signaling pathway

“…The cartilage tissue at the knee joints of the mice was isolated, cultured, and identified (Chan et al, 2018;Wang et al, 2018).…”